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Search 66,193 equine research studies and published journal articles.
Ultra-rapid freezing in spheres yields a higher cryoresistance than in straws but remains inferior to conventional slow freezing of stallion sperm.
Cryobiology    September 8, 2025   Volume 121 105303 doi: 10.1016/j.cryobiol.2025.105303
Larriva-González F, Erráez-Guaicha JE, Torres-Ordóñez KE, Duma M, Larriva-Salazar S, Galarza DA.This study evaluated the cryoresistance of stallion sperm frozen by ultra-rapid (UR) methods using microspheres and straws or by the conventionally-slow (CS) method. Sixteen ejaculates from four stallions were each divided into three aliquots according to the freezing method: UR freezing in 30-μL spheres (UR-Spheres) by direct immersion in liquid nitrogen (LN); UR freezing in 0.25-mL straws (UR-Straws) by direct horizontal submersion in LN; and CS freezing in LN vapors. Ultra-rapid freezing medium included 100 mM trehalose +1 % BSA, and the CS freezing medium contained 5 % dimethylformamid...
Transcriptome Analysis of Muscle Tissue from Three Anatomical Locations in Male and Female Kazakh Horses.
Biology    September 8, 2025   Volume 14, Issue 9 1216 doi: 10.3390/biology14091216
Wubuli A, Su Y, Yao X, Meng J, Wang J, Zeng Y, Li L, Ren W.The Kazakh horse, a versatile breed, is renowned for stable genetic performance and strong tolerance to coarse feed. Sex is a key factor influencing skeletal muscle development. However, the mechanisms underlying sex-specific regulation of equine muscle growth remain obscure. This study employed transcriptomic analysis to investigate sex-associated molecular differences in skeletal muscle of Kazakh horses. The experimental cohort comprised four three-year-old Kazakh stallions and four three-year-old Kazakh mares. After slaughter, six groups of muscle samples were collected immediately, includi...
Daily briefing: A polo-team’s worth of cloned CRISPR horses.
Nature    September 8, 2025   doi: 10.1038/d41586-025-02908-w
Graham F.No abstract available
Temperatures of the Mouthpiece of the Bit of Carriage Horses over a Period of 11 Months.
Animals : an open access journal from MDPI    September 7, 2025   Volume 15, Issue 17 2623 doi: 10.3390/ani15172623
Krcal C, Licka T.Besides oral temperature, meteorological parameters are expected to be relevant for mouthpiece temperature, potentially influenced by the material, surface area and weight of the bit. This study measured the temperature of the mouthpieces at the corner of the mouth while they were in use on 58 carriage horses during the four 2024 seasons. Stainless steel, copper, and copper-steel bits were tested in three shapes: Butterfly Liverpool, Liverpool, and Loose Ring Snaffle with four rings. Additionally, surface temperatures of inner thighs, the ground, and buildings were measured using infrared ther...
Sexual arousal intensity modulates copulatory behavior and semen quality in Arabian stallions: Effects of age, origin, and collection method.
Veterinary world    September 6, 2025   Volume 18, Issue 9 2615-2625 doi: 10.14202/vetworld.2025.2615-2625
Najjar A, Dhaouadi A, Ezzar S, Benaoun B, Khaldi S.Stallion sexual behavior during semen collection can be influenced by multiple factors, yet the role of sexual arousal intensity remains underexplored. Understanding how arousal modulates behavioral and physiological reproductive traits is essential for improving artificial insemination (AI) efficiency and semen quality. This study aimed to evaluate the effects of sexual arousal intensity, age, origin, and semen collection method on stallion sexual behavior and the quality of fresh and frozen semen. Unassigned: Thirteen Arabian stallions (7 Tunisian, 6 foreign parentage) aged 6-20 years (total...
First CRISPR horses spark controversy: what’s next for gene-edited animals?
Nature    September 6, 2025   Volume 645, Issue 8081 565-566 doi: 10.1038/d41586-025-02800-7
Kavanagh K.No abstract available
Technologies for equine welfare and performance monitoring under field conditions – Where do we stand?
Equine veterinary journal    September 6, 2025   doi: 10.1111/evj.70092
Aarts RM, Siegers EW, Serra Braganca FM, van Weeren PR.The need for comprehensive equine welfare assessments has become particularly evident amid ongoing debates about the social licence to operate in equestrian sports. During exercise, multiple physiological systems, principally the cardiovascular, respiratory, muscular, thermoregulatory, endocrine, and locomotory systems, undergo complex adaptations. To monitor and determine equine welfare, an approach that focuses on the quantitative monitoring of both physiological and psychological parameters to determine and understand the impact of equestrian sports on horses is essential. Existing and emer...
Youth horse-related injuries (2014-2024): a scoping review of epidemiological and disciplinary insights.
BMJ open sport & exercise medicine    September 5, 2025   Volume 11, Issue 3 e002589 doi: 10.1136/bmjsem-2025-002589
Boije Af Gennäs K, Jungmalm J.Although horse riding is hazardous and injuries are common, young riders regularly engage in horse-related activities. To our knowledge, there have been no syntheses on youth horse-related injuries published during the past decade that employ a multi- and interdisciplinary research agenda (M-IDR) and that incorporate both quantitative and qualitative methods. Therefore, this scoping review aimed to (1) review studies on horse-related injuries among children and adolescents and (2) identify methodological and paradigmatic trends according to M-IDR. Using the Preferred Reporting Items for System...
Charting the equine miRNA landscape: An integrated pipeline and browser for annotating, quantifying, and visualizing expression.
PLoS genetics    September 5, 2025   Volume 21, Issue 9 e1011835 doi: 10.1371/journal.pgen.1011835
Cullen JN, Cieslak J, Petersen JL, Bellone RR, Finno CJ, Kalbfleisch TS, Calloe K, Capomaccio S, Cappelli K, Coleman SJ, Distl O, Durward-Akhurst SA....MicroRNAs (miRNAs) are essential regulators of gene expression, yet few comprehensive databases exist for miRNA expression in non-model species, limiting our ability to characterize their roles in gene regulation, development, and disease. Similarly, isomiRs - length and sequence isoforms of canonical miRNAs with potentially altered regulatory targets and functions - have received even less attention in non-model species, including the horse, leaving a critical gap in our understanding of their biological significance. To address these challenges, we developed an open-source, containerized pip...
Influenza D Virus Circulation Among Bovines, Swine, Equines, and Wild Boars in Italy: A Sero-Epidemiological Study.
Pathogens (Basel, Switzerland)    September 5, 2025   Volume 14, Issue 9 891 doi: 10.3390/pathogens14090891
Falsini A, Coppola C, Fiori A, Buonavoglia D, Marchi S, Montomoli E, Pellegrini F, Lanave G, Martella V, Camero M, Trombetta CM.Influenza D virus (IDV), belonging to the family, was first discovered in 2011 in pigs. Surveys in humans and animals have been carried out to decipher IDV ecology. In this seroepidemiological study, we investigated the circulation of IDV lineages across Italy in livestock and wildlife animals. A total of 1038 animal serum samples (from 246 bovines, 249 swine, 98 equines, and 445 wild boars) were tested using hemagglutination inhibition and virus neutralization assays. The results confirm bovines as the primary reservoir for IDV, with high seroprevalence for both D/660 (87%) and D/OK (80%) st...
Behavioral Assessment of Equine Relaxation Following Manual Therapy: A Pilot Study.
Veterinary sciences    September 5, 2025   Volume 12, Issue 9 865 doi: 10.3390/vetsci12090865
Paksoy Y, Ural K, ErdoÄŸan H, ErdoÄŸan S, PaÅŸa S.The aim of this pilot study was to evaluate the relaxation, stress reduction and behavioral changes observed after manual therapy applied to horses exposed to racing and physical training stimulus. This descriptive approach is aimed at veterinary clinicians to evaluate the therapy process more effectively with behavioral feedback. For this purpose, the study was conducted in two different equestrian clubs in Adana (Adana Mediterranean and Suvari Equestrian Clubs) between 2023 and 2024. A total of 32 racehorses (16 Thoroughbred, 16 Arabian; 16 female, 16 male) of different ages, genders and bre...
2D-shear wave elastographic features of normal and injured equine superficial digital flexor tendons.
Equine veterinary journal    September 5, 2025   doi: 10.1111/evj.70091
Guerri G, Bandera L, Straticò P, Palozzo A, Di Nunzio L, Celani G, Varasano V, Vignoli M, Petrizzi L.Superficial digital flexor tendon (SDFT) injuries cause progressive loss of its mechanical properties. Two-dimensional shear wave elastography (2D-SWE) provides information about tissue stiffness. Objective: To determine the feasibility, repeatability, and reproducibility of 2D-SWE of healthy and pathological forelimb SDFT. Methods: Prospective observational cohort study. Methods: Horses were divided into a noninjured Group (H) and a Group with tendinopathy of the SDFT (P). 2D-SWE of both fore SDFTs was performed at three metacarpal levels (A, B, and C, from proximal to distal). Qualitative an...
Suzetrigine in Equestrian Sports: Optimized Extraction and LC-HRMS Detection Strategies.
Rapid communications in mass spectrometry : RCM    September 5, 2025   Volume 39, Issue 23 e10135 doi: 10.1002/rcm.10135
Ajeebsanu MM, Koshy SA, Karakka Kal AK, Subhahar MB, Karatt TK, Philip M.Suzetrigine, a recently approved Na1.8 sodium channel blocker, shows strong potential in the treatment of neurological, psychiatric, and pain-related conditions. Its peripheral selectivity enables effective pain management while avoiding central nervous system complications and addiction risks linked to opioid use. Following FDA approval in January 2025, concerns have emerged regarding its possible misuse for performance enhancement in sports, highlighting the need for reliable detection tools in doping control. Methods: An analytical procedure was designed and validated to detect suzetrigine ...
West Nile virus in horses: surveillance, diagnosis and prevention in the UK.
The Veterinary record    September 5, 2025   Volume 197, Issue 5 e5926 doi: 10.1002/vetr.5926
Whitlock F, Newton R, King S, Ionescu AM, Higgins S.With the geographical distribution of West Nile virus expanding, there is growing concern about the potential for an incursion to the UK. Here, Fleur Whitlock, Richard Newton, Simon King, Andra-Maria Ionescu and Sara Higgins provide a timely reminder of key aspects of the virus' biology.
Clinical and ultrasonographic findings in horses with desmopathy of the palmar/plantar abaxial ligaments of the proximal interphalangeal joint: 20 cases.
Journal of the American Veterinary Medical Association    September 5, 2025   Volume 263, Issue 12 1560-1565 doi: 10.2460/javma.25.04.0290
Gutierrez SO, Garcia-Lopez JM, Chope KB, Levine DG, Ortved K, Brown KA.To describe the clinical and ultrasonographic findings in horses with desmopathy of the abaxial palmar/plantar ligament (APL) of the proximal interphalangeal joint and the prevalence of concurrent musculoskeletal injuries in the pastern and foot. Unassigned: Horses presenting to New Bolton Center between March 2017 and November 2022 with an age ranging from 5 to 20 years that underwent lameness evaluation in which lameness was localized to the distal limb and had an ultrasonographic diagnosis of APL desmopathy. Unassigned: Horses included were those that (1) presented for a lameness examinatio...
Comparison Between Tracheal Wash and Bronchoalveolar Lavage Cytology for the Assessment of Airway Inflammation in Racehorses Affected by Exercise-Induced Pulmonary Hemorrhage.
Animals : an open access journal from MDPI    September 5, 2025   Volume 15, Issue 17 2609 doi: 10.3390/ani15172609
Bozzola C, Sala G, Stancari G, Ferrucci F, Zucca E.Exercise-Induced Pulmonary Hemorrhage (EIPH) results from alveolar capillary stress failure after strenuous exercise, leading to bleeding and pulmonary inflammation. Bronchoalveolar lavage fluid (BALF) cytology is considered the gold standard for the assessment of airway inflammation; however, it is often considered more invasive compared to tracheal wash (TW). It has been demonstrated that TW is a reliable diagnostic tool that may substitute BALF to diagnose and grade the severity of EIPH in racehorses; however, no information about airway inflammation in EIPH-affected horses has been reporte...
Effects of mixed polyphenol supplementation on lower airway inflammation in horses with Equine Asthma Syndrome.
Journal of equine veterinary science    September 5, 2025   Volume 154 105681 doi: 10.1016/j.jevs.2025.105681
Andrews KM, Berghaus LJ, Hart KA.Equine Asthma Syndrome (EAS) is a chronic inflammatory respiratory disease of adult horses. While dietary polyphenols have shown anti-inflammatory effects in human asthma, their use in equine asthma has not been studied. Objective: To evaluate the effects of a mixed polyphenol supplement on systemic and pulmonary inflammation, bronchoalveolar lavage (BAL) cytology, and clinical respiratory signs in healthy and asthmatic horses. We hypothesized that polyphenol supplementation would decrease systemic and pulmonary inflammation in both healthy and asthmatic horses and would improve BAL cytology a...
Clinical audit of pre-procedural checklists in an equine referral hospital.
Equine veterinary journal    September 5, 2025   doi: 10.1111/evj.70097
Beeston TJ, Duncan JC, Pollock PJ.Surgical safety checklists have demonstrated a positive impact on post-surgical morbidity/mortality in human medicine, and likely have an equal benefit in veterinary medicine. To realise their advantages, they must be correctly and regularly used. A clinical audit was planned to assess this. Objective: To determine the compliance with the pre-procedural request form/surgical safety checklist in a large multi-disciplinary equine referral hospital. Methods: Retrospective full-cycle clinical audit. Methods: One hundred and forty-eight checklists (consisting of 23 sub-sections) were examined for c...
Commercial cytokine assay on equine cerebrospinal fluid does not distinguish equine degenerative myeloencephalopathy from cervical vertebral stenotic myelopathy.
American journal of veterinary research    September 5, 2025   Volume 86, Issue 12 ajvr.25.06.0212.xml doi: 10.2460/ajvr.25.06.0212
Payette F, Kulp JC, Graves A, Janes J, Morrow J, Orr K, Reed S, Ruby R, Stefanovski D, Johnson AL.To measure and compare CSF cytokine concentrations among horses with equine neuroaxonal dystrophy (eNAD)/equine degenerative myeloencephalopathy (EDM), horses with cervical vertebral stenotic myelopathy (CVSM), and control horses and to evaluate for associations with clinical parameters. Unassigned: Banked equine CSF samples from horses with neurologic disease that underwent a complete neurologic examination and postmortem diagnosis confirmation of CVSM or eNAD/EDM or from control horses were included. Cytokines were measured with an equine-specific cytokine/chemokine magnetic bead multiplex p...
Independent Development of Resistance to Main Classes of Anthelmintics by Gastrointestinal Nematodes of Ruminants and Horses.
Pathogens (Basel, Switzerland)    September 5, 2025   Volume 14, Issue 9 898 doi: 10.3390/pathogens14090898
Cabaret J.Resistance to anthelmintics in gastrointestinal nematodes (GINs) is highly prevalent, as these parasites have been treated with anthelmintics for decades in ruminants and horses. Anthelmintics belong to different classes, each with a different mode of action. The most used are benzimidazoles and macrocyclic lactones and, to a lesser extent, levamisole and pyrantel in herbivores, as estimated from the literature. Combining these classes should be effective in controlling GIN. However, several farmers' practices tend to promote GIN resistance. Therefore, it is unclear whether the use of anthelmi...
Comparison of Two Surgical Techniques for the Treatment of Equine Hindlimb Proximal Suspensory Desmopathy.
Animals : an open access journal from MDPI    September 4, 2025   Volume 15, Issue 17 2598 doi: 10.3390/ani15172598
Freeman KD, Adams MN, Salinger AE, White NA, Barrett JG.Suspensory desmopathy is a frequently documented cause of lameness in performance horses; proximal suspensory desmopathy is the most frequent site of injury in dressage horses [...].
Cushing’s Disease in the Animal Kingdom: Translational Insights for Human Medicine.
International journal of molecular sciences    September 4, 2025   Volume 26, Issue 17 8626 doi: 10.3390/ijms26178626
Massardi E, Gaudenzi G, Carra S, Oldani M, Rybinska I, Persani L, Vitale G.Cushing's disease (CD) is a rare neuroendocrine disorder caused by ACTH-secreting pituitary adenomas, presenting significant diagnostic and therapeutic challenges. Given the evolutionary conservation of the hypothalamic-pituitary-adrenal axis, this review explores the translational value of spontaneous CD forms in dogs, horses, cats, small mammals, and rats, as well as of experimental models in mice, rats, and zebrafish. Dogs are the most studied, showing strong molecular and clinical similarities with human CD, making them valuable for preclinical drug and diagnostic research. While equine an...
Traumatic brain abscess due to Streptococcus equi subspecies zooepidemicus in a Foal.
Veterinaria italiana    September 4, 2025   Volume 61, Issue 3 doi: 10.12834/VetIt.3725.34811.2
Poci Palumbo MI, Maciel Cavalcante R, Martins Amorim R, De Vasconcelos Machado VM, Sousa Rocha N, Garcia Ribeiro M, Cagnini DQ, Secorun Borges A.Bacterial infection of the equine central nervous system is rare. This report describes the clinical features, computed tomography (CT) findings, and postmortem results of a 3-month-old female Quarter Horse with an intracranial abscess. Clinical signs included seizures, depression, and bilateral blindness. CT imaging demonstrated a large space-occupying lesion in the left cerebral hemisphere, associated with a frontal bone fracture. Necropsy and histopathology confirmed the presence of an abscess secondary to head trauma. Bacterial culture identified Streptococcus equi subsp. zooepidemicus as...
Equine Sarcoid Arising From the Medial Caruncle With Invasion of the Third Eyelid in a Quarter Horse Gelding.
Veterinary ophthalmology    September 4, 2025   Volume 29, Issue 2 e70074 doi: 10.1111/vop.70074
Plotsker NM, Taylor R, Coffin HR, Beatty A, Newbold G, Knickelbein KE.To describe the clinical and histologic features of a sarcoid arising from the medial caruncle with spread to the third eyelid of a horse. Methods: A 20-year-old Quarter Horse gelding. Methods: An incisional biopsy of the medial caruncle mass was initially performed, with histopathology suggesting a sarcoid or other spindle cell neoplasm. The mass grew rapidly following the biopsy, expanding to affect the third eyelid and adjacent superior palpebral conjunctiva. A complete third eyelid and medial caruncle excision with resection of affected palpebral conjunctiva and adjunctive cryotherapy was ...
A case-matched controlled retrospective performance indexed analysis of Thoroughbred racehorses that underwent surgical repair of proximal phalanx and third meta-carpal/tarsal condylar fractures.
Equine veterinary journal    September 4, 2025   doi: 10.1111/evj.70088
Yeomans A, Martinkovich D, O'Brien T, O'Neill H, Carpenter R, Georgetti M, Cramp P.Standing fracture repair has become established as an acceptable technique with a good long-term prognosis. However, no study has compared racing outcomes with case-matched controls. Objective: To compare racing outcomes between a large dataset of horses undergoing standing fracture repair against case-matched controls. Methods: Retrospective case-matched controlled comparative study. Methods: Thoroughbred racehorses undergoing standing repair of distal limb fracture(s) over 10 years (2012-2022) at five hospital centres across North America and Europe were included. Data retrieved included...
Progress in serology and molecular biology of equine parasite diagnosis: sustainable control strategies.
Frontiers in veterinary science    September 4, 2025   Volume 12 1663577 doi: 10.3389/fvets.2025.1663577
Wang T, Chen X, Yan X, Su Y, Gao W, Liu C, Wang W.Internal parasitic infections are a persistent challenge for horse owners, in the absence of effective vaccines and the growing challenge of drug resistance, leading many researchers to view current control strategies as unsustainable. Despite slow progress over the past two decades, effective parasitic diagnosis remains crucial for controlling infections and preventing the growing issue of drug resistance. This review examines the research progress in serological and molecular biological diagnostic methods for major equine parasites. Currently, most diagnostic techniques are based on genes su...
A cohort study of nutritional secondary hyperparathyroidism in horses in Hawai’i.
Research in veterinary science    September 4, 2025   Volume 196 105868 doi: 10.1016/j.rvsc.2025.105868
Hunyadi LM, Sundman EA.Equine nutritional secondary hyperparathyroidism (NSHP, Bighead Disease) is a consequence of diets with abnormal calcium: phosphorus ratios. With the widespread availability of fortified feeds and legume forage, the disease has largely disappeared. The Hawaiian Islands are unique in that legume forage is largely unavailable and pastures have high oxalate concentrations. This was a cohort study that included seventeen adult horses imported from the continental US to the Waiki'i region, Hawai'i that grazed on kikuyu grass pastures. Plasma ionized calcium and parathyroid hormone concentrations at...
Effects of sand, asphalt, and a three degrees hind toe or heel elevation on equine cervical and thoracolumbosacral kinematics at walk and trot.
Journal of equine veterinary science    September 4, 2025   Volume 154 105697 doi: 10.1016/j.jevs.2025.105697
Caure S, Miljak K, Dendaw P, Thesée L, Villedey E, Malinvaud A, Cousty M, Prié V, Horan K, Weller R.Effects of ground surface and hoof angles on equine cervical and thoracolumbosacral kinematics are poorly understood. However, the equine cervical and thoracolumbosacral areas present frequent lesions and he management of factors that might improve treatment and rehabilitation outcomes, such as ground surface and hoof angles, requires more investigation. Objective: Our objectives were to determine the influence of ground surface (asphalt versus sand) and a 3 degrees hind toe or heel elevation on cervical and thoracolumbosacral kinematics during walking and trotting. Methods: Six saddle horses ...
Mitohormesis and Regeneration: Natural Compounds Chlorogenic Acid (CGA) and Isovanillic Acid 3-O-sulfate (IVAS) Boost Muscle Cell Recovery in the Equine Athlete Model.
Stem cell reviews and reports    September 3, 2025   Volume 21, Issue 8 2654-2666 doi: 10.1007/s12015-025-10959-9
Witkowska-PiÅ‚aszewicz O, Nowicka-Kazmierczak M, Pietrzak P, Marycz K.Skeletal muscle satellite cells ( SCs), essential for muscle regeneration, are a valuable model for studying exercise-induced stress relevant to human athletes. This study examined the effects of two natural compounds-chlorogenic acid (CGA) and isovanillic acid 3-O-sulfate (IVAS)-increasingly recognized as components of modern, nature-based recovery strategies. Their combination (Hybrid) was also tested on equine model of skeletal muscle satellite cells (ESCs) exposed to heat shock (40 °C, 1 h), mimicking exercise stress. Cells were treated with CGA (0.005%), IVAS (0.0005%), or both for 24Â...
Seropositivity for Pathogenic Leptospira in Dogs, Cats, and Horses at a Teaching Veterinary Hospital in Southern Chile.
Tropical medicine and infectious disease    September 3, 2025   Volume 10, Issue 9 253 doi: 10.3390/tropicalmed10090253
Azócar-Aedo L, Meniconi G, Pino-Olguín C, Gallardo M.At a veterinary hospital in southern Chile, we conducted an epidemiological study involving domestic dogs, cats, and horses to determine the seropositivity for pathogenic spp., identify the infecting serogroups, measure antibody titers, and characterize seropositive animals by sex and age. None of the sampled animals showed clinical signs of leptospirosis. The microscopic agglutination test, using a panel of eight serogroups, was used for diagnosis. The seropositivity was 36.5% (95% confidence interval [CI] = 24.5-48.4) in dogs, 12.9% (95% CI = 2.6-23.1) in cats, and 45.2% (95% CI = 30.1-60.2...