Analyze Diet

Topic:Feeding Practices

Feeding practices in horses encompass the methods and strategies involved in providing nutrition to equine animals. These practices are designed to meet the dietary requirements necessary for maintaining health, supporting growth, and optimizing performance. Key components of equine feeding practices include the selection of forage and concentrates, balancing nutrients such as carbohydrates, proteins, fats, vitamins, and minerals, and considering factors such as age, workload, and health status. Feeding practices also address the timing and frequency of meals to align with the horse's digestive physiology. This page compiles peer-reviewed research studies and scholarly articles that explore various aspects of feeding practices, including nutritional requirements, feeding behavior, and the impact of diet on equine health and performance.
Safety and efficacy of feed additives consisting of tinctures derived from the roots or leaves and stems of Althaea officinalis L.: Marsh mallow root tincture for use in all animal species and marsh mallow leaf tincture for use in horses and dogs (FEFANA asbl).
EFSA journal. European Food Safety Authority    October 9, 2026   Volume 24, Issue 10 e10346 doi: 10.2903/j.efsa.2026.10346
Villa RE, Azimonti G, Bonos E, Christensen H, Durjava M, Dusemund B, Gehring R, Glandorf B, Kouba M, López-Alonso M, Marcon F, Martelli G, Nebbia C....Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of two tinctures obtained from the roots (marsh mallow root tincture) or leaves and stems (marsh mallow leaf tincture) of L. when used as sensory additives. The additives are water/ethanol tinctures, with a specified dry matter content of 2%-3% (w/w) (marsh mallow root tincture) and not more than 25% (w/w) (marsh mallow leaf tincture). Marsh mallow root tincture is intended for use in feed or water for drinking for all animal species, while marsh mallow leaf tincture is ...
Characterizing dietary and exercise management practices and inflammatory biomarkers in a population of recreationally managed horses in Pennsylvania.
Journal of equine veterinary science    October 8, 2026   106172 doi: 10.1016/j.jevs.2026.106172
Hall SM, Friend MM, Smarsh DN, Staniar WB.Horses evolved as slow-moving grazers for 30 to 40 million years, but domesticated horses often consume greater quantities of grains and concentrates to foster athleticism. The changes in diet and exercise are hypothesized to increase the risk of chronic inflammatory conditions. Objective: The objective was to characterize relationships between dietary and exercise management practices and acute phase proteins (APPs) in horses. Methods: We collected body condition scores, blood samples, and surveys of the horse management practices from 144 horses. Plasma serum amyloid A (SAA), C-reactive prot...
The use and perception of hay nets and slow feeders by horse owners and handlers: findings from a United Kingdom survey.
Journal of equine veterinary science    October 7, 2026   106171 doi: 10.1016/j.jevs.2026.106171
Amaje J, Upton S, Garba UM, Jolayemi KO.Hay nets and slow feeders are widely used in equine management, yet United Kingdom (UK) horse owners' perceptions of these devices are underexplored. An online cross-sectional survey (66 questions) was distributed via social media between 5 July and 6 August 2024, yielding 100 valid responses from UK horse owners. Data were analysed descriptively and using chi-square/Fisher's exact tests. Hay nets only were used by 42 respondents, slow feeders only by 26, and both by 32. Safety concerns were reported by 43% of hay net users, most often entanglement and dental issues, although 81% used hay nets...
Forage chemical composition drives early metabolic shifts in ponies fed different haylages.
Equine veterinary journal    October 6, 2026   doi: 10.1002/evj.70340
Moore-Colyer MJS, Daniels S, Harris P.Rapid changes between feeds can cause dysbiosis and colic in horses. The metabolic consequences of transitioning between haylages (HY) are currently unknown. Objective: To characterise urinary and faecal metabonomics in ponies transitioned between HY varying in botanical composition and physiological age and to evaluate the speed of metabolic adaptation. Methods: In vivo experiments repeated Latin Square. Methods: Eight Welsh Mountain ponies were fed four HY (R1, R2, M1, M2) across 4 × 15-day periods; a structured 3-day (Days 13-15) changeover was observed: old:new 70:30, 50:50and 30:70....
Evaluating a novel automatic feeding device for hay provision in horses: behavioral outcomes in group and individual housing.
Frontiers in veterinary science    October 6, 2026   Volume 13 1891331 doi: 10.3389/fvets.2026.1891331
Raspa F, Favaro M, Penazzi L, Vasile C, Bordin C, Cavallini D, Ellis AD, Harris P, Valle E.This study evaluated a novel Automatic Feeding Device (AFD) for hay provision in group-housed and individually housed horses. Unassigned: In Phase 1 (P1), behavioral responses to ground hay feeding (Control-Hay) and to hay delivery through the AFD were assessed in three group-housed mares under two conditions: AFD continuously open (O-AFD) and AFD alternating between open and closed periods (OC-AFD). In Phase 2 (P2), four individually housed horses were used to evaluate the behavioral effects of three AFD opening-closing schedules: long opening (LO-OP), medium opening (ME-OP), and short openin...
The effects of feeding frequency on glycemia and insulinemia in moderately exercised young horses.
Research in veterinary science    October 5, 2026   Volume 212 106428 doi: 10.1016/j.rvsc.2026.106428
Hess T, da Costa Santos H, Black J.Concentrates are typically fed twice daily to supplement forages in horses to facilitate management, especially those with higher energy needs, but high-grain meals can contribute to digestive and metabolic challenges, including insulin dysregulation. This study investigated the effects of feeding frequency on glycemia, insulinemia, and insulin sensitivity measured by an oral sugar test in moderately exercised young horses. Thirty-two healthy Quarter Horses (2-3 years old) were assigned to either traditional feeding (TF; two grain meals/day) or fractioned feeding (FF; automated grain delivery...
A novel water additive increases voluntary water intake in healthy horses.
Journal of the American Veterinary Medical Association    October 2, 2026   1-5 doi: 10.2460/javma.26.05.0336
Yancey K, Hassel D, Rao S, Seabaugh KA.To determine whether healthy horses would increase their voluntary water intake (VWI) when offered a novel water additive (NWA) compared to plain water (TAP) and water flavored with sweet feed, referred to as sweet tea (ST). Unassigned: 12 clinically healthy horses, aged 2 to 5 years old, were enrolled in the experimental crossover design. Four horses were assigned to each group for each of the 3 study weeks. The order that the horses were allocated to study groups was randomly assigned. All horses had access to 2 buckets filled to 16 L, one containing plain water (untreated bucket) and one co...
Characterisation of microbial metabolite profiles across the gastrointestinal tract of horses fed on pasture, hay or oat-supplemented rations.
PloS one    September 28, 2026   Volume 21, Issue 9 e0359277 doi: 10.1371/journal.pone.0359277
Santo MM, Bachmann M, Schusser GF, Wensch-Dorendorf M, Pisch C, Bochnia M, Hüther L, Heinichen K, Zeyner A.Dietary composition substantially influences the intensity and site of microbial metabolite production in the gastrointestinal tract. These metabolites have numerous beneficial effects on equine health, but may also be involved in the development of gastrointestinal disorders. It remains unclear whether different feeding strategies, including starch supplementation, can alter microbial metabolite profiles along the gastrointestinal tract. This study aimed to compare the metabolite profiles of the microbial communities in the gastrointestinal tract of horses fed on pasture 24 h/day (PST; n =â...
Beyond Body Condition Score: A Proposed Interspecies Framework for Tissue-by-Tissue Nutritional Assessment in Veterinary Practice.
Veterinary sciences    September 10, 2026   Volume 13, Issue 9 938 doi: 10.3390/vetsci13090938
Cappai MG.In clinical veterinary medicine, the Body Condition Score (BCS) serves as a validated, semi-quantitative estimation of subcutaneous adipose reserves, yet it cannot by itself detect systemic nutrient deficiencies or excesses that do not necessarily imply energy storage. Human clinical dietetics addressed an analogous diagnostic gap through the Nutrition-Focused Physical Examination (NFPE), but veterinary medicine still lacks a unified, multi-species physical examination sequence for nutritional screening. This narrative review synthesizes the comparative and clinical nutrition literature into a...
Relationship Between Feed Olfactory Exploration and Consumption in Horses.
Animals : an open access journal from MDPI    August 20, 2026   Volume 16, Issue 16 2599 doi: 10.3390/ani16162599
Wnuk E, Janicka W, Łuszczyński J, Wojtyniak A, Jagusiak W, Stachurska A.The aim of the study was to evaluate the relationship between horses' behaviour during olfactory exploration and following consumption of the same food containing herbs, either previously exposed to the herb odour or not. Twenty horses' responses to 14 herbs were compared in a smelling test, when the horses sniffed a herb, and after 20 min a consumption test, when they consumed oats mixed with the same herb. Before the tests, the horses were exposed to the scent of seven herbs, whereas the remaining seven herbs were non-pre-exposed. The results show that horses consumed oats with herbal additi...
Transforming retraining: Supporting racehorses to have successful second careers.
PloS one    August 19, 2026   Volume 21, Issue 8 e0355509 doi: 10.1371/journal.pone.0355509
Williams JM, Jordan S, Friend L, Kay E, Edmunds M, Nankervis K, Cheung J, Tabor G.Optimizing long-term welfare outcomes for Thoroughbred racehorses following retirement from racing is a priority across the global horse racing industry. Following their racing career, horses are required to adapt to a markedly different lifestyle, involving substantial physical, behavioural, and mental adjustment. This study consulted experienced retrainers to identify factors that inform successful retraining and transition of former racehorses to promote positive lifelong outcomes beyond racing. An online survey of 100 global stakeholders experienced in retraining former racehorses explor...
Semi-extensive management system drives lower prevalence of equine orodental disorders.
Frontiers in veterinary science    August 18, 2026   Volume 13 1827412 doi: 10.3389/fvets.2026.1827412
Kunz JR, Peres Mendes R, Granella MCS, Hartl B, de Souza AF, Fonteque JH, Kau-Strebinger S.The prevalence of orodental disorders in horses kept under different management systems is lacking. More detailed knowledge is needed to support targeted therapeutic and prophylactic approaches, as these conditions play a crucial role in maintaining equine health and welfare. This study aimed to compare the prevalence of orodental disorders in horses raised, kept, and fed under extensive, semi-extensive, or intensive management systems. Unassigned: Sixty-nine clinically healthy adult crossbred horses with no history of dental treatment were examined, with 23 horses included in each study group...
The effects of diet on the physical characteristics of feces and fiber digestibility in healthy horses.
Journal of animal science    August 13, 2026   skag255 doi: 10.1093/jas/skag255
Starrett A, Sarturi JO, Leatherwood JL, Chaffin MK, Mukhopadhyay S, Pilla R, Arnold CE.Diet is a known risk factor for colic, but few studies have explored how diet affects fecal characteristics which are often altered in horses with colic. This prospective cross-over study evaluated the effects of two common equine diets on fecal characteristics and fiber digestibility in healthy adult horses. We hypothesized that horses offered a complete pelleted diet would exhibit increased fecal dry matter (DM) and decreased fecal particle size compared to those offered a forage-based diet. Twenty horses at Texas Tech University School of Veterinary Medicine served as subjects in the study....
Choice for the horse is paramount: Owners’ experiences in facilitating friends, forage, and freedom in their management practices.
Animal welfare (South Mimms, England)    August 10, 2026   Volume 35 e48 doi: 10.1017/awf.2026.10107
Watson WL, Jessiman LJ, Dwyer C.Owned horses rely on people for their behavioural needs, such as access to conspecifics, constant access to low nutrient density, fibrous forage and freedom of movement; otherwise known as friends, forage and freedom (3Fs). Restricting the 3Fs prevents horses from achieving optimal welfare. We carried out 14 semi-structured interviews in the UK and Ireland to examine horse owners' experiences, attitudes and beliefs around the provision of the 3Fs. Most participants were female, aged from 25 to 65 years, resident in the UK and kept their horse at a livery facility. A reflexive thematic analysis...
Four Haylages, One Metabonome: Defining Diet Driven Metabolic Shifts in Ponies.
Journal of animal physiology and animal nutrition    August 6, 2026   doi: 10.1111/jpn.70099
Moore-Colyer M, Harris P, Daniels S.Haylage is widely fed to all categories of stabled horses across Europe and New Zealand. Haylage can vary from high energy nutrient dense forage to low energy highly fibrous fodder, yet little is known about the impact different haylages have on metabolism in horses. This study provides the first direct comparison of feeding four different haylages: early cut (Lolium multiflorum), later cut (Lolium perenne) rye grass (R1 and R2); early and late cut meadow grass (M1 and M2), on urinary and faecal metabonomics, in ponies at maintenance. Using replicated Latin Square design, eight individually ho...
Unveiling wolf dietary specialization on free ranging horses using forensic genetics and field monitoring.
Scientific reports    July 20, 2026   doi: 10.1038/s41598-026-59871-3
Freitas J, Godinho R, Mendonça R, Nakamura M, Rio-Maior H, Ringhofer M, Yamamoto S, Hirata S, Álvares F.Wolves occasionally specialize on free-ranging horses when they are abundant and alternative prey is scarce, potentially reducing horse productivity, triggering conflicts with owners, and hampering conservation of both species. Yet, ecological and demographic impacts of wolf predation on horses remain poorly understood. We investigated wolf-horse interactions in NW Portugal by combining wolf monitoring, diet analysis, spatial overlap between wolf and horse activity areas, and horse pedigree reconstruction from genetically identified individuals recovered from wolf scat remains, feeding sites a...
Differential shaping of equine gut microbiota structure and function by breed and feeding regimen.
Frontiers in microbiology    July 16, 2026   Volume 17 1852554 doi: 10.3389/fmicb.2026.1852554
Lin Y, Qiri G, Du M, Dugarjaviin M, Cao J, Fang X, Yun S, Li X, Gong W, Ding W, Bou T, Su S, Chen J, Xing N, Bai D, Zhao Y.The gut microbiota plays an essential role in host energy metabolism and immune function. Horses are non-ruminant herbivores that rely heavily on hindgut microbial fermentation to meet their energy requirements. However, the relative contributions of host genetic background (breed) and environmental factors (feeding regimen and geographical location) to shaping the equine gut microbiota remain poorly understood. Unassigned: In this study, 16S rRNA gene sequencing and functional prediction analysis were performed on 139 equine fecal samples to systematically investigate the differential effects...
Determining optimal practices for foal weaning – A protocol for a systematic review and network meta-analysis.
PloS one    July 1, 2026   Volume 21, Issue 7 e0352182 doi: 10.1371/journal.pone.0352182
Cranston N, Buckley P, Rose L, Loy J, Randle H.Weaning is a stressful time in a foal's (Equus caballus) life. As humans artificially manage weaning in domestic horses, it provides an opportunity to safeguard horse welfare early in life, with potential long-term benefits. While numerous studies have examined the effects of different weaning and management interventions on foal stress, optimal weaning practices have yet to be identified. Overall purpose of the study: To identify which weaning interventions and management practices have the greatest potential to improve foal welfare based on physiological and behavioural outcomes. Research qu...
Short-term provision of moderate dietary starch alters fecal microbiota but does not exacerbate exercise-induced inflammation in yearling Quarter Horses.
Journal of equine veterinary science    June 27, 2026   106071 doi: 10.1016/j.jevs.2026.106071
DiSilvestro AN, Wesolowski LT, Williams BD, Warren LK, Athrey G, White-Springer SH.Energy-dense feeds commonly provided to equine athletes may be high in starch, which alter gastrointestinal microbiota and could promote systemic inflammation. Objective: To test the hypothesis that exercise-induced inflammation would be greater in horses receiving a starch- versus fiber-based concentrate. Methods: Quarter Horses (mean±SD 16±1mo; 337±30kg) received either a fiber-based control (CON; 7 fillies, 8 geldings) or an isocaloric, isonitrogenous starch concentrate (STARCH; 8 fillies, 7 geldings) for 24d. Fecal metagenomics were evaluated on d0 and 21. Blood inflammatory mediators w...
Fecal microbiota of weaned equine and mule foals grazing bermudagrass pastures.
Journal of equine veterinary science    June 27, 2026   106070 doi: 10.1016/j.jevs.2026.106070
Araújo LSA, Franzan BC, Almeida MIV, Coelho IS, Almeida FQ.Weaning is a critical period in the lives of foals. After weaning, forage consumption represents a major challenge for both equine or mule foals. Objective: This study evaluated the fecal bacterial concentration, composition, diversity and differential abundance in weaned equine and mule foals under rotational grazing on Bermudagrass (Cynodon spp.) pasture. Methods: A randomized design was used with equine and mule foals grazing two rotations on three Bermudagrass cultivars (Coastcross, Florakirk, and Tifton-85). Pasture rotation occurred every 20 days. The first rotation was evaluated at 20, ...
In silico genome mining and characterization of putative horse feces-derived bacterial phytases as potential monogastric animal feed additive candidates.
Journal, genetic engineering & biotechnology    June 23, 2026   Volume 24, Issue 3 100731 doi: 10.1016/j.jgeb.2026.100731
Urgessa OE, Gemeda MT, Dinka H, Abdi FG, Tafess K.Phytic acid exerts a significant antinutritional effect in poultry, swine, and fish, which can be mitigated by supplementing monogastric feeds with efficient microbial phytases. Accordingly, mining bacterial genomes for novel phytases represents a strategic computational approach to identifying candidates for improving monogastric animal nutrition. In this study, 162 bacterial genomes associated with horse feces were systematically mined using an in silico pipeline to identify and characterize putative phytases.A total of 69 non-redundant sequences were identified and classified as histidine a...
Forage nutritive analysis in practical equine feeding: challenges and opportunities.
Journal of equine veterinary science    June 18, 2026   106067 doi: 10.1016/j.jevs.2026.106067
Nilsson ST, Müller CE, Lindqvist H.Previous research has shown that forage nutritive analyses are not used to any large extent in equine nutrition. As forages differ widely in nutritional quality, knowledge of the nutritive value is essential to avoid under- or overfeeding of horses. Objective: The aim of this study was to investigate how forage nutritive analyses were used among horse owners in Sweden, and to identify challenges horse owners encounter with such analyses. Methods: Data was collected through an online survey with questions about the respondent, access to forage analytical reports, if and how the respondent used ...
Mastication characterization of horses fed bermudagrass hay in a two-part field study.
Journal of equine veterinary science    June 17, 2026   106068 doi: 10.1016/j.jevs.2026.106068
Long JF, Hutton R, Abrams A, Duberstein JA, Duberstein KJ.Bermudagrass (Cynodon dactylon L.) is a common forage in the southeastern United States but remains controversial in equine diets due to reported associations with impaction colic. Reduced fiber digestibility has been proposed as a contributing factor, however differences in mastication of fine-stemmed bermudagrass hay have not been studied and may play a role in digestive disturbances. Objective: To compare chewing characteristics, including chews per unit weight and chews per minute, in horses consuming bermudagrass hay compared to other common hay types. Methods: Two studies were conducted....
Prevalence of gastrointestinal parasites in horses in the district of Abidjan, Ivory Coast.
Scientific reports    June 12, 2026   doi: 10.1038/s41598-026-56528-z
Kante N, Kaba L, N'Gotta KI, Camara AOD, Salako AS, Berete K, Tuo K, N'Docho AF, Gondo É, Teohou MMC, N'Gotta AA, Traore AK, Barro N.Gastrointestinal parasitic infections constitute a major constraint to equine health and productivity, particularly in tropical regions where climatic and environmental conditions favor parasite survival and transmission. In Côte d'Ivoire, epidemiological data regarding gastrointestinal parasites in horses remain limited, especially in urban and peri-urban settings such as the district of Abidjan. This study aimed to determine the prevalence, diversity, and associated risk factors of gastrointestinal parasites affecting horses in the study area. A descriptive and analytical cross-sectional st...
Plasma and urinary exposure of cannabidiol and cannabidiolic acid in horses following consumption of contaminated feed.
Irish veterinary journal    June 4, 2026   doi: 10.1186/s13620-026-00350-6
Ekstrand C, Michanek P, Hernlund E, Gehring R, Salomonsson M.Cannabidiol (CBD) and cannabidiolic acid (CBDA) are non-psychoactive cannabinoids naturally present in hemp-derived products. While these compounds are prohibited during equine competition, horses may be inadvertently exposed through contaminated feed. Data describing systemic and urinary exposure to CBD and CBDA following such low-dose, involuntary intake are limited. This study aimed to describe plasma and urine concentration-time profiles of CBD and CBDA in horses after consumption of experimentally contaminated feed. Results: Twelve Standardbred horses received feed containing low doses (1...
Do Polish Konik Horses Find Soaked Hay to Be Palatable?
Animals : an open access journal from MDPI    May 29, 2026   Volume 16, Issue 11 1663 doi: 10.3390/ani16111663
Tkaczyk E, Gazda IE, Humięcka M, Łuszczyński J, Basińska K, Kaczmarek B, Barłowska J, Tkaczyk P, Janczarek I.Frequently, equine diseases necessitate the moistening or soaking of hay for the horses. It was assumed that this treatment would not reduce the willingness of the Polish Konik horses with low nutritional requirements to consume the hay. Furthermore, it was believed that moistening would increase the palatability of this feed. The aim of this study was to determine time and frequency parameters and the amount of unconsumed dry, moist, or soaked hay. The research material comprised 12 Polish Konik horses consuming either 1 kg of dry hay, 2.1 kg of moist hay, or 3.3 kg of soaked hay on three sub...
Farm Atmosphere: Calm Attention and Mobility Characterise Positive Horse Welfare.
Animals : an open access journal from MDPI    May 20, 2026   Volume 16, Issue 10 1557 doi: 10.3390/ani16101557
Hausberger M, Lerch N, Grandgeorge M.On-farm welfare assessment of equines is a challenge given the large diversity of management practices, especially in terms of housing and activities. In our study, we tested time budget measures as a complementary tool to more conventional welfare indicators (e.g., stereotypic behaviours, ear position while foraging, neck shape). We observed 174 horses living in eight facilities (in their home environment) for which data on management practices and welfare were available. Time budget was assessed using the scan sampling method (1 min scan sampling over 30 min; 33 scans), while welfare assessm...
Long-Read Sequencing for Species-Level Resolution of the Equine Gut Microbiota Reveals the Need for Improved Databases.
Animals : an open access journal from MDPI    May 9, 2026   Volume 16, Issue 10 1459 doi: 10.3390/ani16101459
Leduc L, Boucher L, Mach N, Leclère M, Costa M.Differences in gut microbiota composition related to diet have been reported in horses, but characterization of specific microbial taxa remains limited, particularly at the species level. The objective of this study was to use long-read sequencing of the 16S rRNA gene to provide additional taxonomic insight into the intestinal microbiota in horses. Fecal samples were collected from 12 horses on pasture and from 6 of them after switching to a hay diet. Sequencing yielded low read counts per sample, and the analysis failed to detect statistical differences in alpha- and beta-diversity among diet...
Welfare Assessment in Equine-Assisted Service (EAS) Horses.
Animals : an open access journal from MDPI    May 8, 2026   Volume 16, Issue 10 1434 doi: 10.3390/ani16101434
Carvalho Seabra J, Hess T, Finnestead T, Grandin T.Equine-Assisted Service (EAS) horses can be exposed to stressors that affect their behavior and welfare. Previous studies investigated behavioral and physiological indicators (PI) only during therapy sessions. This study evaluated the frequency of stress and pain behaviors (FSPB) in horses with different riders, while assessing welfare through housing, management, and PI. Ten horses were observed during EAS sessions with three rider groups: able-bodied, clients with significant physical impairment, and clients with no significant physical impairment, totaling twenty-six riders. Behavioral data...
The effects of citrulline on horse milk components and fecal metabolism were analyzed based on LC-MS and 16s amplicon.
BMC veterinary research    May 7, 2026   doi: 10.1186/s12917-026-05534-4
Ma Y, Liu P, Li X, Hu Q, Lin J, Yang F, Yang K.The lactation period in mares is critical, as it significantly influences foal health and subsequent reproductive outcomes. Developing a precise nutritional strategy for lactating mares is a primary research focus. L-citrulline, a bioactivenon-protein amino acid and precursor to arginine, has been shown to enhance mammary blood flow and reduce oxidative stress, yielding promising results in livestock lactation studies. However, research on its effects in Equus species remains limited. There is a notable gap in data regarding its impact on lactation performance blood biochemistry, and its role ...
1 2 3 … 33