Analyze Diet

Topic:Statistical Analysis

Statistical analysis in equine research involves the application of mathematical techniques to collect, review, and interpret data related to horse health, behavior, and performance. This field encompasses a variety of methods, including descriptive statistics, inferential statistics, and multivariate analysis, to identify patterns, test hypotheses, and draw conclusions from equine data. Statistical analysis aids in understanding various aspects of equine science, such as disease prevalence, treatment efficacy, genetic traits, and performance metrics. This page aggregates peer-reviewed research studies and scholarly articles that explore the methodologies, applications, and outcomes of statistical analysis in the context of equine studies.
Equine lameness detection and monitoring during diagnostic anaesthesia with an instrumented hoof boot.
Veterinary journal (London, England : 1997)    February 25, 2026   Volume 317 106614 doi: 10.1016/j.tvjl.2026.106614
Keller J, Hassenstein MJ, Jung K, Geburek F.Ground reaction forces are accepted as gold standard for objective assessment of weightbearing lameness but measurements at the hoof are limited to experimental devices. This study aimed to evaluate whether innovative instrumented hoof boots (IHBs) could detect and monitor lameness in horses during diagnostic anaesthesia. Twenty-six horses referred for lameness examination (15 front limbs, 17 hindlimbs) were equipped with IHBs and body mounted inertial sensors. Data were collected simultaneously before and after diagnostic anaesthesia until objectively considered positive. Recorded IHB data i...
A topographic analysis of skin thickness in horses.
Veterinary research communications    February 25, 2026   Volume 50, Issue 3 170 doi: 10.1007/s11259-026-11124-4
Hmb O, L W, M V, Jm W, Ct F.A systematic study of regional skin thickness in different types of horses has not yet been described. Knowledge about regional skin thickness has long been used in human medicine to optimise wound care and skin grafting outcomes and similar knowledge could prove useful in equine wound management. The objective of the current study was to report the topographical variation in skin thickness of Warmblood (WB) and Coldblood (CB) type horses and to compare different methods for measuring skin thickness. Horses free of skin conditions and euthanised for reasons unrelated to this study were include...
Machine learning-based prediction and quantification of OCD surgery and pedigree effects on racehorse performance.
Veterinary journal (London, England : 1997)    February 21, 2026   Volume 316 106607 doi: 10.1016/j.tvjl.2026.106607
An SJ, Sohn Y, Forbes E, Ryu SH.Osteochondritis dissecans (OCD) is a common developmental orthopedic condition in Thoroughbred racehorses and although arthroscopic surgery is widely used for treatment, its long-term effects on race performance remain unclear. This retrospective study evaluated the effect of OCD surgery on race performance, compared the predictive power of pedigree and management variables and applied interpretable machine learning methods for forecasting race performance outcomes. Data were collected from 75 Thoroughbreds that underwent OCD surgery between 2015 and 2017 and 257 maternal siblings without reco...
Variation of surface properties over ten consecutive days of Chuckwagon racing on a dirt racetrack.
Journal of equine veterinary science    February 21, 2026   Volume 160 105817 doi: 10.1016/j.jevs.2026.105817
Pfau T, Weller R, Peterson ML, Davis BL, Alamo KG, Eamon CM, Chan ZYS.Chuckwagon races are conducted on dedicated tracks with an "infield" used for rodeo activities and a "track" used for racing. Thoroughbred horses (4 per wagon) first navigate a Chuckwagon in a figure eight maneuver around two barrels in the "infield" before galloping around the "track" and finishing the race in the "infield". Objective: Quantify surface properties (and their association with moisture content) for "track" and "infield". We hypothesized that, due to different preparation, property differences would be larger between "track" and "infield" than across consecutive days and surface ...
Effect of feeder style on intake rate of equine concentrates.
Journal of equine veterinary science    February 19, 2026   Volume 159 105811 doi: 10.1016/j.jevs.2026.105811
O'Reilly K, Aldworth-Yang S, Catalano DN.There are many different concentrate feeders available to horse owners, but little data exists on feeder effect of equine intake rate. Rapid consumption ("bolting") is associated with issues such as esophageal obstruction. Objective: The objective of this study was to evaluate three common concentrate feeders on intake rate. We hypothesized horses would have the slowest intake rate when fed from the trough. Methods: Four geldings were fed 0.454 kg of concentrate from a pan on the ground (P), shallow trough (T), or hanging feeder (H). Horses were randomly assigned a first feeder and then rotate...
The neutrophil-to-lymphocyte ratio is associated with disease severity and infectious etiologies of colitis in horses presenting with acute colitis.
American journal of veterinary research    February 18, 2026   1-9 doi: 10.2460/ajvr.25.12.0447
Samuels AN, Collins N, Albrecht M, Cuming R, Reed S, Gomez D, Toribio R.To evaluate associations between the neutrophil-to-lymphocyte ratio (NLR), nonsurvival, illness severity, and infectious etiologies. Unassigned: This was a retrospective multicenter study that included 100 healthy horses and 444 horses presenting with acute diarrhea to 5 equine referral hospitals. Inclusion criteria were diarrhea duration < 48 hours, complete hemograms, and outcome data. Illness severity was assessed using systemic inflammatory response syndrome. The results of pathogen testing for Salmonella spp, equine coronavirus, Clostridium spp, and Neorickettsia risticii/findlayensis ...
Equine dental destructive disorders: an epidemiological survey in northern Germany.
Frontiers in veterinary science    February 17, 2026   Volume 13 1706621 doi: 10.3389/fvets.2026.1706621
Häussling MPP, Steinberg T, Büttner K, Hannig C, Hannig M, Lemke L, Zierau O, Staszyk C.Destructive lesions of the peripheral and infundibular cementum are increasingly recognized in equine dentistry. While similarities to human caries have been noted, current evidence does not conclusively support this classification. This study aims to assess the prevalence, distribution, and potential risk factors associated with these lesions in a defined horse population. Unassigned: A total of 114 horses from northern Germany underwent standardized oral examination between 2021 and 2023. Clinical data on peripheral and infundibular lesions, diastemata, and influencing factors such as age an...
Chasing safety: Race-day falls and injuries in Irish professional and amateur horseracing from 2016 to 2024.
Journal of science and medicine in sport    February 16, 2026   Volume 29, Issue 9 975-981 doi: 10.1016/j.jsams.2026.02.007
Reville A, Corrigan J, Pugh J, O'Connor S.This study aimed to: 1) examine fall and injury incidence in Irish horseracing between 2016 and 2024, 2) establish injury characteristics, 3) compare incidence rates between professional and amateur racing and 4) investigate changes in incidence rates over time. Methods: Descriptive epidemiology study. Methods: Fall and injury data was recorded by Irish Horseracing Regulatory Board (IHRB)-appointed medical doctors using a standardised injury report form at every Irish race meeting between 2016 and 2024. Fall and injury incidence, injury location and type of injury were reported with 95% Confid...
Thoroughbred Geldings’ Career: Influence of Age at the Start of Training and Racing.
Animals : an open access journal from MDPI    February 12, 2026   Volume 16, Issue 4 576 doi: 10.3390/ani16040576
Hein M, Volkmann N, Probst J, Kemper N, Venner M.This retrospective report investigated whether starting racehorses' training and racing early is associated with a less successful and a shorter racing career. The data of 600 Thoroughbred racehorses from the German Racehorse Association's archiving programme were evaluated. The horses were classified into three groups regarding their different ages when starting training and racing: early training (16-24 months old)/early racing (two years old); early training/late racing (>two years old); and late training (25-30 months). Statistical models investigated effects on the horses' length of ca...
Comparison of two doses of lidocaine constant rate infusion on cardiopulmonary function and recovery in isoflurane-anesthetized horses.
Veterinary anaesthesia and analgesia    February 11, 2026   Volume 53, Issue 3 101202 doi: 10.1016/j.vaa.2026.101202
Henderson AR, Valverde A, Côté N, Sanchez A, Arroyo LG, Gu Y, Johnson R.To compare cardiopulmonary variables and recovery scores in isoflurane-anesthetized horses undergoing arthroscopy using two different intravenous (IV) lidocaine constant rate infusions (CRIs; 50 or 100 μg kg minute) under intermittent positive pressure ventilation (IPPV) or spontaneous ventilation (SV). Methods: Prospective, randomized, clinical study. Methods: A group of 30 client-owned horses, 8-156 months old (range), with body masses of 280-550 kg. Methods: Anesthesia protocol consisted of xylazine, ketamine and diazepam, and maintenance with isoflurane and a CRI of dexmedetomidine. All g...
Sex-Related Differences in Show-Jumping Performance of Retired Thoroughbred Racehorses in Relation to the Interval Since Race Retirement.
Animals : an open access journal from MDPI    February 11, 2026   Volume 16, Issue 4 562 doi: 10.3390/ani16040562
Naito M, Nishihata S, Amano T.To investigate the factors affecting the utilization of retired Thoroughbred racehorses in equestrian disciplines, Bayesian linear mixed models were separately fitted using rank, round time, and obstacle faults from show-jumping competitions restricted to retired Thoroughbred racehorses as dependent variables, with the interaction between horse sex and the interval from race retirement to competition (as a proxy for transition training to show-jumping) as a fixed effect. When the interval was short (≤1 year), the estimated marginal mean of rank was statistically significantly lower in stalli...
In-Depth Analysis of the Prognostic Factors Associated with Short-Term Outcome in Equine Colic Patients: Multicentric Retrospective Study.
Animals : an open access journal from MDPI    February 5, 2026   Volume 16, Issue 3 496 doi: 10.3390/ani16030496
Nocera I, Cingottini D, Di Franco C, Sala G, Bindi F, Spadari A, Rinnovati R, Vitale V, Jose-Cunilleras E, Sgorbini M.Several studies investigated risk and prognostic parameters for horses with colic; however, the consensus is still debated. The present work aimed to investigate colic outcomes and to identify risk factors in horses referred for colic. In this multicenter retrospective study, 236 clinical records of equids referred for colic at three different equine centers were reviewed. The following data were collected: history, signalment, physical examination at the time of admission, hematological and biochemical analysis, diagnosis, SIRS status and 0-6 point-scale SIRS score, colic type, treatment atte...
Space-use by feral cattle and horses shapes vegetation structure in a trophic rewilding area.
Ecological applications : a publication of the Ecological Society of America    February 4, 2026   Volume 36, Issue 1 e70170 doi: 10.1002/eap.70170
Kristensen JÅ, Buitenwerf R, Berti E, Hansen OLP, Schowanek SD, Ejrnæs R, Hansen MDD, Olsen K, Normand S, Svenning JC.Feral cattle (Bos taurus) and horses (Equus ferus caballus) are commonly introduced to European rewilding areas to halt vegetation succession and to conserve light-demanding species. Yet, we still do not understand how the habitat preference of animals shapes vegetation structure at the landscape scale. Here, we used spatial preference modeling to understand drivers of space-use based on GPS-collared horses and cattle in a 120-ha rewilding area in Denmark. Using a time series of a satellite-based vegetation productivity index, we tested the ability of animal space-use to explain changes in veg...
Prognostic Significance of Erythrocyte Sedimentation Rate for Survival in Equine Colic.
Animals : an open access journal from MDPI    February 3, 2026   Volume 16, Issue 3 476 doi: 10.3390/ani16030476
Meistro F, Rinnovati R, Blanc E, Berni P, Napoli S, Marcucci E, D'Angelo P, Ruggeri M, Spadari A, Gialletti R.Accurate early prognostic assessment is a central aspect of clinical decision-making in horses presenting with colic. Despite the availability of multiple clinical and laboratory parameters, reliable biomarkers that provide useful information at the time of admission remain limited. The erythrocyte sedimentation rate is a non-specific indicator of systemic inflammation that has recently been validated for automated point-of-care use in horses. Its behaviour in equine colic has not been previously characterised. This study included 85 horses admitted for colic at two university referral hospita...
Relationship Between Mean Faecal Gastrointestinal Nematode Egg Excretion in Horses and Its Variability: Implications for Control.
Pathogens (Basel, Switzerland)    February 2, 2026   Volume 15, Issue 2 156 doi: 10.3390/pathogens15020156
Cabaret J, Guerrero Molina C, Martínez-Ortiz-de Montellano C, Alcala Canto Y.Faecal egg counts (FECs) are used to assess the intensity of gastrointestinal nematode (GIN) infections in herbivores. FEC distribution is aggregated, meaning that approximately 20% of animals harbour 80% of infections. In times of escalating anthelmintic resistance, it may be necessary to restrict treatment to the animals with the heaviest infections. This strategy is called targeted selective treatment (TST) and is relevant to GIN, for example. The difficulty lies in identifying which animals to treat. One solution is to select potentially at-risk animals based on age (for example, treating ...
Ultrasonographic Evidence of Synovitis Correlates with Synovial Citrate and TBARS in Equine Osteoarthritis.
Veterinary sciences    January 31, 2026   Volume 13, Issue 2 140 doi: 10.3390/vetsci13020140
Barreira AP, Moreira T, Silva R, Nunes L, Lioi A, Kraus E, Altheman V, Ribeiro M, Leite C, Silva A, Almeida F, Santos Junior G, Lessa D, Alves AL.Osteoarthritis (OA) is a degenerative joint disease that affects humans and animals worldwide. Its early diagnosis remains challenging due to subtle clinical signs and late radiographic changes. This study aimed to explore candidate biomarkers associated with spontaneous OA and to investigate their correlation with ultrasonographic scores to support early diagnosis. Clinical, radiographic, and ultrasonographic evaluations were performed on 52 equine metacarpophalangeal joints, with and without OA, allowing joint scoring and classification into osteoarthritis (OAG) and control groups. Synovial ...
Biometric evaluation of different limb regions in Iranian Arab horses.
Open veterinary journal    January 31, 2026   Volume 16, Issue 1 536-549 doi: 10.5455/OVJ.2026.v16.i1.60
Shojaei B, Mousanejad E, Afshar FK, Pourjafari F.Morphological variation in equine limb dimensions provides valuable insight into breed-specific characteristics, with biometric parameters serving as reliable tools for such evaluations. Unassigned: This study aimed to provide a quantitative characterization of the external morphology of the limbs in Iranian Arab horses. Unassigned: Morphometric data were collected from 30 adult specimens of this breed, encompassing key anatomical measurements: scapula length, arm length, forearm length, metacarpus length, forepastern length, metacarpus circumference, femur length, gaskin length, metatarsus le...
Effects of a rehabilitative whole-body resistance band wrap on equine gait, posture, cortisol, and muscular function.
Frontiers in veterinary science    January 28, 2026   Volume 12 1738766 doi: 10.3389/fvets.2025.1738766
Boger B, Naraian M, Hernandez E, Eaton A, Rockburn R, Tillman I, Payne S, Yob C, Panek C, Manfredi JM.Resistance bands used while horses are exercised with their handlers have shown benefits, but it is unknown if whole-body resistance bands used independently have therapeutic benefits. This study hypothesized that horses with varying gait asymmetries would experience improvements in lameness, muscular function, range of motion, posture, and cortisol following short-term use of a whole-body resistance band wrap (RBW). In this study, nine lame adult horses were evaluated with and without the RBW. The assessment included: objective gait analysis, acoustic myography, postural analysis, gait kinema...
Heritability Estimates of Traits Assessed in Field Performance Tests of Polish Warmblood Mares.
Genes    January 28, 2026   Volume 17, Issue 2 148 doi: 10.3390/genes17020148
Lewczuk D, Borowska A, Maśko M, Bagnicka E.: Knowledge of the genetic background of evaluated traits has been the basis for genetic progress in every horse-breeding population and is essential for precise breeding and up-to-date decision-making. The study aimed to estimate the heritability coefficients for field performance traits in mares. : The research was based on 1408 evaluations of mares conducted during the years 2002-2021 in 51 training centers in Poland. The preliminary analyses of the effects, significant for the investigated traits, were obtained using analysis of variance, and these additional data are also presented (SAS p...
Development of an Explainable Machine Learning Computational Model for the Prediction of Severe Complications After Orchiectomy in Stallions.
Animals : an open access journal from MDPI    January 25, 2026   Volume 16, Issue 3 377 doi: 10.3390/ani16030377
Tyrnenopoulou P, Kalatzis D, Kiouvrekis Y, Flouraki E, Folias L, Loukopoulos E, Starras A, Chalvatzis P, Tsioli V, Mavrogianni VS, Fthenakis GC.The objective of the present study was to apply supervised Machine Learning to predict severe complications after equine orchiectomy. A dataset of 612 cases of orchiectomies in stallions was used for the development of a computational model, among which in 8.5% of cases severe complications (colic, continued stallion-like behaviour, evisceration, funiculitis, haemorrhage, and scrotal infection) were diagnosed post-orchiectomy. Three supervised Machine Learning tools were employed: Logistic Regression (12 different models evaluated), Random Forest (64 models), and Gradient Boosting (8 models). ...
Spatial strategies in non-territorial societies: how feral horses maintain boundaries with other groups.
Proceedings. Biological sciences    January 21, 2026   Volume 293, Issue 2063 20252468 doi: 10.1098/rspb.2025.2468
Maeda T, Inoue S, Ringhofer M, Hirata S, Yamamoto S.Encounters between competitive individuals or groups are common in social animals and can involve costly aggression; thus, animals often employ strategies to minimize direct conflict. However, research on whether and how animals adjust their group spatial structure when they encounter or spatially co-occur with a different group remains limited. We investigated how non-territorial units in feral horse multilevel societies manage spatial encounters with neighbouring units. We observed 25 reproductive units in northern Portugal, using drones, and employed statistical analyses to quantify spatial...
Evaluation of oxidative stress in the newborn foal: a multibiomarker approach.
Journal of equine veterinary science    January 20, 2026   Volume 158 105786 doi: 10.1016/j.jevs.2026.105786
Bindi F, Cingottini D, Sorvillo B, Sala G, De Marchi L, Monni G, Celardo S, Bonelli F, Sgorbini M.During the perinatal period, neonates experience a sudden increase in oxygen exposure, predisposing them to oxidative stress. Objective: This prospective, in vivo study aimed to evaluate biomarkers of oxidative stress and antioxidant defence in healthy neonatal foals during the first 48 hours of life. Methods: Ten Standardbred foals were enrolled. Blood samples were collected at birth, 24 hours, and 48 hours after birth and analyzed for biomarkers using spectrophotometry assays. Statistical analysis was performed using non-parametric tests and linear regression. Results: The results revealed s...
Prevalence and risk factors of stress urinary incontinence among female horseback riders in Poland.
Scientific reports    January 17, 2026   Volume 16, Issue 1 5606 doi: 10.1038/s41598-026-36444-y
Zalewski M, Kołodyńska G, Piątek A, Mucha A, Misztela W, Andrzejewski W.Stress urinary incontinence (SUI) is usually associated with aging and parity, but repetitive intra-abdominal pressure during physical activity can also trigger its onset in young women. Horseback riding, despite potential benefits for pelvic floor strengthening, may also contribute to pelvic floor strain and urinary symptoms. The study included 100 Polish women aged 21-54 engaged in horse riding, both recreational (85%) and professional (15%). Participants were divided into three age groups and screened for urinary incontinence symptoms. Quality of life was assessed with validated questionnai...
Herding as an emergent behaviour in harem groups of feral Garrano ponies.
Journal of the Royal Society, Interface    January 15, 2026   Volume 22, Issue 233 20250187 doi: 10.1098/rsif.2025.0187
Africa DD, Ikeda K, Maeda T, Inoue S, Ringhofer M, Yamamoto S, Go CK.Collective decision-making and movement coordination are essential behaviours observed in biological systems, from animal herds to human crowds. Horses are a highly social species with a multilevel society. Herding, where the harem is collected to move in a certain direction, is an often-cited example of agonistic behaviour in horses, yet poorly understood in a granular, quantitative sense. We use transfer entropy to measure herding in a harem group of feral Garrano ponies in Serra D'Arga, Portugal. First, we characterize the harem's leader-follower relationships by quantifying the time lag (a...
Effect of horse sleep behavior on performance in a field-side spatial reversal learning test.
Scientific reports    January 6, 2026   Volume 16, Issue 1 4265 doi: 10.1038/s41598-025-34463-9
Hämäläinen MJ, Brotherus IL, Wigren HM, Kaimio TE, Suomala H, Olbricht AM, Hänninen LT, Mykkänen AK.Reduced rapid-eye-movement (REM) sleep is associated with impaired learning in many species. We developed a reversal learning test (RLT) suitable for field conditions to explore this association in 16 healthy horses. Nocturnal REM-like sleep behavior was recorded five times for 48 h over a six-week period. The horses performed a target training task followed by an RLT using two objects. When the horses reached a predefined frequency of touching the object, the spatial location was reversed. Mean test parameters were statistically analysed using GENLIN models, longitudinal continuous variables...
Genetic and phenotypic insights into thermal load and racing performance of Quarter Horses.
Journal of thermal biology    December 29, 2025   Volume 136 104362 doi: 10.1016/j.jtherbio.2025.104362
Santana ML, Bignardi AB, Faria RAS, Silva JAIV.Thermal load is a major welfare and performance concern for athletic horses, as physical exertion increases internal heat production. Although several phenotypic strategies have been developed to mitigate its effects, the genetic basis of thermal tolerance in horses remains largely unexplored. This study aimed to (1) evaluate the influence of thermal indices on the racing performance of Brazilian Quarter Horses, and (2) investigate the genetic basis of thermal tolerance through the estimation of genetic parameters across temperature gradients. We analyzed 21,857 racing-time (RT) records from 5...
Establishing Reference Values for Serum Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Turkmen Horses: Effects of Gender, Age, Pregnancy and Physical Activity.
Veterinary medicine and science    December 29, 2025   Volume 12, Issue 1 e70755 doi: 10.1002/vms3.70755
Eskandari G, Ahmadi Sheikhsarmast S, Ghasemi S, Mohri M.Neutrophil gelatinase-associated lipocalin (NGAL) is a glycoprotein involved in immune responses and inflammation, with established diagnostic utility in acute kidney injury (AKI) and systemic diseases. However, reference values for NGAL in healthy horses, particularly the Turkmen breed, remain poorly defined. Objective: This study aimed to establish reference values for serum NGAL concentrations in healthy Turkmen horses and to evaluate the influence of age, sex, pregnancy status and physical activity on its levels, as well as its correlations with haematological and serum biochemical variabl...
Efficacy of Ozone Bagging Therapy in Equine Chronic Distal Limb Wounds: Clinical Evaluation of Eight Cases.
Veterinary sciences    December 23, 2025   Volume 13, Issue 1 16 doi: 10.3390/vetsci13010016
Repciuc CC, Oros NV, Mureșan ȘMC, Sevastre B, Joaquim JGF, Oana LI.Limb wounds in horses represent a significant therapeutic challenge due to poor vascularization, reduced skin elasticity, and high risk of complications such as exuberant granulation tissue. Conventional treatments sometimes fail to provide satisfactory healing outcomes, leading to prolonged recovery and increased costs. This study aimed to evaluate the efficacy of topical ozone therapy using the bagging method in promoting the epithelialization and contraction of chronic distal limb wounds in horses refractory to conventional management. Eight horses, aged 3-21 years, with chronic wounds aver...
Descriptive network analysis of Ontario, Canada equine competitions: implications for disease control.
BMC veterinary research    December 23, 2025   Volume 22, Issue 1 43 doi: 10.1186/s12917-025-05248-z
Rossi TM, O'Sullivan TL, Greer AL.Competitions are an important source of entertainment and revenue in the horse industry but may contribute to disease introduction and spread. The objectives of this study were to, (i) describe the annual (2016 to 2018) contact networks of Equestrian Canada competitions in Ontario, Canada, and (ii) determine if the networks exhibit characteristics of 'small world' networks. Data on Equestrian Canada registered competitions in the province of Ontario, Canada between 2016 and 2018 were used to create three types of yearly contact networks: competition networks, horse networks, and venue networks...
Assessment of core stability in equestrian riders.
Folia medica Cracoviensia    December 17, 2025   Volume 65, Issue 3 71-83 doi: 10.24425/fmc.2025.156685
Skwierawska Z, Trybulec B, Jagielski P, Wodka-Natkaniec E.Core stability allows to control and properly perform movements of all body. Optimal core stability level depends on deep muscles capacity and neuromuscular control. Poor core stability is a risk factor for injury, especially during physical activity. Objective: The purpose of this study was to examine the differences in core stability between sport and recreational horse riders and people, who don't do any sports. We hypothesized that horse riders demonstrate better core stability performance. Methods: 75 people aged 15-30 (20 recreational horse riders, 20 sport horse riders and 35 non-horse ...
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