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Snake Envenomation.
The Veterinary clinics of North America. Equine practice    September 15, 2023   Volume 40, Issue 1 133-150 doi: 10.1016/j.cveq.2023.08.003
Gilliam LL.Snakebite envenomation (SBE) in horses can have devastating outcomes. Tissue damage, cardiotoxicity, coagulopathy, and neurotoxicity can be concerns with SBE. Understanding the actions of venom components is important in developing a successful treatment plan. Antivenom is the mainstay of treatment. Long-term deleterious effects can occur including cardiac dysfunction and lameness.
Genomic and comparative analysis of the T cell receptor gamma locus in two Equus species.
Frontiers in immunology    September 15, 2023   Volume 14 1264949 doi: 10.3389/fimmu.2023.1264949
The genus is the only extant genus of the Equidae family, which belongs to Perissodactyla, an order of mammals characterized by an odd number of toes (odd-toes ungulates). Taking advantage of the latest release of the genome assembly, we studied, for the first time in two organisms belonging to the genus, the horse () and the donkey (), the T cell receptor gamma (TRG) locus encoding the gamma chain of the γδ T cell receptor. Forty-five Variable (TRGV) genes belonging to the seven IMGT-NC validated mammalian TRGV subgroups, 25 Joining (TRGJ) and 17 Constant (TRGC) genes organized in 17 V-J-...
Evaluation of the hoof centre-of-pressure path in horses affected by chronic osteoarthritic pain.
PloS one    September 15, 2023   Volume 18, Issue 9 e0291630 doi: 10.1371/journal.pone.0291630
Buser LI, Torelli N, Andreis S, Witte S, Spadavecchia C.The Centre of Pressure (COP) is the single point summarising all forces transferred to the hoof during the stance phase of a stride. COP path (COPp) is the trajectory that COP follows from footstrike to lift-off. Aim of the present study was to characterize the COP and COPp in horses affected by osteoarthritis and chronic lameness. Seventeen adult horses with a diagnosis of osteoarthritis and single limb chronic lameness were recruited. The COP was recorded using a wireless pressure measuring system (TekScan®) with sensors taped to the hooves (either fore- or hind limb, depending on lameness ...
Effect of different manual puncture methods on donkey embryo before vitrification.
Theriogenology    September 15, 2023   Volume 214 134-140 doi: 10.1016/j.theriogenology.2023.09.010
Li N, Dai S, Wu H, Zhang F, Song S, Guo Y, Wang S, Chang S, Zeng S.The application of embryo recovery and transfer technology in the donkey industry is far lower than that of horses and cattle. Sometimes the recovered embryos could not be transferred in time, which required embryo cryopreservation. The embryo cryopreservation technology is more conducive to the preservation and transportation of recovered embryos with excellent genetic traits. However, this technique for donkey embryos is not efficient and needs further optimization. The objective of this study was to evaluate the effect of different manual puncture methods on the viability and pregnancy rate...
Severely Asthmatic Horses Residing in a Mediterranean Climate Shed a Significantly Lower Number of Parasite Eggs Compared to Healthy Farm Mates.
Animals : an open access journal from MDPI    September 15, 2023   Volume 13, Issue 18 2928 doi: 10.3390/ani13182928
Simões J, Sales Luís JP, Madeira de Carvalho L, Tilley P.The relationship between helminth infection and allergic diseases has long intrigued the scientific community. This interaction was previously studied in a horse family with high incidence of severe equine asthma and in non-related severely asthmatic horses from equine hospital referrals in Switzerland. Our aim was to determine if this interaction would also be observed in a group of non-related client-owned severely asthmatic horses living in a Mediterranean climate and recruited through a first-opinion veterinarian group. Fecal samples from severe equine asthma-affected and healthy horses li...
Strongyle faecal egg counts in Swiss horses: A retrospective analysis after the introduction of a selective treatment strategy.
Veterinary parasitology    September 14, 2023   Volume 323 110027 doi: 10.1016/j.vetpar.2023.110027
Lüthin S, Zollinger A, Basso W, Bisig M, Caspari N, Eng V, Frey CF, Grimm F, Igel P, Lüthi S, Regli W, Roelfstra L, Rosskopf M, Steiner B....The standard parasite management of horses based on regular anthelmintic treatments, now practiced for decades has resulted in a worrying expansion of resistant helminth populations, which may considerably impair control on the farm level. The aim of the present study was to obtain a retrospective (year 2010 - 2016) nationwide analysis of faecal egg count (FEC) data from the Swiss adult horse population, related to horse age and geographic region. Thirteen labs provided a total of 16,387 FEC data of horses aged four to 39 years (average: 13.6 years). The annual number of performed FEC tests i...
Distribution of equine and food animal emergency caseload by time of day, day of week, time of year, holiday, and phase of moon at an academic ambulatory and referral hospital.
Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)    September 14, 2023   Volume 33, Issue 6 696-704 doi: 10.1111/vec.13334
Graves MT, Zhu X.Determine the association of time of day, day of week, time of year, holiday, and phase of moon on academic ambulatory and hospital emergency caseload for equine and food animal species. Methods: Retrospective study. Methods: Large animal teaching hospital emergency service. Methods: Equine and food animals. Methods: None. Results: The hospital database was searched for after-hours on-farm and in-hospital emergencies seen from 2014 to 2020. Variables included date and time of admission, species (equine or food/fiber animal [FA]), and hospital service (field or in-hospital). The association of ...
Parascaris spp. eggs shedding patterns in juvenile horses.
Veterinary parasitology    September 14, 2023   Volume 322 110029 doi: 10.1016/j.vetpar.2023.110029
Ripley NE, Gravatte HS, Britton LN, Davis SM, Perrin GM, Warner S, Rexroat EK, Vetter AL, Maron EES, Finnerty CA, Stanton V, Nielsen MK.Parascaris spp. infect foals worldwide and foals typically shed eggs in the feces from about three to six months of age, upon which natural immunity is incurred. High levels of anthelmintic resistance of Parascaris spp. are a global concern, and further understanding egg shedding patterns and fecal egg counting (FEC) data variability is of high importance. The aims of this study were to monitor Parascaris spp. egg shedding in untreated foals during 12-23 weeks of age, estimate sources of data variability, and assess precision of two ascarid FEC techniques. Fecal samples were collected weekly f...
Kinetic, genomic, and physiological analysis reveals diversity in the ecological adaptation and metabolic potential of Brachybacterium equifaecis sp. nov. isolated from horse feces.
Microbiology spectrum    September 14, 2023   Volume 11, Issue 5 e0504822 doi: 10.1128/spectrum.05048-22
Farooq A, Lee M, Han S, Jung GY, Kim SJ, Jung MY.Brachybacterium species have been identified in various ecological niches and belong to the family within the phylum . In this study, we isolated a novel JHP9 strain from horse feces and compared its kinetic, biochemical, and genomic features with those of other strains. Moreover, comparative genomic analysis using publicly available genomes was performed to determine the properties involved in their ecological adaptation and metabolic potential. Novel species delineation was determined phylogenetically through 16S rRNA gene similarity (up to 97.9%), average nucleotide identity (79.5-82.5%...
Evaluation of domestic animal sperm head morphology via flow cytometric DNA labelling and pulse shape analysis using bull and stallion spermatozoa as model species.
Reproduction in domestic animals = Zuchthygiene    September 14, 2023   Volume 58, Issue 11 1569-1575 doi: 10.1111/rda.14472
Nagy S, Kovacs B, Johannisson A.The aim of the present study was to test a rapid, robust flow cytometric technique for the detection of sperm head abnormalities of domestic bulls and stallions. The so-called PulSA approach detects the pulse profiles of propidium-iodide labelled spermatozoa. In the first experiment, species-specific threshold values were established on sperm samples that were tested for sperm head abnormalities with a classic visual morphology analysis. In the second experiment, serial mixtures of bull and stallion spermatozoa mimicking different percentages of sperm head abnormalities were analysed. Non-metr...
Three-Dimensional Imaging and Histopathological Features of Third Metacarpal/Tarsal Parasagittal Groove and Proximal Phalanx Sagittal Groove Fissures in Thoroughbred Horses.
Animals : an open access journal from MDPI    September 14, 2023   Volume 13, Issue 18 2912 doi: 10.3390/ani13182912
Lin ST, Foote AK, Bolas NM, Peter VG, Pokora R, Patrick H, Sargan DR, Murray RC.Fissure in the third metacarpal/tarsal parasagittal groove and proximal phalanx sagittal groove is a potential prodromal pathology of fracture; therefore, early identification and characterisation of fissures using non-invasive imaging could be of clinical value. Thirty-three equine cadaver limbs underwent standing cone-beam (CB) computed tomography (CT), fan-beam (FB) CT, low-field magnetic resonance imaging (MRI), and macro/histo-pathological examination. Imaging diagnoses of fissures were compared to microscopic examination. Imaging features of fissures were described. Histopathological fin...
Recombinant Salmonella enterica OmpX protein expression and its potential for serologically diagnosing Salmonella abortion in mares.
Veterinary world    September 13, 2023   Volume 16, Issue 9 1790-1795 doi: 10.14202/vetworld.2023.1790-1795
Borovikov S, Ryskeldina A, Tursunov K, Syzdykova A, Akibekov O. abortion in mares is caused by subspecies serovar infection and is characterized by premature (abortion) or non-viable fetus birth. Although all horses are susceptible to infection, the condition is more often clinically manifested in pregnant mares, with most abortions recorded in young females. In addition, nonspecific clinical disease signs and poorly sensitive and effective bacteriological diagnostic methods hinder rapid and reliable infection diagnoses. Immunochemical methods such as enzyme-linked immunosorbent assay (ELISA) and immunochromatography assays can facilitate effective and...
Intra-articular triamcinolone acetonide injection results in increases in systemic insulin and glucose concentrations in horses without insulin dysregulation.
Equine veterinary journal    September 13, 2023   doi: 10.1111/evj.14003
Boger BL, Manfredi JM, Loucks AR, Salamey MZ, Kapeller LE, Fricano AG, Winkler A, Yob C, Colbath AC.Corticosteroids are a commonly used, inexpensive intra-articular treatment for osteoarthritis which may increase the risk for laminitis in horses due, in part, to hyperinsulinaemia. Humans with metabolic syndrome experience increases in insulin and glucose concentrations post-injection, but responses in horses are unknown. Objective: To determine the effect of a single intra-articular (IA) dose of triamcinolone acetate (TA) on blood insulin and glucose concentrations. Methods: Before-after study. Methods: Ten horses with normal insulin regulation as assessed by an oral sugar test received 18â€...
Associations between feeding and glucagon-like peptide-2 in healthy ponies.
Equine veterinary journal    September 13, 2023   doi: 10.1111/evj.14004
Sibthorpe PEM, Fitzgerald DM, Sillence MN, de Laat MA.Gastrointestinal peptides, such as glucagon-like peptide-2 (GLP-2), could play a direct role in the development of equine hyperinsulinaemia. Objective: To describe the secretory pattern of endogenous GLP-2 over 24 h in healthy ponies and determine whether oral administration of a synthetic GLP-2 peptide increases blood glucose or insulin responses to feeding. Methods: A cohort study followed by a randomised, controlled, cross-over study. Methods: In the cohort study, blood samples were collected every 2 h for 24 h in seven healthy ponies and plasma [GLP-2] was measured. In the cross-over...
Computed tomographic imaging and surgical management of distal insertional avulsion fragments of the caudal cruciate ligament in four horses.
Veterinary surgery : VS    September 13, 2023   Volume 52, Issue 8 1228-1236 doi: 10.1111/vsu.14025
Bolz NM, Ehrle A, Mählmann K, Lischer CJ.To describe cases with caudal cruciate ligament (CdCL) avulsion fragments diagnosed based on computed tomography (CT) examination and report on arthroscopic fragment removal. Methods: Four Warmblood horses with hindlimb lameness and osseous fragments located in the caudal medial femorotibial joint (mFTJ). Methods: Short case series. Methods: CT and arthroscopic evaluation of the caudal mFTJ were performed. The caudal mFTJ and the insertion of the CdCL on the tibia were assessed and removal of the avulsion fragments was attempted in three horses using a cranial intercondylar approach. Results: ...
Development of multiplex gold nanoparticles biosensors for ultrasensitive detection and genotyping of equine herpes viruses.
Scientific reports    September 13, 2023   Volume 13, Issue 1 15140 doi: 10.1038/s41598-023-41918-4
Ghoniem SM, ElZorkany HE, Hagag NM, El-Deeb AH, Shahein MA, Hussein HA.Gold nanoparticles (GNPs) biosensors can detect low viral loads and differentiate between viruses types, enabling early diagnosis and effective disease management. In the present study, we developed GNPs biosensors with two different capping agent, citrate-GNPs biosensors and polyvinylpyrrolidone (PVP)-GNPs biosensors for detection of EHV-1 and EHV-4 in multiplex real time PCR (rPCR). Citrate-GNPs and PVP-GNPs biosensors can detect dilution 10 of EHV-1 with mean Cycle threshold (Ct) 11.7 and 9.6, respectively and one copy as limit of detection, while citrate-GNPs and PVP-GNPs biosensors can de...
Detection of fungi in the airways of horses according to the sample site: a methodological study.
Veterinary research communications    September 13, 2023   doi: 10.1007/s11259-023-10213-y
Lemonnier LC, Couroucé A, Cessans M, Petit L, Cardwell JM, Barbazanges P, Toquet M-, Richard EA.Fungal detection in equine airways may be performed on either tracheal wash (TW) or bronchoalveolar lavage fluid (BALF) by either cytology or culture. However, method comparisons are sparse. Our objective was to determine the prevalence of fungi in airways of horses according to the sample site and laboratory methodology. Sixty-two adult horses, investigated in the field or referred for respiratory disease, were included. Tracheal wash, and BALF collected separately from both lungs, were collected using a videoendoscope. Fungi were detected in cytologic samples examined by light microscopy, an...
Unravelling of the genetic diversity, population structure, and phylogenetic relationships of the indigenous horse breeds of Pakistan.
Gene    September 13, 2023   Volume 889 147736 doi: 10.1016/j.gene.2023.147736
Sohail Ashraf M, Basheer A, Iqbal F, Zahoor I.The current study aimed to evaluate the genetic diversity, level of admixture, and phylogenetic relationship, of the Pakistani horse breeds, along with their morphological characterization. Data for the body measurements showed that Morna horses had the highest values of body height, body length, chest girth, leg length, and head length, whereas the Baluchi horses had the lowest values for these traits. For the genetic diversity 64 animals, 15/breed except for Baluchi(14) and Topras(05), were genotyped by using the 17-plex equine genotyping kit. The AMOVA results showed that 13% of genetic div...
Xylazine Infusion during Equine Colic Anesthesia with Isoflurane and Lidocaine: A Retrospective Study.
Animals : an open access journal from MDPI    September 13, 2023   Volume 13, Issue 18 doi: 10.3390/ani13182902
Ruíz-López P, Cuypers C, Schauvliege S.This retrospective study investigated the effect of a xylazine infusion on heart rate; mean arterial pressure; blood gases; anesthetic and dobutamine requirements; recovery quality and duration; percentage of death/survival; and days to die/discharge in horses after colic surgery under partial intravenous anesthesia with isoflurane and lidocaine infusion. Anesthetic records of equine colic surgery were reviewed from similar periods in 2020-2021 and 2021-2022. In both groups, after sedation with xylazine 0.7 mg/kg intravenously (IV) and induction with ketamine 2.2 mg/kg and midazolam 0.06 mg/kg...
Detection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathy.
Equine veterinary journal    September 12, 2023   doi: 10.1111/evj.14007
Velloso Alvarez A, Jose-Cunilleras E, Dorrego-Rodriguez A, Santiago-Llorente I, de la Cuesta-Torrado M, Troya-Portillo L, Rivera B, Vitale V....Real-time PCR is the diagnostic technique of choice for the diagnosis and control of equine herpesvirus-1 (EHV-1) in an outbreak setting. The presence of EHV-1 in nasal swabs (NS), whole blood, brain and spinal cord samples has been extensively described; however, there are no reports on the excretion of EHV-1 in urine, its DNA detection patterns, and the role of urine in viral spread during an outbreak. Objective: To determine the presence of EHV-1 DNA in urine during natural infection and to compare the DNA detection patterns of EHV-1 in urine, buffy coat (BC) and NS. Methods: Descriptive st...
Evaluating the efficacy of hippotherapy: a promising intervention in rheumatology, pain medicine, and geriatrics.
Rheumatology international    September 12, 2023   Volume 43, Issue 12 2185-2191 doi: 10.1007/s00296-023-05451-x
Using animals for medical care has a long history. Animals have long been recognized for their ability to improve human health and well-being throughout nations and civilizations. Animal-assisted therapy is a concept that incorporates intentional and regulated interactions between individuals and animals to achieve therapeutic aims. Hippotherapy is an animal-assisted therapy consisting of medical procedures and practices that use horses to restore and enhance physical and mental health. The effectiveness of hippotherapy is evidenced by its ability to have a favorable influence on various dom...
A de novo 2.3 kb structural variant in MITF explains a novel splashed white phenotype in a Thoroughbred family.
Animal genetics    September 12, 2023   Volume 54, Issue 6 752-762 doi: 10.1111/age.13352
Bellone RR, Tanaka J, Esdaile E, Sutton RB, Payette F, Leduc L, Till BJ, Abdel-Ghaffar AK, Hammond M, Magdesian KG.Splashed white in horses is characterized by extensive white patterning on the legs, face and abdomen and may be accompanied by deafness. To date, seven variants in microphthalmia-associated transcription factor (MITF) and two variants in Paired Box 3 (PAX3) have been identified to explain this phenotype. A splashed white Thoroughbred stallion, whose sire and dam were not patterned, was hypothesized to have a de novo variant leading to his white coat pattern. A whole-genome sequencing candidate gene approach identified two single nucleotide variants (SNVs) in SOX10, four SNVs in MITF and a 2.3...
Factors associated with safe completion of Fédération Equestre Internationale eventing cross-country (2008-2018).
Equine veterinary journal    September 12, 2023   Volume 56, Issue 5 902-913 doi: 10.1111/evj.14002
Bennet ED, Cameron-Whytock H, Parkin TDH.Equestrian eventing involves competing in three phases: dressage, jumping, and cross-country. Competitors are ranked by number of penalties accrued-with those who have fewer penalties ranked higher. Completing the cross-country phase with zero obstacle penalties is commonly referred to as 'running clear'. Understanding factors associated with running clear can help athletes plan strategically for success, while also helping governing bodies to refine qualification criteria for elite levels. Objective: This study was carried out to identify factors associated with running clear in the cross-cou...
Analysis of the Usage Context of an mHealth Application for Equestrians.
Studies in health technology and informatics    September 12, 2023   Volume 307 117-125 doi: 10.3233/SHTI230702
Haase L.One possibility to support veterinarians in times of a vet shortage is by providing animal owners with a technical decision support for deciding whether their animal needs to be seen by a vet. As the first step in the user-centered development of such an mHealth application for equestrians, an analysis of the context of use was done. Methods: The analysis was carried out by reviewing existing literature and conducting an online survey with 100 participants. Results: Characteristics of the user group and the usage context are presented using an adaptation of the four layers of diversity. Many e...
Digitalisation in pre-purchase examination and prospects for an equine health database in Germany-Results of an online survey among equine veterinarians.
Equine veterinary journal    September 12, 2023   doi: 10.1111/evj.14001
Folgmann MS, Kleinsorgen C, Stock KF, Meister D, Hellige M, Feige K, Delling U.Digital recording and storage of health data are becoming increasingly important in the fields of both human and veterinary medicine. There is currently a lack of information on the level of digitalisation among equine veterinarians in Germany and their attitudes towards advanced digitalisation. Objective: To collect opinions of equine veterinarians about (1) digital data collection in pre-purchase examinations (PPEs), and (2) the establishment of an equine health database in Germany. Methods: Cross-sectional survey. Methods: An online survey was developed and distributed via email exclusively...
Prevalence and Factors Related to Leishmania infantum Infection in Healthy Horses (Equus caballus) from Eastern Spain.
Animals : an open access journal from MDPI    September 11, 2023   Volume 13, Issue 18 2889 doi: 10.3390/ani13182889
Martínez-Sáez L, Dulac Q, Montaner-Angoiti E, Marín-García PJ, Llobat L.Leishmaniosis is a zoonosis caused by spp., an intracellular protozoan parasite. This parasite is transmitted by sandflies, and the disease is endemic in the Mediterranean basin. In recent years, the number of species which could be a reservoir of the parasite is increased. One of the most relevant species is the horse, due to their contact with humans and ability to control the disease, thus being a possible silent reservoir. In this study, we have analyzed the prevalence and factors related to infection in healthy horses in the Mediterranean region. Epidemiological data and serum samples w...
Comprehensive Analysis of Equid Herpesvirus Recombination: An Insight Into the Repeat Regions.
Journal of equine veterinary science    September 11, 2023   Volume 130 104916 doi: 10.1016/j.jevs.2023.104916
Tau RL, Ferreccio C, Bachir N, Torales F, Romera SA, Maidana SS.High-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses with an economic impact on the horse industry, can recombine. This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between...
Discrepancies between phenotypic and genotypic identification methods of antibiotic resistant genes harboring Staphylococcusaureus.
Microbial pathogenesis    September 11, 2023   Volume 184 106342 doi: 10.1016/j.micpath.2023.106342
Rasheed H, Ijaz M, Ahmed A, Javed MU, Shah SFA, Anwaar F.Antimicrobial resistance is a global issue that limits therapeutic options for infections. S. aureus being a member of the ESKAPE group is capable of "escaping" the biocidal action of antimicrobial agents. There are phenotypic and genotypic methods used for the identification of antibiotic resistant genes harboring S. aureus but these methods do not always show concordant results. To address these discrepancies, a total of 335 equine nasal swab samples from four districts of Punjab were collected using a convenient sampling technique. These samples were first subjected to common microbial tech...
Evidence of Horse Exposure to Anaplasma phagocytophilum, Borrelia burgdorferi, and Leishmania infantum in Greece through the Detection of IgG Antibodies in Serum and in an Alternative Diagnostic Sample-The Saliva.
Biomolecules    September 11, 2023   Volume 13, Issue 9 doi: 10.3390/biom13091374
Athanasiou LV, Katsogiannou EG, Tyrnenopoulou P, Gougoulis D, Apostolidis KN, Papadakis SM, Kokkinaki KCG, Papatsiros VG, Tsokana CN.Among the various zoonotic pathogens that infect horses, Anaplasma phagocytophilum, Borrelia spp. and Leishmania spp. have gained scientific interest, and relevant molecular and serological studies in horses have been conducted worldwide. Moreover, human and veterinary medicine have extensively applied alternatives to serum diagnostic samples-such as saliva-for detecting pathogens or antibodies. In this study, we investigated the exposure of horses in Greece to A. phagocytophilum, B. burgdorferi, and L. infantum, and we assessed the diagnostic accuracy of saliva compared to serum in detecting ...
High-level competition exercise and related fatigue are associated with stride and jumping characteristics in eventing horses.
Equine veterinary journal    September 11, 2023   doi: 10.1111/evj.13999
Burger D, Vidondo B, Gerber V, Deillon D, Müller A, Scheidegger M, Käser R, Ramseyer A.Fatigue and related injuries to the musculoskeletal system are among the most frequent reasons for the withdrawal of high-level eventing horses from the sport. The safety of both horse and rider is very important, and early detection of fatigue is crucial. Objective: To investigate elite eventing horses in competitive events focusing on biomechanical, cardiovascular and metabolic variables across the cross-country test and to identify their potential associations with fatigue. Methods: Prospective observational exploratory field study. Methods: Observations on 54 cross-country tests of 33 hors...