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Topic:Veterinary Science

Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
Genetic diversity of Dermacentor nitens (Acari: Ixodidae) collected on horses from western Cuba.
Journal of medical entomology    May 17, 2026   Volume 63, Issue 3 tjag059 doi: 10.1093/jme/tjag059
Roblejo-Arias L, Diaz-Sánchez AA, Marrero-Perera R, Lobo-Rivero E, Corona-González B, Chilton NB.The tropical horse tick, Dermacentor nitens Neumann, 1897, has a wide distribution throughout the Neotropics, and is a vector of protozoan haemoparasites that cause equine piroplasmosis. Despite this, little is known about the genetic diversity of this tick species, particularly in Cuba, where horses are important domestic animals used in agriculture and for transportation. The objective of this study was to assess the magnitude of genetic variation in 115 D. nitens adults (57 females and 58 males) collected from horses in the Cuban province of Mayabeque using sequence data of two mitochondria...
Alkaline-Promoted Hydrothermal Liquefaction of Polo Horse Manure for Biocrude Production.
ACS omega    May 16, 2026   Volume 11, Issue 21 31510-31520 doi: 10.1021/acsomega.6c01555
Namsiri P, Pianthong K, Thanakijkasem P.Horse manure (collected as pure feces) is a high-moisture, nutrient-dense organic waste stream with potential for valorization via hydrothermal liquefaction (HTL). However, catalyst selection is often reported without direct catalyst-class comparisons under identical conditions. In this study, manure collected in Thailand was characterized and converted via noncatalytic and catalytic HTL in a batch reactor at 300 °C for 40 min (feedstock/water = 1:10, w/w; heating rate 10 °C min). Four catalysts (KOH, NaOH, HPO, NH·HO) were evaluated at 10 and 20 wt % (dry feed basis), with biocrude recover...
Genetic Diversity and Population Structure in Seven Lipizzan Populations Based on Microsatellite Genotyping.
Animals : an open access journal from MDPI    May 15, 2026   Volume 16, Issue 10 1516 doi: 10.3390/ani16101516
Rogić B, Moravčíková N, Margeta P, Štrbac L, Zorc M, Kovács M, Vostry L, Kasarda R, Posta J.Lipizzan horses, bred for over four centuries, represent a unique genetic, cultural and historic resource. This study assessed genetic diversity and population structure across seven European Lipizzan populations, with emphasis on the Bosnia and Herzegovina population. A total of 547 Lipizzan horses were genotyped using 12 microsatellite markers. The parameters of genetic variability, admixture level and migration rate were calculated. The mean number of alleles, effective number of alleles and Shannon's information index were 5.78, 3.24 and 1.31, respectively. The average fixation indices wer...
A synergistic dual-endopeptidase platform for high-yield, homogeneous F(ab’)2 production from polyclonal equine immunoglobulins for therapeutic applications.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences    May 12, 2026   Volume 1280 125111 doi: 10.1016/j.jchromb.2026.125111
Shen CK, Liu J, Xu K, Xu P, Li Z, Wang L, Li C, Ding F, Xiao C, Yao X, Jing Y.Therapeutic F(ab') fragments derived from polyclonal equine antisera remain essential emergency biologics for addressing snake envenomation, tetanus, and viral infections. Traditional production methods rely on pepsin digestion, which is limited by non-specific proteolysis, harsh acidic conditions (pH 2.0-4.0), risk of contamination from animal sources, and significant batch variability, often complicating downstream preparative separations. In this study, we introduce a synergistic dual-endopeptidase platform that integrates hinge-specific biocatalysis with affinity chromatography to facilit...
Comparison of equine PBMC isolation using two protocols: Impact on key cellular parameters.
Research in veterinary science    May 10, 2026   Volume 208 106260 doi: 10.1016/j.rvsc.2026.106260
Magalhães VLP, Pinho FA, Souza FN, Santos KR, Santos AS, Hlavac N, Libera AMPD, Massoco CO, Barrouin-Melo SM.There are diverse uses for equine peripheral blood mononuclear cells (PBMC) in research. Studies have shown that PBMC isolation protocols can affect immune parameters of diagnostic and research assays. We aimed to assess the impact of equine blood handling techniques on cell yield, viability, and proliferation under mitogen stimulation. Whole blood (WB) samples were collected from eight healthy adult horses into heparinized tubes for PBMC isolation by comparing method 1 (M1) where freshly collected WB was layered directly over a density gradient medium for centrifugation, followed by PBMC harv...
The anterograde tarsal venography enhances vessel visualization compared to the retrograde approach in horses.
Veterinary journal (London, England : 1997)    May 8, 2026   106697 doi: 10.1016/j.tvjl.2026.106697
Parra-Torres DL, Melo-Neto GB, Garcia HO, Souza APA, Apolonio EVP, Rodrigues CA, Alves ALG, Hussni CA, Watanabe MJ.The objective of this study was to compare the number of vessels of the proximal venous access (ramus cranialis of vena saphena medialis) and the distal venous access (vena digitalis dorsalis communis II) for venography and, consequently, intravenous regional limb perfusion of the tarsal region. Seven horses underwent venographic examination of both hind limbs, divided into two groups: proximal venous access (PG) and distal venous access (DG). Rubber tourniquets were applied to the middle third of the tibia and the proximal third of the III metatarsal bone. Thirty mL of contrast was injected (...
FGF2, LIF, and IGF1 supplementation during in vitro maturation or culture improves equine ICSI embryo development in an age-dependent manner.
Reproduction, fertility, and development    May 7, 2026   Volume 38, Issue 7 RD26023 doi: 10.1071/RD26023
Smith JM, Palacios PD, Segura Forero PA, Gurkin RJ, Doyle T, Boe-Hansen G, Gambini A.Ovum pickup (OPU) combined with intracytoplasmic sperm injection (ICSI) has reshaped equine assisted reproduction, enabling embryo production from mares and stallions with constrained breeding opportunities; however, limited efficiency of in vitro maturation (IVM) and in vitro culture (IVC) continues to hinder embryo production. Growth factor supplementation with fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1) in combination (FLI) has been shown to enhance oocyte and embryo developmental competence in several species. Objective: This...
Genetic diversity of Toxoplasma gondii isolates from members of the families camelidae and equidae worldwide: a systematic review.
Irish veterinary journal    May 7, 2026   doi: 10.1186/s13620-026-00344-4
Hataminejad M, Lashaki EK, Behboudi E, Esmaeili B, Ahmadi S, Galeh TM.Toxoplasma gondii is an apicomplexan parasite with a global distribution and significant public health and veterinary treatment implications. The present systematic review collates worldwide information on the genotypic diversity of T. gondii isolates infecting Camelidae (camels, alpacas, llamas, etc.) and Equidae (horses, donkeys, zebras, mules, etc.) and highlights their potential role as sentinels of environmental contamination and zoonotic spread. An exhaustive literature search of six electronic databases (PubMed, Web of Science, Scopus, ProQuest, Science Direct, and Google Scholar) from ...
Factors influencing gestation length in Mares in the Tiaret region, Algeria.
Journal of equine veterinary science    May 7, 2026   Volume 163 105925 doi: 10.1016/j.jevs.2026.105925
Boubakeur E, Belmdjahed M.Gestation length is a key parameter in equine reproductive management, yet its variability remains poorly documented in Barb and Arabian mares under Algerian conditions. Objective: This study aims to determine gestation length in mares and to assess the influence of various factors, including breed, year of mating, mating month, mare age, parity, reproductive status, stallion and the foal's sex on gestation duration. Methods: A total of 133 mares from the National Stud Farm of Tiaret (Algeria) were monitored over the period 2021-2024. Results: The mean gestation length was 332.5 days (range: 3...
The Equine Reproductive Microbiota: Composition, Dynamics, Dysbiosis, and Implications for Fertility in Mares and Stallions.
Animals : an open access journal from MDPI    May 5, 2026   Volume 16, Issue 9 1414 doi: 10.3390/ani16091414
Pelenė U, Šiukščius A, Nainienė R, Merkelytė I, Šveistienė R.The equine reproductive microbiota has emerged as an important factor influencing reproductive health and fertility in both mares and stallions. Traditionally, the equine uterus was considered sterile, and microbial presence was interpreted primarily in the context of infection. However, sequencing-based studies have demonstrated that the reproductive tract, including the uterus and semen, contains detectable microbial communities or microbial DNA signatures, challenging this traditional paradigm. In mares, the vaginal microbiota is consistently dominated by , , , and , whereas the uterine env...
Quantitative evaluation of behavioral and physiological adaptation during retraining of retired Thoroughbred racehorses.
Journal of equine veterinary science    May 4, 2026   Volume 163 105920 doi: 10.1016/j.jevs.2026.105920
An SJ, Lee JH, Lee HY, Kim BS, Forbes E, Ryu SH.Retired Thoroughbred racehorses commonly transition into riding roles, yet outward behavioral calmness may not fully reflect internal physiological regulation, with implications for safety. Objective: To quantify behavioral and autonomic adaptation across repeated startle exposures and assess concordance between behavioral and physiological indicators. Methods: Twelve retired Thoroughbreds underwent standardized plastic-bag startle trials conducted across three sessions (T1-T3), each including pre-training (Before) and post-training (After) phases, with three repeated exposures at 5-minute int...
The effect of form on equine salt intake.
Journal of equine veterinary science    May 2, 2026   105923 doi: 10.1016/j.jevs.2026.105923
Murphy B, Catalano DN.Sodium and chloride are required nutrients and are typically met using sodium chloride (salt) via compressed blocks. The objective of this study was to compare salt intake using block or loose salt. Sixteen mature horses were blocked by housing style and assigned to block salt or loose salt (form) for four 7-day periods. Salt intake was measured by weight at the end of each period and was not affected by form (P = 0.070) or period (P = 0.902). There was an effect of horse (P < 0.001). Horses consumed between 2 and 135 g of salt daily, however; over half of the horses in the study di...
Historical perspectives guide advances in equine minimally invasive soft tissue surgery.
Journal of the American Veterinary Medical Association    May 1, 2026   Volume 264, Issue 9 1-11 doi: 10.2460/javma.26.01.0067
Rockow M, Gongaware A, Hendrickson D, Easley J, Luedke L, Pezzanite L.Equine minimally invasive soft tissue surgical techniques have increased in demand and frequency with concurrent expansion of training programs under the oversight of the American College of Veterinary Surgeons. Benefits of recent advances in laparoscopic techniques include reduced cost and risk associated with general anesthesia and anesthetic recovery, improved visualization of the surgical field relative to open procedures, and more rapid return to performance, further improving patient welfare and limiting economic loss to the industry following surgical interventions. The evolution of lap...
The immediate effect of whole-body vibration therapy on mechanical nociceptive thresholds in horses with thoracolumbar pain.
Journal of equine veterinary science    April 30, 2026   105919 doi: 10.1016/j.jevs.2026.105919
Giancola SC, Ellis KL.Treating primary back pain in horses can be challenging and often necessitates a multimodal approach. Whole-body vibration therapy (WBV) has been used in both horses and humans to alleviate pain and strengthen muscles. A recent study showed worsening back pain following a 30-minute session of WBV; however, the immediate effects of WBV were not evaluated. The purpose of this study was to assess the immediate effects of whole-body vibration therapy on horses with back pain after a single session of WBV. Objective: The objective of the current study was to determine the immediate effects on mecha...
Nano-Encapsulated Vitamin E Enhances Cryo-Resistance of Stallion Spermatozoa: A Comprehensive Evaluation of Sperm Quality, Kinematic Parameters, Antioxidant Capacity, Microbiota and Ultrastructure.
Reproduction in domestic animals = Zuchthygiene    April 30, 2026   Volume 61, Issue 5 e70213 doi: 10.1111/rda.70213
Khalil WA, Mostafa HE, Derbala MK, Hassan MAE, Abdelnour SA, El-Harairy MA.This experiment explores the comparative ability of vitamin E (VE) and its nano-emulsion (VEN) in enhancing cryo-resistance of stallion spermatozoa by examining semen quality, kinematic variables, acrosome integrity, apoptosis, antioxidant capacity, ultra-structural alterations and microbiota of cryopreserved stallion sperm. Semen from five stallions (8-10 years) was cryopreserved with 0.1 or 1 mM of VE or VEN; and a non-additive group served as the control. The VEN particles ranged in size from 72 to 172 nm, with a mean diameter of 287 nm. Supplementation with VE (1 mM) or VEN (0.1 ...
Horse racing towards antibiotic resistance. Accompanying animals as a source of antibiotic-resistant bacteria.
Journal of applied genetics    April 29, 2026   doi: 10.1007/s13353-026-01053-y
Lepianka A, Sitkiewicz I.Antibiotic resistance is a significant issue in modern infectious medicine and veterinary science, with animals, including horses, playing a crucial role in its dissemination. Antibiotics, used both for treatment and prevention of diseases in animals, influence the composition of the microbiota and promote the selection of resistant strains that can be transmitted to humans through direct or environmental contact. Horses, currently classified as companion animals, serve as a reservoir of commensal and pathogenic bacteria, including multidrug-resistant strains capable of horizontal gene transfe...
CD8+ T-cells, CD86+ macrophages and TNF-α signalling pathways are correlated with fetlock osteoarthritis in racehorses.
Equine veterinary journal    April 29, 2026   doi: 10.1002/evj.70168
Secor EJ, Chow EN, Thomas M, Johnson L, Siefert A, Wagner B, Engiles JB, Reesink HL.There is emerging evidence for the role of the immune system in osteoarthritis (OA) pathophysiology; however, little is known about how immune cells and the synovial transcriptome are altered in naturally occurring equine OA. Objective: To evaluate synovial fluid (SF) and synovial membrane (SM) immune cell populations and the SM transcriptome in racehorses with fetlock OA. Methods: Cross-sectional cadaver study. Methods: Fetlock joints from racehorses euthanised at NY racetracks were considered for inclusion. SF and SM were analysed by flow cytometry using T-cell (CD3, CD4, CD8) and macrophage...
Editorial: Biomarker identification and novel therapies in equine medicine.
Frontiers in veterinary science    April 28, 2026   Volume 13 1843968 doi: 10.3389/fvets.2026.1843968
Żak-Bochenek A, Siwińska N.No abstract available
Comparison Between Inertial Sensor and Video-Based Detection of Spatiotemporal Limb Movement Parameters During Equine Swimming.
Sensors (Basel, Switzerland)    April 28, 2026   Volume 26, Issue 9 2743 doi: 10.3390/s26092743
Marin F, Giraudet C, Gaulmin P, Moiroud C, De Azevedo E, Hatrisse C, Ben Mansour K, Martin P, Audigie F, Chateau H.Equine swimming is increasingly used for injury prevention and rehabilitation, but objective analysis of movement during swimming remains limited compared to land-based locomotion. Spatiotemporal parameters are essential for evaluating therapeutic outcomes, yet capturing these parameters is technically challenging due to difficulties in observing limb motion in water. Inertial sensors, already widely applied in equine science, offer a promising solution for measuring swimming kinematics objectively. The objective of this study was to evaluate the reliability of inertial sensors placed on equin...
A Familial X-Linked Disorder of Sexual Development in Thoroughbred Horses Associated With a Novel Androgen Receptor Splice-Site Variant.
Animal genetics    April 27, 2026   Volume 57, Issue 3 e70112 doi: 10.1002/age.70112
Letko A, Jagannathan V, Drögemüller C, Lühken G.No abstract available
Membrane and mitochondrial antioxidants improve post-thaw functional quality of stallion spermatozoa.
Animal reproduction science    April 27, 2026   Volume 291 108226 doi: 10.1016/j.anireprosci.2026.108226
Kuželová L, Svoradová A, Vašíček J, Dujíčková L, Vozaf J, Berčík J, Halo M, Halo M, Šmondrková M, Chrenek P.Cryopreservation of stallion semen induces oxidative stress that compromises sperm motility, membrane integrity, and mitochondrial function. Given the high mitochondrial reliance of equine spermatozoa and their susceptibility to oxidative damage, compartment-specific antioxidant strategies may improve post-thaw functional resilience. This study evaluated the effects of astaxanthin, a membrane-active carotenoid, and MitoTEMPO, a mitochondria-targeted superoxide scavenger, on the functional and oxidative status of frozen-thawed stallion spermatozoa. Ejaculates from nine stallions were cryopreser...
The prevalence of Streptococcus equi subsp. equi carriers in the Netherlands.
Equine veterinary journal    April 26, 2026   doi: 10.1002/evj.70177
Houben RMAC, Broens EM, Broekhuizen-Stins MJ, Sloet van Oldruitenborgh-Oosterbaan MM, van Maanen C, Heesterbeek JAP.Streptococcus equi subspecies equi (S. equi) carriers are thought to be important drivers for strangles outbreaks. Limited data are available on the prevalence of carriers in European horse husbandry settings. Objective: To estimate the prevalence of S. equi carriers among apparently healthy adult horses and ponies in the Netherlands. Methods: Cross-sectional survey. Methods: The carrier prevalence in the Netherlands was estimated, detecting carriers by PCR analysis of three repeated nasopharyngeal lavages at weekly intervals. Bayesian methods were used for a true prevalence estimation. Result...
An in vitro collagen gel contraction assay to assess the relaxing effect of potential pharmacological alternatives to oxytetracycline on foals’ tendons.
Scientific reports    April 25, 2026   Volume 16, Issue 1 13412 doi: 10.1038/s41598-026-49449-4
Cardinaux EM, Oltmanns H, Rohn K, Meißner J, Geburek F.Oxytetracycline has been successfully used to treat flexural limb deformities in foals. However, its antimicrobial properties and potential adverse side effects warrant the investigation of alternatives. Inhibition of matrix metalloproteinases (MMPs) or collagen cross-linking have been suggested as possible mechanisms of action. This study assessed the influence of potential alternative substances lacking antimicrobial properties on collagen gel contraction by juvenile equine myofibroblasts in vitro. Myofibroblasts were isolated from forelimb SDFTs and inferior check ligaments from foals eutha...
Anatomical assessment of endodontic debridement challenges in severely diseased equine cheek teeth.
Equine veterinary journal    April 24, 2026   doi: 10.1002/evj.70179
Korsós SA, Josipovic I, Vlaminck L.There is a lack of knowledge on orthograde endodontic techniques in horses and endodontic case selection is mostly empirical currently. Objective: To explore the efficacy of endodontic debridement in severely diseased cheek teeth that are currently deemed unsuitable for endodontic treatment, find where debris is left behind, compare intercanal communications with those described in healthy cheek teeth, explore the effects of endodontic repair mechanisms on endodontic debridement and to investigate the presence of accessory canals and apical deltas in infected parts of the pulp system. Methods:...
ATAC-seq and RNA-seq reveal key genes and pathways regulating lactation in the mammary gland of Yili horses.
BMC genomics    April 22, 2026   doi: 10.1186/s12864-026-12840-6
Liu L, Chen B, Kong M, Tian Y, Ma H, Cao H, Liu W.BACKGROUND: Mammary gland development and lactation are dynamic biological processes governed by coordinated changes in chromatin accessibility and gene expression. RESULTS: We performed paired ATAC-seq and RNA-seq profiling to characterize stage-associated chromatin accessibility and transcriptional programs in the Yili horse mammary gland. Using a conservative overlap-based integration, we prioritized candidate genes showing concordant changes across both layers as hypothesis-generating signals for future functional studies. We sequenced mammary gland tissues from early lactation (S1) and pe...
Complete atrioventricular septal defect in Thoroughbred foals: echocardiographic and postmortem findings.
Journal of equine veterinary science    April 22, 2026   105911 doi: 10.1016/j.jevs.2026.105911
Suzuki T, Kato F, Yamaga T, Tagami M, Tagami M.Complete atrioventricular septal defect (AVSD) is rarely reported in horses. We retrospectively reviewed three Thoroughbred foals (2017-2023) with necropsy-confirmed complete AVSD and summarized their clinical presentation, transthoracic echocardiographic findings, and postmortem anatomy. All foals showed early signs of congenital cardiac disease including weakness, failure to thrive and cardiac murmurs. Echocardiography demonstrated a common atrioventricular junction with a single atrioventricular valve and combined atrial and ventricular septal defects; all were classified as Rastelli type C...
Audio-Based Characterization of Gait Parameters in Mangalarga Marchador, Campolina, and Piquira Horses Using Deep Learning.
Animals : an open access journal from MDPI    April 22, 2026   Volume 16, Issue 9 1283 doi: 10.3390/ani16091283
Freire A, Silva AVD, Patterson Rosa L, Guimarães PHS, Araujo BPG, Silva CAF, Borges LRT, Bertechini AG, Meirelles SLC.The evaluation of biomechanical parameters in four-beat gaited horses remains limited by the subjectiveness and complexity of current standard methods. Through a deep learning approach, we aimed to infer dissociation % using only acoustic signals. A total of 268 audio samples were extracted from publicly available videos featuring three Brazilian horse breeds (Mangalarga Marchador, Campolina, and Piquira) performing marcha batida and marcha picada. Acoustic features, including root mean square energy (RMS), zero-crossing rate (ZCR), and 13 Mel-frequency cepstral coefficients (MFCCs), were extr...
Glanders: Past, Present and Future of a Neglected Zoonosis as a Threat in Veterinary and Human Health.
Veterinary medicine and science    April 21, 2026   Volume 12, Issue 3 e70950 doi: 10.1002/vms3.70950
Kanani Y, Oryan A, Derakhshandeh A.Burkholderia mallei is the causative agent of glanders, a serious zoonotic disease of major concern for both animal and human health. This highly contagious and potentially fatal bacterium is always classified as a biothreat agent. In this review, we covered a comprehensive overview of glanders, including the biology of the pathogen, epidemiology, pathogenesis, diagnosis, control strategies and ongoing eradication programs in Eurasia. We also summarized human case reports, differential diagnosis, treatment and organism's antimicrobial properties. The section on vaccine development is among the...
Holding immature equine oocytes for up to 3 nights prior to ICSI under practical shipping conditions (10-22 °C) is compatible with blastocyst development.
Theriogenology    April 21, 2026   Volume 261 117949 doi: 10.1016/j.theriogenology.2026.117949
Carrera-González C, González Ortega C, Gutiérrez Gutiérrez A, Stefanovski D, Hinrichs K.In vitro embryo production via intracytoplasmic sperm injection (ICSI) is widely used in equine reproductive management. Shipping of immature oocytes allows oocyte recovery for ICSI to be performed by referring veterinarians. However, delays in oocyte shipment are common, and their effect on outcomes is unclear. Shipping is typically done at about 22 °C, but previous studies have shown that holding at 15 °C maintains equine oocyte developmental competence. We hypothesized that lower temperatures may better support oocyte viability if the case that delays occur. Oocytes (n = 664) were rec...
Milk productivity of mares.
Brazilian journal of biology = Revista brasleira de biologia    April 20, 2026   Volume 86 e303508 doi: 10.1590/1519-6984.303508
Asset Z, Rahim K, Kargayeva M.To determine the milk productivity of Kazakh horses of the Adai region in variants of selection and technological indicators of milk for the production of cumus in the conditions of the Mangyshlak peninsula of the Republic of Kazakhstan. The object of the study was the Kazakh horse of the Adai intra-breed type at "Taushyk" LLP (Tupkaragan District, Mangystau Region). Mares with a more pronounced milk production were selected and analyzed . they have investigated the quality of milk for further processing. A characteristic feature of the dynamics of milk sugar content during lactation has been ...
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