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Topic:Equine Research
Equine biomechanics involves the study of the mechanical principles that govern the movement and structure of horses. This field examines the anatomical and physiological aspects that contribute to locomotion, including gait patterns, joint function, and muscle dynamics. Researchers utilize advanced technologies such as motion capture and force plate analysis to assess the efficiency and performance of equine movement. Understanding biomechanics aids in optimizing training regimens, improving performance, and preventing injuries. This page compiles peer-reviewed research studies and scholarly articles that explore the mechanics of equine movement, its applications in sports and rehabilitation, and its impact on overall equine health.
Detection of ionisable lipids in equine plasma by supported liquid extraction and liquid chromatography-high-resolution tandem mass spectrometry for doping control of mRNA agents. Messenger ribonucleic acid (mRNA) has emerged as a new class of therapeutic agent with unprecedented potential. In particular, the lipid nanoparticle (LNP)-mRNA formulations enabled safe and effective delivery of mRNA in vivo, leading to the success of mRNA vaccines during the COVID-19 pandemic. To control the potential misuse of LNP-mRNA agents in equine sports, a simple and sensitive method has been developed for the detection of ionisable lipids in equine plasma using supported liquid extraction (SLE) followed by liquid chromatography-high-resolution tandem mass spectrometry. To overcome th...
Factors Influencing Equine Embryo Outcomes in OPU‑ICSI Systems: Insights from a Large‑Scale Program in Brazil. Equine OPU‑ICSI supports commercial IVEP, yet substantial variability persists. We retrospectively analyzed a large dataset generated in Brazil spanning Quarter Horses, Warmbloods, and Mangalarga to evaluate whether predictors previously identified in the literature show comparable patterns under distinct ecological and breeding conditions. Overall oocyte and embryo outcomes were aligned with broadly reported trends from previous studies. Oocytes retrieved from estrous mares bearing a dominant follicle exhibited higher maturation and blastocyst rates. Stallion effects segregated into distinc...
Prevalence and radiographic features of cyst-like lesions in German Warmblood horses presented for pre-purchase examination. Cyst-like lesions (CLLs) are frequently detected during radiographic examinations of equine limbs. They may represent a potential risk factor during pre-purchase examination (PPE) and are of considerable clinical and economic importance, as they may lead to negative purchase decisions. While affected horses may develop lameness, incidental findings have also been reported. The goal of the study was to describe the prevalence, location and radiographic characteristics of CLLs in a group of sound German Warmblood horses. Unassigned: A retrospective cohort study was conducted on 3863 German Warmb...
USE OF GUAIFENESIN-KETAMINE-XYLAZINE INFUSION FOR MAINTENANCE OF ANESTHESIA IN CAPTIVE PREZEWALSKI HORSES (EQUUS FERUS PRZEWALSKI ): A CASE SERIES. This case series documents the use of triple drip, a combination of IV guaifenesin, ketamine, and xylazine (GKX) to extend the length of anesthesia in zoo-owned Przewalski horses (). In three separate procedures involving two different animals, GKX was used to prolong and deepen the plane of anesthesia for 90 to 120 min beyond an initial induction dart containing ketamine, butorphanol, azaperone, and medetomidine. The depth of anesthesia was sufficient to permit surgical excision of SC abscesses and other procedures. Throughout the anesthetic events, the horses breathed spontaneously and maint...
Rapid bacterial community profiling of equine faecal, skin, milk and saliva samples using Oxford Nanopore long-read 16S rRNA amplicon sequencing. The composition of the equine gut microbiome is associated with many aspects of gastrointestinal, respiratory and musculoskeletal health that have been reported in the horse. Scientific studies exploring the microbiome non-intestinal ecological niches in or on horses are lacking. The clinical use of bacterial community profiling in horses is currently limited by cost and by slow analytical workflows. Most equine microbiome studies have relied on 16S rRNA amplicon sequencing of bacterial DNA, using high-throughput short-read sequencing technologies. This is often provided by an external servic...
Sixty minutes of daily solitary turnout improved physiological and behavioral indicators of stress and welfare in stall-housed horses. Housing horses in stalls restricts natural roaming behaviors, which in turn increases stress and stereotypic behaviors. Daily turnout for voluntary exercise is common for stress mitigation, but there is no consensus for optimal duration of turnout sessions. Objective: We sought to understand how minimal and moderate-duration daily turnout affects behavioral and physiological stress indicators. Methods: In a Latin-square design, adult Quarter horses (N=11) were allowed 0, 15, or 60 minutes/day of solitary turnout for 12-day periods. In-stall behaviors and 24-hour time budgets were assessed on d...
Repeatability and test-retest reliability of thermal and pressure pain threshold testing in healthy thoroughbred horses. Quantitative sensory testing (QST) has been used in horses to assess nociceptive responsiveness but its broader clinical application is limited by variability across anatomical sites, environmental conditions, operator technique, and behavioral endpoints. This study aimed to evaluate the feasibility, within-session repeatability, and test-retest reliability of thermal threshold (TT) and pressure pain threshold (PPT) testing in healthy Thoroughbred horses. Unassigned: Eleven adult Thoroughbred horses (4 mares, 7 geldings; median [IQR] age 6 [5-6] years; body weight 558 ± 47 kg) underwent...
Effects of flow-controlled expiration on oxygenation and early recovery in horses undergoing general anaesthesia. FLow-controlled EXpiration (FLEX) has been shown to significantly enhance oxygenation in horses under clinical and experimental conditions. Objective: To compare pulmonary gas exchange during early recovery following general anaesthesia in horses ventilated with FLEX compared with conventional intermittent positive pressure ventilation (IPPV) and to assess recovery characteristics between ventilation modes. Methods: Randomised, crossover in vivo experiments. Methods: Six adult horses underwent two 2-h anaesthetic episodes in a randomised crossover design (FLEX vs. IPPV), separated by a two-wee...
Spatiotemporal dynamics of equine group formation reveal the gradual emergence of social cohesion. Stable social relationships are essential for horse welfare, yet domestic management often involves regrouping unfamiliar individuals, potentially causing social stress. Fine-scale dynamics of social integration remain poorly quantified due to limitations of observational sampling. Using ultra-wideband real-time positioning, we tracked the development of spatial and social relationships in two newly formed equine groups (n = 19) over 2-12 months. Inter-individual distances decreased significantly, with time spent within 3 m of conspecifics increasing from 1.2 to 27.3% within two months, d...
Correction: Phenylbutazone concentrations in synovial fluid following administration via intravenous regional limb perfusion in the forelimbs of six adult horses. [This corrects the article DOI: 10.3389/fmed.2026.1810077.].
Vietnam’s Horse Sector: A Comprehensive Review of History, Production Systems, Health Challenges, and Research Priorities. Horses in Vietnam have a long historical presence and continue to hold economic, socio-cultural, and medicinal importance across diverse regions. Despite these roles, comprehensive scientific information on equine development, including health and disease patterns, reproductive performance, genetic resources, and biomedical applications, remains limited and fragmented. In this review, we summarize existing knowledge on equine populations, production systems, and their development in Vietnam, as well as their roles in cultural practices, livelihoods, and traditional medicine. We then identify m...
A point-based laminitis risk scoring system and machine learning framework for prediction of laminitis in horses. Laminitis is a painful and potentially life-threatening inflammatory condition of the equine hoof, and its detection remains a major challenge in veterinary practice. Although several risk factors have been reported, the prediction of the onset of laminitis in a subclinical stage using routine clinical data is still limited. This study aims to address this gap by developing an interpretable machine learning (ML) framework for the prediction of the risk of laminitis in horses. A point-based risk scoring system was first constructed using multivariate logistic regression to quantify the contribu...
Inertial measurement units are comparable to motion capture for measuring intersegmental spinal angular velocities in horses. To compare inertial measurement unit (IMU)-derived intersegmental spinal motion data with measurements obtained from a marker-based motion-capture system. Unassigned: 4 horses were instrumented with custom marker clusters incorporating a 3-D-printed base designed to secure the IMU, ensuring both systems recorded data from the same anatomical location and orientation. A total of 3,737 paired time points across 37 gait cycles were analyzed. Agreement in intersegmental angular velocities across 3 planes of motion was evaluated using Bland-Altman analysis and intraclass correlation coefficients. U...
Diagnostic criteria for equine thoracolumbar myofascial pain syndrome: A foundational study. Diagnostic criteria for myofascial pain syndrome (MPS) are well established in humans but remain underexplored in horses. This study aimed to identify which MPS clinical signs were present in the equine thoracolumbar region, assess internal coherence among clinical indicators, and explore the diagnostic relevance of owner-reported behavioural, signalment, management, and clinical variables. A population of 120 horses underwent a static physical assessment of the thoracolumbar region. Owner-reported horse behaviours were collected through a questionnaire. Taut band, hypersensitive spot, local t...
Physiological Evaluation of Quarter Horses Undergoing Five-Month Taming Focused on Hippotherapy. Preparing horses for hippotherapy programs requires management approaches that prioritize animal welfare and facilitate adaptation to novel stimuli. This study monitored physiological responses during the implementation of a progressive taming protocol for Quarter Horses intended for hippotherapy. Seven animals were monitored over a five-month period, including an acclimation phase and three taming stages. Continuous heart rate, plasma cortisol concentration, and ocular infrared thermography were assessed before and after exercise. Baseline ( = 0.0466) and minimum ( = 0.0095) heart rate values...
Dendritic-cell diversity in equine blood revealed by single-cell transcriptomics. Detailed classification of equine dendritic cells (DC) is necessary to address various research questions, such as the role of DC subsets in immune-mediated diseases of horses. Unassigned: We applied single-cell RNA sequencing (scRNA-seq) on DC enriched from the blood of two horses. Unassigned: All main DC subsets were detected by key gene expression, including conventional DC type 1 (cDC1; ) and type 2 (cDC2; , ), as well as plasmacytoid DC (pDC; ). In addition, we detected a small cluster of hematopoietic progenitors, as well as transitional DC (tDC; , ) and putative DC type 3 (DC3; , ). Our...
Validation of a smart textile electrocardiographic monitoring device in racehorses during high-speed exercise. Exercise-associated cardiac arrhythmias may contribute to poor performance and sudden cardiac death in horses. Widespread monitoring is limited by device availability and suitability for field use. Objective: To (1) compare electrocardiographic (ECG) quality from a smart textile system (Myant Skiin Equine) with a reference device (Televet II) during exercise, and (2) evaluate whether arrhythmia detection with Myant Skiin Equine is comparable to the Televet II. Methods: Prospective blinded clinical study. Methods: Fifty racehorses (25 Thoroughbreds, 25 Standardbreds) underwent up to three high-...
Smartphone-Based Technologies in Equine Sports Medicine: Supporting Athlete Management-A Review. Equine sports medicine is increasingly oriented toward objective, field-based monitoring systems that support both performance optimization and welfare assessment. In this context, smartphone-based technologies have emerged as accessible tools capable of integrating data acquisition, processing, and interpretation within a single platform. This narrative review aims to examine the role of smartphones in equine sports medicine, focusing on their function as standalone sensing devices and as gateways for wearable and external sensor systems. The analysis is based on a structured synthesis of cur...
Detecting and measuring respiratory events in horses during exercise with a microphone: deep learning vs. standard signal processing. Monitoring respiration parameters such as respiratory rate could be beneficial to understand the impact of training on equine health and performance and ultimately improve equine welfare. In this work, we compare deep learning-based methods to an adapted signal processing method to automatically detect cyclic respiratory events and extract the dynamic respiratory rate from microphone recordings during high-intensity exercise in Standardbred trotters. Results: Our deep learning models are able to detect exhalation sounds (median F1 score of 0.94) in noisy microphone signals and show promising r...
Matched embryo-endometrium RNA-seq reveals coordinated but asymmetric transcriptomic reprogramming at the onset of early equine pregnancy. Maternal recognition of pregnancy (MRP) in the mare requires coordinated adaptations of both the conceptus and the endometrium during the peri-recognition interval; however, integrated transcriptomic analyses of both compartments within the same biological window remain limited. Here, we analyzed conceptus RNA-seq data across embryonic age (Days 8 and 12 post ovulation) and endometrial RNA-seq data across pregnancy status (pregnant versus non-pregnant mares sampled between Days 8 and 12 post ovulation). In pregnant mares, conceptus recovery and endometrial biopsy collection were performed with...
Polygenic Prediction of Equestrian Sport Discipline Among Horses Bred for Jumping and Dressage. Horses bred for different equestrian sports vary in physical, physiological and behavioural requirements. Characterising genetic markers associated with discipline provides an opportunity to improve identification of horses best suited to either jumping or dressage. Using a composite selection signals test, genomic regions under positive selection in German warmblood horses divergent for discipline were identified that contained lead SNPs proximal to or within genes with functions relevant to the requirements for jumping and dressage. Dressage genes function in tortuosity of movement (GRM5) an...
Forage nutritive analysis in practical equine feeding: challenges and opportunities. Previous research has shown that forage nutritive analyses are not used to any large extent in equine nutrition. As forages differ widely in nutritional quality, knowledge of the nutritive value is essential to avoid under- or overfeeding of horses. Objective: The aim of this study was to investigate how forage nutritive analyses were used among horse owners in Sweden, and to identify challenges horse owners encounter with such analyses. Methods: Data was collected through an online survey with questions about the respondent, access to forage analytical reports, if and how the respondent used ...
Gross and Histopathologic Comparison of the Distal Third Metacarpal Bone and the Proximal First Phalanx with Sodium Fluoride Positron Emission Tomography Radiopharmaceutical Uptake in Five Horses. Injuries of the metacarpophalangeal joint are a major cause of morbidity and catastrophic fracture in racing horses, yet early osseous pathology is often difficult to detect using conventional imaging. This pilot study aimed to correlate sodium fluoride Positron Emission Tomography (F-NaF PET) radiopharmaceutical uptake with gross and histopathologic changes in the distal third metacarpal bone (MC3) and proximal first phalanx (P1). Five horses (three racing Thoroughbreds with fetlock injury and two non-racing controls) underwent ante-mortem F-NaF PET and cone-beam CT imaging (CBCT), followed b...
Differences in Stride Characteristics Between Lead and Wheel Horses in Competitive Chuckwagon Racing. In Chuckwagon racing, teams of four Thoroughbreds with two lead horses in front and two wheel horses directly behind pull a 600 kg wagon around a racetrack. Our study focuses on stride parameters that have previously retrospectively predicted impending injuries. We hypothesize that in aid of the initiation of the tight figure-of-eight turn at the start of the race, the more agile lead horses gallop with higher stride frequency (SF) and reduced stride length (SL), and that differences are exacerbated in the initial accelerative race segments. Methods: Speed, SF, and SL were quantified with glob...
Transcriptomic and Meat Quality Differences in Longissimus Dorsi Muscle of Surgically Castrated Three-Year-Old Kazakh Horses. Although the Kazakh horse is a dual-purpose breed renowned for both milk and meat production, the extent to which surgical castration alters gene expression in its muscles has not yet been fully elucidated. In this study, left longissimus dorsi muscle (LDM) samples were obtained from six Kazakh stallions (W group) and six Kazakh geldings (S group) to comparatively evaluate meat quality parameters, examine histological characteristics in tissue sections, and apply transcriptomic profiling to comprehensively explore the principal regulatory pathways and candidate genes through which surgical cas...
Phenotypic characterization through identification of gait and coat color phenotype of the Mountain Pleasure Horse. Phenotypic characterization of traits like gait and coat color is useful for minor breed identification and conservation. The Mountain Pleasure Horse (MPH) is a minor American breed as it is listed as threatened by the Livestock Conservancy. Objective: The study objective was to utilize phenotypic characterization for identifying gait and coat color phenotype of the MPH. Methods: Gait was characterized utilizing frame-by-frame analysis to identify spatiotemporal variables of purebred horses (n = 6) performing the flat walk and saddle rack. Coat color was characterized using coat color assi...
Mastication characterization of horses fed bermudagrass hay in a two-part field study. Bermudagrass (Cynodon dactylon L.) is a common forage in the southeastern United States but remains controversial in equine diets due to reported associations with impaction colic. Reduced fiber digestibility has been proposed as a contributing factor, however differences in mastication of fine-stemmed bermudagrass hay have not been studied and may play a role in digestive disturbances. Objective: To compare chewing characteristics, including chews per unit weight and chews per minute, in horses consuming bermudagrass hay compared to other common hay types. Methods: Two studies were conducted....
