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Topic:Veterinary Science

Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
Effect of conditioned medium from equine Wharton’s jelly mesenchymal stem/stromal cells on cool-stored stallion semen.
Research in veterinary science    September 23, 2026   Volume 212 106412 doi: 10.1016/j.rvsc.2026.106412
Khodadadi A, Mislei B, Ortiz-Rodríguez JM, Iacono E, Bucci D, Gagliardi N, Mari G, Merlo B.Artificial insemination in horses largely relies on cooled transported semen, but stallion spermatozoa are highly sensitive to cold stress, limiting storage duration. Mesenchymal stem/stromal cell-derived conditioned medium (CM) has shown protective effects on sperm in other species, yet its impact on equine semen remains unexplored. This study investigated the effects of equine Wharton's jelly-derived MSC-CM (eWJ-MSC-CM), on the quality of cooled stallion semen. Semen from five stallions of proven fertility was extended in INRA96 supplemented with 0, 5, or 15% eWJ-MSC-CM at 5 °C for 0, 24 a...
Phenotypic and Genetic Evaluation of Morphometric and Dynamic Traits in Mangalarga Horses.
Journal of equine veterinary science    September 22, 2026   106153 doi: 10.1016/j.jevs.2026.106153
Alberto GH, Faria RADS, Rodrigues LY, Fiorindo CS, Zucatelle C, Vicente AA, Silva JAIV.Genetic evaluation of morphometric and locomotor traits supports objective selection, avoiding exclusive reliance on subjective methods that may fixate breed traits over functionality or cause undesirable indirect selection. Objective: To evaluate genetic and phenotypic parameters for morphometric and dynamic traits in horses of the Mangalarga breed. Methods: The pedigree included 212,361 animals born between 1919 and 2019. Phenotypic subsets for morphometric and dynamic traits comprised 54,617 and 45,010 horses, respectively, born between 1977 and 2019. Variance components were estimated usin...
Comparison of blind end closure techniques in equine ileum.
Veterinary surgery : VS    September 22, 2026   doi: 10.1111/vsu.70161
Hilliard RL, Prisk A, Wang S, Hansen S, Munsterman A.To determine the difference in bursting pressure (BP), bursting wall tension (BWT), and closure speed between commonly utilized methods of ileal stump closure. Methods: Randomized experimental study. Methods: A total of 71 ileal segments from 18 healthy horses. Methods: Ileum was collected postmortem from 18 horses and divided into four segments. One end of each segment was closed using one of three techniques: Parker-Kerr (PK), gastrointestinal staples (ST), or gastrointestinal staples with a Lembert staple oversew (SO). Closure time was recorded. Control (C) segments were not altered. Segmen...
Myocardial dysfunction associated with low-dose endotoxin administration in adult horses.
Journal of veterinary internal medicine    September 21, 2026   Volume 40, Issue 5 aalag205 doi: 10.1093/jvimsj/aalag205
Williams Louie E, Cercone M, Delvescovo B, Chevalier J, Mitchell K.Horses with endotoxemia have variable myocardial function, but little is known about the severity or persistence of myocardial dysfunction (MD). Objective: Horses with experimentally induced endotoxemia have systolic and diastolic MD. Methods: Twelve systemically healthy horses. Methods: Horses were given Escherichia coli lipopolysaccharide 30 ng/kg over 60 min. Echocardiography and cardiac troponin I (cTnI) measurements were performed at baseline, 1, 3, and 5 h post-endotoxin. Fluid therapy (10 L), polymyxin B (6000 IU/kg), and flunixin meglumine (1.1 mg/kg) were administered intravenou...
Prolyl-Hydroxyproline Promotes Structural Matrix Organization in 3D Equine Tendon Proper and Peritenon Post-Injury Model.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society    September 21, 2026   Volume 44, Issue 9 e70284 doi: 10.1002/jor.70284
Wong AO, Mienaltowski MJ.Tendinopathy of the superficial digital flexor tendon affects horse health and performance, with slow healing and high re-injury rates due to the formation of mechanically weaker scar tissue. Tendons are rich in Type I collagen and may benefit from supplementation with collagen-derived dipeptide prolyl hydroxyproline (H-Pro-Hyp-OH) which improves collagen regeneration and reduces scarring in murine injury models. However, its effects on equine tenocytes in the tendon proper (TP) and peritenon (PERI) under stress are unknown. Three-dimensional fibrin-based TP and PERI constructs were used to ev...
Millimeter‑segmental analysis of individual equine mane hairs by LC/HRMS for revealing chronological drug distribution in anti‑doping investigations.
Forensic science international    September 19, 2026   Volume 389 113144 doi: 10.1016/j.forsciint.2026.113144
Shigematsu R, Osame R, Yokotsuka N, Takemoto S, Minamijima Y, Furukawa T, Hirano-Kodaira M, Yamada M, Leung GN, Taira S.Millimeter‑segmental analysis offers substantially improved temporal resolution over conventional bulk hair testing, yet no validated workflow has been established for equine mane hair. This proof‑of‑concept study presents the first liquid chromatography/high‑resolution mass spectrometry (LC/HRMS)‑based millimeter‑segmental workflow optimized for equine anti‑doping investigations. Mane hairs collected up to six months after a single 50‑mg oral administration of osilodrostat were cut into 2‑mm segments, extracted under standard conditions, and quantified using a fully-validate...
Factors influencing veterinary student interest in equine practice in the United States: A focus group study.
Equine veterinary journal    September 18, 2026   doi: 10.1002/evj.70335
Hothem M, Lestitian C, Burnette A, Tan JY, Graves M, Hunt J, Devine E, Anderson S, Kirkendall K.The number of new veterinary graduates entering equine practice in the United States continues to decline, and more than half leave the field within 5 years. Understanding the perspectives of veterinary students is critical for developing interventions that promote retention and long-term career satisfaction in equine medicine. Objective: To explore what factors influence veterinary student interest in and commitment to equine practice using the Conservation of Resources (COR) Theory as a guiding framework. Methods: Qualitative descriptive study using semi-structured student focus groups. Me...
The Influence of Season on Endocrine Testing in Equids.
The Veterinary clinics of North America. Equine practice    September 17, 2026   Volume 42, Issue 3 605-614 doi: 10.1016/j.cveq.2026.07.006
Lopes A, Durham AE.Endocrine function in equids is strongly influenced by season, with photoperiod as the primary driver and additional modulation by factors such as breed, geography, age, and nutrition. These seasonal effects have important implications for the interpretation of endocrine diagnostic tests. Circannual rhythms influence pituitary activity, resulting in predictable seasonal variation in adrenocorticotropic hormone concentrations and forming the basis for seasonally adjusted diagnostic thresholds for pituitary pars intermedia dysfunction. Seasonal effects on insulin dynamics are less understood but...
Use of a caudal intercostal block in horses undergoing surgery for colic.
Equine veterinary journal    September 15, 2026   doi: 10.1002/evj.70327
Burke MJ, Sheahan BJ, Young KA, Briley JD, Fogle C.In humans and companion animals, regional anaesthesia is used in multimodal analgesic protocols for abdominal wall surgery. Benefits include earlier patient mobilisation and improved pain scores. Regional anaesthetic techniques for horses undergoing abdominal surgery could provide similar benefits and warrant investigation. Objective: To evaluate the safety and efficacy of a caudal intercostal block for abdominal surgery (CIBAS) in horses undergoing surgery for colic. Methods: Blinded, randomised clinical trial. Methods: Horses (n = 22) undergoing exploratory celiotomy for colic were rando...
Context-aware machine learning for lameness side and severity prediction in trotting horses: A proof-of-concept study.
Equine veterinary journal    September 13, 2026   doi: 10.1002/evj.70326
Gérard M, Hanne-Poujade S, Dubois G, Hébert C, Coudry V, Jacquet S, Bertoni L, Tallaj A, Mezghani N, Chateau H.In clinical practice, lameness evaluation often relies on visual assessment under different examination conditions, including ground and figures, and remains subjective. Although objective symmetry indices provide quantitative measures of gait asymmetry, their interpretation across multiple conditions remains challenging because several complementary indices are required and their thresholds vary with context. Objective: To assess the ability of a context-aware machine learning approach to predict lameness side and severity at the trot using trunk-mounted IMUs. Methods: Retrospective observati...
Cryopreservation of Bovine, Equine, and Porcine Sperm.
Methods in molecular biology (Clifton, N.J.)    September 13, 2026   Volume 3060 319-337 doi: 10.1007/978-1-0716-5416-3_11
Oldenhof H, Wolkers WF, Tönißen A, Sieme H.In livestock breeding, cryopreserved semen is routinely used for artificial insemination. Sperm cryopreservation enables long-term storage and transport of insemination doses, thereby securing genetic material for later use. Although specifics may vary, commonly used sperm cryopreservation protocols for domestic species feature similar processing steps as outlined in this chapter. We here describe common procedures for cryopreservation of bull, stallion and boar sperm. This includes preparation and use of the following diluents: TRIS egg yolk extenders (TEY) for bull sperm, ordinary and freezi...
Effects of steaming and storage on microbial quality and nutrient losses of hay.
Journal of equine veterinary science    September 10, 2026   Volume 167 106146 doi: 10.1016/j.jevs.2026.106146
Kummer LK, Schrödl W, Schnabel CL, Schwarzenbolz U, Vervuert I, Lohmann KL.Steaming reduces microbial content of hay but the effect of steaming on nutrient quality and the effects of storage after steaming on microbial content are incompletely known. Objective: It was hypothesized that steamed hay can be stored for up to 24 h without an increase in microbial content and that steaming of hay favors the increase in measurable Maillard reaction products. Methods: Spoiled hay was steamed and then stored in hay nets, buckets or the hay steamer itself under summer and winter climate conditions. Microbial content was assessed by bacterial and fungal culture before and after...
Anesthetic Recovery Following a Midazolam-Containing Co-Induction Protocol in Horses Undergoing Total Intravenous Anesthesia.
Journal of equine veterinary science    September 9, 2026   106144 doi: 10.1016/j.jevs.2026.106144
Claudino JA, Avelar PHS, Rosa MCB, Ferrante M, Souza GP.Anesthetic recovery is a critical phase in horses under field conditions, and induction protocols may influence recovery quality during total intravenous anesthesia. Objective: To compare two anesthetic induction protocols, one including midazolam and the other based solely on xylazine and ketamine, regarding anesthetic induction and recovery quality, total recovery time, and attempts to achieve standing in horses undergoing total intravenous anesthesia. Methods: Twelve healthy male horses older than 2 years were randomly assigned to XC (xylazine 1 mg/kg and ketamine 2 mg/kg) or XCM (xylazine ...
Pelvic Flexure Enterotomy in Horses: Scoping Review and Synthesis of the Literature (1996-2026).
Veterinary sciences    September 9, 2026   Volume 13, Issue 9 930 doi: 10.3390/vetsci13090930
Albanese V, Straticò P, Munsterman A.This scoping review maps the available literature aimed at identifying the most appropriate technique for suturing pelvic flexure enterotomies in horses. A comprehensive search of Web of Science, Google Scholar, and the National Center for Biotechnology Information was conducted for studies published between January 1996 and February 2026, using terms related to pelvic flexure enterotomy and equine patients. From 22,146 records, only five ex vivo studies on healthy equine colon specimens met the inclusion criteria. The studies compared variables such as suturing time, suture pattern, material,...
Comparison of donkey and stallion semen preservation: effects of egg yolk plasma, seminal plasma and extenders on sperm motility, MALDI profiles and fertility.
Reproduction & fertility    September 9, 2026   Volume 7, Issue 3 RAF260059 doi: 10.1530/RAF-26-0059
Petit A, Eloy R, Labas V, Reigner F, Gesbert A, Crampon A, Barrière P, Blard T, Gaude Y, Druart X.The conservation of donkey breeds at risk of extinction requires the development and optimisation of semen preservation and artificial insemination strategies aimed at improving reproductive efficiency, increasing genetic diversity and supporting sustainable population management. The effects of commercial extenders (INRA96, BotuSemen Gold, Beyond), seminal plasma (SP) removal and egg yolk plasma (EYP; 0-10%) supplementation on jack semen stored at 4°C for 24-48 h were evaluated, using stallion semen as a reference. Sperm motility was assessed by computer-assisted sperm analysis (CASA), where...
Influence of Manual Double-Spin Preparation Protocols on the Cellular and Growth Factor Composition of Equine Platelet-Rich Plasma.
Animals : an open access journal from MDPI    September 8, 2026   Volume 16, Issue 18 2824 doi: 10.3390/ani16182824
Del Rincon A, Duggan MJS, Baltrimaite M, O'Kane A, O'Leary JM, Brama PAJ, Kearney C.This prospective, randomised, ex vivo study investigated the effects of manual double-spin centrifugation protocols on the cellular and growth factor composition of equine platelet-rich plasma (PRP). Blood from 20 healthy horses was evaluated in two phases. In Phase A (n = 10), nine first-spin protocols were compared, and two were selected based on platelet enrichment and leukocyte reduction. In Phase B (n = 10), these were combined with nine second-spin conditions, generating 18 PRP preparations. Platelet (PLT), leukocyte (WBC) and erythrocyte (RBC) concentrations and PDGF-BB and TGF-β conce...
Morphological Characterization of Turkish Ambling Horses.
Animals : an open access journal from MDPI    September 8, 2026   Volume 16, Issue 18 2813 doi: 10.3390/ani16182813
Bayramoğlu B, Tuna YT.Morphometric information on ambling horses raised in Türkiye remains limited. This study aimed to characterize the morphometric traits of ambling horses according to breeding origin, age, and sex and to provide baseline phenotypic information on the studied population. A total of 102 naturally ambling horses were evaluated, including indigenous horses raised in Bursa ( = 28), indigenous horses raised in Nazilli, Aydın Province ( = 65), and Afghan-origin horses raised in Bursa ( = 9). Morphometric measurements of the head, body, and limbs were recorded, and the effects of breeding origin, age...
Evaluation of a new immunoassay for measuring equine serum insulin and plasma adrenocorticotrophic hormone: A method comparison study.
Equine veterinary journal    September 6, 2026   doi: 10.1002/evj.70329
Menzies-Gow NJ, Cosin P, Guyot V.Insulin dysregulation (ID) and pituitary pars intermedia dysfunction (PPID) are common equine endocrinopathies detected by measurement of circulating insulin and adrenocorticotrophic hormone (ACTH) concentrations. Different assays may yield variable results, limiting comparability. Objective: To compare immunoassays developed specifically to measure equine insulin (VIDAS® Equine Insulin; enzyme-linked fluorescent assay [ELFA]) and equine ACTH (VIDAS® Equine ACTH; ELFA) with results obtained from a chemiluminescence immunoassay (CLIA) measured using IMMULITE 2000XPi, a chemiluminescence enzym...
Anatomy and Ultrasound-Guided Injection of the Deep Branch of the Lateral Palmar Nerve in the Horse: A Cadaveric Study.
Veterinary and comparative orthopaedics and traumatology : V.C.O.T    September 2, 2026   doi: 10.1055/a-2944-1706
Corraretti G, Enriques C, Zani DD, Gustafsson KEV.Proximal suspensory ligament (PSL) injuries are a common cause of lameness in horses. Localization of pain in the proximal metacarpal and carpal region remains challenging. Current diagnostic analgesic techniques are not PSL-specific. The aim of this study was to investigate the nerve supply of the PSL, with particular focus on the deep branch of the lateral palmar nerve (DBLPaN), and to develop a landmark-based, ultrasound-guided injection technique targeting this nerve. Unassigned: Eighteen limbs from euthanized horses were dissected to describe the anatomy of the DBLPaN and branches, measur...
Correction: Villalba et al. Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications. Viruses 2024, 16, 470.
Viruses    September 2, 2026   Volume 18, Issue 9 963 doi: 10.3390/v18090963
Villalba R, Tena-Tomás C, Ruano MJ, Valero-Lorenzo M, López-Herranz A, Cano-Gómez C, Agüero M.In the original publication [...].
Diagnostic utility of trigeminocervical reflex and quantitative sensory testing in horses with trigeminal-mediated headshaking.
Journal of veterinary internal medicine    September 1, 2026   Volume 40, Issue 5 aalag199 doi: 10.1093/jvimsj/aalag199
Käfer-Karrer MJ, Spadavecchia C, de Preux M, Unger L.Assessment of trigeminal nerve (TN) function in horses with trigeminal-mediated headshaking (TMHS) using trigeminocervical reflex (TCR) and quantitative sensory testing (QST) has not been investigated. Objective: Horses with TMHS show lower TCR and QST thresholds than controls. A subset shows left-to-right differences. Methods: Privately owned horses with TMHS (n = 12) and secondary headshaking (sHS; n = 4), from a referral hospital. Headshaking-free controls (n = 14), from the hospital's teaching herd or privately owned. Methods: Prospective case-control-study. In unsedated horses, TCR ...
Electrocardiographic recording in horses at rest: a concordance analysis of two methods.
Veterinary research communications    September 1, 2026   Volume 50, Issue 6 545 doi: 10.1007/s11259-026-11486-9
Cardiac arrhythmias are frequently detected during routine auscultation in athletic horses. Assessment of their clinical significance requires electrocardiography (ECG) to analyze waveforms and intervals. In recent years, portable ECG devices have been introduced to simplify electrocardiographic examination, offering advantages such as ease of use and the ability to review results via a smartphone. This study evaluated the utility and accuracy of a portable ECG device compared with a standard electrocardiograph. Simultaneous ECG recordings were obtained from 28 healthy adult horses at rest usi...
Synthesis and Characterization of 2-(1-Hydroxyethyl)Promazine Sulfoxide-d4 HCl as an Internal Standard for the Accurate Quantitation of 2-(1-Hydroxyethyl)Promazine Sulfoxide in Equine Urine.
Journal of labelled compounds & radiopharmaceuticals    September 1, 2026   Volume 69, Issue 10-11 e70036 doi: 10.1002/jlcr.70036
Holmes JC, Smith BC, Awuah SG, Eisenberg R, Lehner AF, Fenger CK, Maylin GA, Brewer K, Tobin T.Acetylpromazine, 1-{10-[3-(dimethylamino)propyl]-10H-phenothiazin-2-yl}ethenone, CHNOS, 326.46 g·mol is a phenothiazine derivative at one time used in human medicine as an antipsychotic medication but now predominantly used in veterinary medicine as a sedative/tranquilizer and referred to as acepromazine. In performance horses its use is regulated by using a 10 ng/mL threshold for the major urinary metabolite 2-(1-hydroxyethyl) promazine-sulfoxide (HEPS) in equine urine. To enable accurate quantitation of HEPS in equine urine we have synthesized and purified hydroxyethylpromazine sulfoxid...
Multiple Probiotic Lactic Acid Bacteria with Marked Cholesterol-Lowering Effects Were Discovered in Equine Colostrum.
International journal of molecular sciences    August 31, 2026   Volume 27, Issue 17 7816 doi: 10.3390/ijms27177816
Liu X, Hudayibieergen K, Yeerjiang B, Manaer T, Nabi X.Xinjiang ranks among the key domestication origins of horses. Local nomadic ethnic groups have a long-standing tradition of consuming equine dairy products, which endows mare-derived dairy matrices with abundant, distinctive and scarce probiotic resources. Equine colostrum is rich in immunoglobulins and growth factors; nevertheless, its pharmacological activities and micro-ecological composition remain poorly characterized. This study aimed to isolate and screen native strains with cholesterol-lowering potential from Xinjiang equine colostrum and conduct a preliminary investigation into their ...
Spatial and temporal radiation exposure during equine bone scintigraphy: Implications for occupational safety and post-scintigraphy isolation.
Equine veterinary journal    August 26, 2026   doi: 10.1002/evj.70321
Mageed M, Dyab S, Przewoźny M.Bone scintigraphy is a valuable diagnostic modality in equine orthopaedics, but it is associated with occupational radiation exposure and regulatory requirements for post-procedural isolation. Objective: To quantify radiation dose rates emitted from horses undergoing bone scintigraphy, to evaluate the effects of distance (spatial) and time (temporal) on radiation exposure, and to derive evidence-based recommendations for post-scintigraphy isolation periods. Methods: Prospective, cross-sectional study. Methods: Twenty warmblood horses underwent musculoskeletal scintigraphy following intravenous...
An atypical extrapulmonary presentation of rhodococcus equi infection in a foal: a case report.
Veterinary research communications    August 26, 2026   Volume 50, Issue 6 532 doi: 10.1007/s11259-026-11469-w
Tamulionytė-Skėrė R, Mickevičienė I, Gruodytė R, Paulavičiūtė B, Šiugždinienė R, Hinksman J, Urbonavičiūtė A, Driukė MU.Rhodococcus equi infection in foals is primarily associated with pulmonary disease but may also present with extrapulmonary manifestations (Giguère et al. 2011; Reuss et al. 2009). Hemolytic anemia is a rare and poorly described complication, with only a single previous report of presumed immune-mediated hemolytic anemia in foals affected by R. equi. This case report describes the clinical presentation, diagnostic findings, disease progression, and postmortem examination of a 4-month-old Holsteiner foal with confirmed R. equi infection and severe anemia consistent with a presumed immune-media...
Computed Tomographic Lesion Descriptors in Foals With Hematogenous Osteomyelitis: Interobserver Agreement and Association With Outcome. Gustafsson K, Longo M, Vanderperren K, Haardt H, Haspeslagh M, Stas E, De Zani D, Buyck C, Robert M, Zani DD.Computed tomography (CT) is increasingly used to diagnose osteomyelitis in foals, but the prognostic value and interobserver reproducibility of specific CT lesion descriptors remain unclear. This multicenter retrospective study aimed to characterize CT features of hematogenous osteomyelitis in foals using a structured radiologic feature set, assess interobserver agreement, and evaluate associations between CT descriptors and survival to hospital discharge and long-term survival. We hypothesized that CT features considered aggressive, including a long transition zone, permeative or moth-eaten o...
Non-coding RNA profiles associated with equine osteoarthritis: a systematic review.
Frontiers in veterinary science    August 26, 2026   Volume 13 1917722 doi: 10.3389/fvets.2026.1917722
Khaliji E, Wong TS, Barker CM, Chen S, Cassano JM.Non-coding RNAs (ncRNAs) play a role in the pathogenesis of osteoarthritis (OA) by regulating gene expression related to inflammation, chondrocyte apoptosis, and extracellular matrix degradation. In horses, OA is a leading cause of lameness and poor performance, with no current treatments halting disease progression and no clear biomarker available. Although some studies have investigated expression profiles of ncRNAs in equine OA, there is a need to systematically review previous efforts and synthesize information to identify potential ncRNAs to target in future research. Unassigned: The sear...
Evidence-based interpretation of uterine cultures in mares: Linking bacterial growth patterns to endometrial inflammation.
PloS one    August 25, 2026   Volume 21, Issue 8 e0356822 doi: 10.1371/journal.pone.0356822
Yáñez U, Krupa M, Gibbons J, Storme J, Lewis N, Pascottini OB.We evaluated uterine bacterial growth patterns and their association with endometrial inflammation (EI) in equine samples collected using uterine swabs (US) or low-volume uterine lavage (UL). A database including 1,545 US and 2,066 UL with cytological and bacteriological results was retrospectively analysed. Endometrial inflammation was defined as ≥2 polymorphonuclear cells per high-power field, and bacterial culture was considered positive when aerobic growth occurred within 48 h, with the number of bacterial isolates per sample recorded (0, 1, 2, or ≥3). Data were analysed using generali...
Sixty-day oral antioxidant supplementation enhances sperm morphology and seminal plasma antioxidant capacity in stallions.
Journal of animal science    August 25, 2026   Volume 104 skag265 doi: 10.1093/jas/skag265
Del Prete C, Longobardi C, Longobardi V, Piscopo F, Ciarcia R, Cocchia N, Pasolini MP, Cutrignelli MI, Vastolo A.While dietary supplementation with antioxidants has been identified as an effective strategy for mitigating oxidative stress (OS) damage in stallion sperm, specific protocols and the duration of its efficacy remain poorly defined. This study aimed to evaluate the effects of a 60-d dietary supplementation with a commercial antioxidant combination on semen quality and OS biomarkers in stallions. Stallions were divided into two groups: the control group (n = 5; CTRL) received 30 g/500 kg of placebo while the treated group (n = 5; OXY) received 30 g/500 kg of Oxyliver daily for 60â...