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Musculoskeletal Assessment of Forelimbs and Hindlimbs in Mounted Police Horses using Infrared Thermography.
Journal of equine veterinary science    September 13, 2026   106149 doi: 10.1016/j.jevs.2026.106149
da Silva BBM, Fialho DVC, da Silva GS, Campos LB, Caldara FR, Braga CS, Weber FL, Lima ABM, Rudek LS, Veloso AS, Sousa MS, da Silva MA.Infrared thermography (IRT) has become established as a complementary tool in equine veterinary medicine, allowing for the early detection of thermal changes associated with musculoskeletal inflammatory processes. Objective: This study aimed to apply infrared thermography to characterize the surface thermal profile of the forelimbs and hindlimbs of mounted police horses, considering clinical classification, anatomical region, body side, and repeated evaluations over time. Methods: The research was conducted at the Military Police Cavalry in Manaus (AM), Brazil, involving 20 adult horses submit...
Cryopreservation of Bovine, Equine, and Porcine Sperm.
Methods in molecular biology (Clifton, N.J.)    September 13, 2026   Volume 3060 319-337 doi: 10.1007/978-1-0716-5416-3_11
Oldenhof H, Wolkers WF, Tönißen A, Sieme H.In livestock breeding, cryopreserved semen is routinely used for artificial insemination. Sperm cryopreservation enables long-term storage and transport of insemination doses, thereby securing genetic material for later use. Although specifics may vary, commonly used sperm cryopreservation protocols for domestic species feature similar processing steps as outlined in this chapter. We here describe common procedures for cryopreservation of bull, stallion and boar sperm. This includes preparation and use of the following diluents: TRIS egg yolk extenders (TEY) for bull sperm, ordinary and freezi...
Historical, clinical and nasal endoscopic findings in 1600 cases of equine sinonasal disease and associations with case outcome.
Equine veterinary journal    September 13, 2026   doi: 10.1002/evj.70306
Dixon P, Parente E, Barnett T, Morgan R, O'Leary JM, Vlaminck L, Talbot A, Gillen A, McGinley T, Brennan M, Tremaine H, Polaris E, Dobbs E....No large study has described the signalment, pre-referral management or clinical and nasal endoscopic findings in equine sinonasal disease. Objective: To explore associations between case outcome and signalment, pre-referral treatments, clinical and nasal endoscopic variables in sinonasal disease. Methods: Retrospective case series. Methods: Data were collected from medical records of 19 equine hospitals on sinonasal disease cases they had examined and which had undergone computed tomographic (CT) imaging. Case outcome was obtained from clinical records and/or by contacting clients and referri...
Chronic Endometrial Degeneration (CED): an international expert recommendation to replace the term ‘endometrosis’ in mares.
Theriogenology    September 12, 2026   Volume 267 118185 doi: 10.1016/j.theriogenology.2026.118185
England GC, Aurich C, Fingerhood S, Gilbert RO, Newcombe J, Paccamonti DL, Troedsson MH, Ferreira-Dias GM, de Brot S, Freeman SL.The term 'endometrosis', coined in 1992 to describe chronic degenerative changes of the equine endometrium, is descriptively imprecise and readily confused with 'endometritis' and the human gynaecological condition 'endometriosis'. A modified four-round electronic Delphi (e-Delphi) process was conducted with an international panel of eight specialists to achieve consensus on a replacement term. Fifteen candidate terms were evaluated in Round 1 using a 7-point Likert scale and preference rankings; terms were retained if >50% of respondents scored ≥4/7. Round 2 (Refinement) evaluated shortl...
Coprological assessment of gastrointestinal helminths in Arabian Thoroughbred horses housed in a stable in the Constantine region, Algeria.
Helminthologia    September 11, 2026   Volume 63, Issue 2 167-173 doi: 10.2478/helm-2026-0019
Zeghilet N, Boultif L, Djemai S, Bouchoucha B.This study assesses the gastrointestinal parasitological status of Arabian Thoroughbred horses at the Eastern Equestrian Club in Constantine (Algeria). In this semi-arid region, epidemiological data on equine parasitoses are lacking. Twenty fecal samples were analyzed using qualitative (flotation) and quantitative (McMaster) methods, complemented by morphological identification of parasitic eggs. The results reveal an overall prevalence of 50 %, with species diversity dominated by strongyles (60 % of positive cases), followed by (40 %), spp. (30 %), (20 %), and spp. (10 %). The excretion i...
Computed tomographic findings in 1600 cases of equine sinonasal disease and associations with case outcome.
Equine veterinary journal    September 11, 2026   doi: 10.1002/evj.70307
Dixon P, Morgan R, Parente E, Barnett T, O'Leary JM, Vlaminck L, Talbot A, Gillen A, McGinley T, Brennan M, Tremaine H, Pollaris E, Dobbs E....No study has described the computed tomographic (CT) sinonasal findings in a large cohort of referred sinonasal disease cases. Objective: To report the CT imaging findings in equine sinonasal disease and examine associations between case outcome and CT findings. Methods: Retrospective case series. Methods: Data were collected from medical records from 19 equine hospitals on sinonasal disease cases which had undergone CT imaging. Case outcome was obtained from clinical records, and/or by contacting clients and referring veterinarians. Multivariable logistic regression analysis was used to exami...
Stable Beginnings – A call to transform methods of weaning horses.
Journal of equine veterinary science    September 11, 2026   106148 doi: 10.1016/j.jevs.2026.106148
Dwyer J, Freeman SL, Hill B, Roshier AL.There are currently a range of different weaning methods used in the UK, with no clear consensus or guidance to support the optimal welfare of both mares and foals during and post weaning. Objective: To explore welfare charity professionals' perceptions of approaches to weaning horses in the UK. Methods: Participants from welfare charities with experience in management, and welfare of horses from birth to death were recruited. They reviewed videos of weaning approaches and a chart of behavioural welfare indicators. They then took part in discussions on methods of weaning, considering their opi...
Beyond Body Condition Score: A Proposed Interspecies Framework for Tissue-by-Tissue Nutritional Assessment in Veterinary Practice.
Veterinary sciences    September 10, 2026   Volume 13, Issue 9 938 doi: 10.3390/vetsci13090938
Cappai MG.In clinical veterinary medicine, the Body Condition Score (BCS) serves as a validated, semi-quantitative estimation of subcutaneous adipose reserves, yet it cannot by itself detect systemic nutrient deficiencies or excesses that do not necessarily imply energy storage. Human clinical dietetics addressed an analogous diagnostic gap through the Nutrition-Focused Physical Examination (NFPE), but veterinary medicine still lacks a unified, multi-species physical examination sequence for nutritional screening. This narrative review synthesizes the comparative and clinical nutrition literature into a...
Limited evidence for exposure of horses in the United Kingdom to influenza D virus.
Journal of equine veterinary science    September 10, 2026   Volume 167 106141 doi: 10.1016/j.jevs.2026.106141
Wu G, Norman F, Maina MM, Kuchipudi SV, Nissly RH, Scott SD, Temperton N, Dunham SP, Daly JM.Since it was first detected in 2011, antibodies to influenza D virus (IDV) have been found in several species, although cattle are the major host. In cattle, IDV is typically associated with mild respiratory signs, but it is clinically significant as a component of bovine respiratory disease complex. Although antibodies to IDV have been detected in equids, no association of IDV with naturally occurring respiratory disease in horses has yet been demonstrated. Objective: The aim of this study was to determine whether IDV may be a causal agent of equine respiratory disease in the UK. Methods: Nas...
Equine-based community service offered through horse breed association youth programs: Parent and community partner perceptions of participation, benefits, and program growth.
Journal of equine veterinary science    September 10, 2026   106142 doi: 10.1016/j.jevs.2026.106142
Culwell R, Nicodemus MC, Jousan D, Irvin L, Williams T, Hall B.Equine-based community service activities offered through horse breed association youth programs provide opportunities for youth to engage in volunteerism while interacting with horses. Although these programs are widely available, research examining stakeholder perceptions remains limited. Objective: To examine parent and community partner perceptions of equine-based community service activities offered through horse breed association youth programs, including perceived benefits, participation patterns, recruitment, implementation challenges, and opportunities for program development. Methods...
Effects of steaming and storage on microbial quality and nutrient losses of hay.
Journal of equine veterinary science    September 10, 2026   Volume 167 106146 doi: 10.1016/j.jevs.2026.106146
Kummer LK, Schrödl W, Schnabel CL, Schwarzenbolz U, Vervuert I, Lohmann KL.Steaming reduces microbial content of hay but the effect of steaming on nutrient quality and the effects of storage after steaming on microbial content are incompletely known. Objective: It was hypothesized that steamed hay can be stored for up to 24 h without an increase in microbial content and that steaming of hay favors the increase in measurable Maillard reaction products. Methods: Spoiled hay was steamed and then stored in hay nets, buckets or the hay steamer itself under summer and winter climate conditions. Microbial content was assessed by bacterial and fungal culture before and after...
Cyathostomin species richness and alpha diversity at egg reappearance following fecal egg count-based selective ivermectin treatment.
Veterinary parasitology    September 10, 2026   Volume 348 110923 doi: 10.1016/j.vetpar.2026.110923
Halvarsson P, Hedberg-Alm Y, Tydén E.Cyathostomin nematodes are ubiquitous in grazing horses. The overuse of anthelmintics has led to increasing problems with drug resistance to benzimidazoles and pyrantel. Although macrocyclic lactones (ML), such as ivermectin, generally retain the highest efficacy, reduced ML efficacy has increasingly been reported. A shortened egg reappearance period (ERP) is frequently used as an indicator of reduced susceptibility. However, the species-specific response dynamics after ivermectin treatment are inadequately characterized in cyathostomins. The objective of this study was to compare the cyathost...
Comparison of donkey and stallion semen preservation: effects of egg yolk plasma, seminal plasma and extenders on sperm motility, MALDI profiles and fertility.
Reproduction & fertility    September 9, 2026   Volume 7, Issue 3 RAF260059 doi: 10.1530/RAF-26-0059
Petit A, Eloy R, Labas V, Reigner F, Gesbert A, Crampon A, Barrière P, Blard T, Gaude Y, Druart X.The conservation of donkey breeds at risk of extinction requires the development and optimisation of semen preservation and artificial insemination strategies aimed at improving reproductive efficiency, increasing genetic diversity and supporting sustainable population management. The effects of commercial extenders (INRA96, BotuSemen Gold, Beyond), seminal plasma (SP) removal and egg yolk plasma (EYP; 0-10%) supplementation on jack semen stored at 4°C for 24-48 h were evaluated, using stallion semen as a reference. Sperm motility was assessed by computer-assisted sperm analysis (CASA), where...
Associations between treatments and response to treatments from a large multicentre study of equine sinonasal disease.
Equine veterinary journal    September 9, 2026   doi: 10.1002/evj.70319
Dixon P, Barnett T, Parente E, Morgan R, O'Leary JM, Vlaminck L, Talbot A, Gillen A, McGinley T, Brennan M, Tremaine H, Pollaris E, Dobbs E....No large study has examined the treatment and response to treatment of horses with sinonasal disease confirmed by computed tomography (CT). Objective: To report the treatment and response to treatment of referred cases of equine sinonasal disease. To explore associations between case outcome and clinical and endoscopic features, CT findings, disease subtypes, and treatments in a multivariable model. Methods: Retrospective case series. Methods: Data were collected from medical records from 19 equine hospitals on cases of sinonasal disease, all of which underwent CT imaging. Case outcome was obt...
Management practices and human-horse relationships are associated with horse personality traits.
Royal Society open science    September 9, 2026   Volume 13, Issue 9 260116 doi: 10.1098/rsos.260116
Viitanen A, Ståhl A, Salonen MK, Liehrmann O.The human-horse relationship has evolved through centuries of domestication, shaping how horses are managed and interact with people. Horses show interindividual behavioural differences that constitute personality, with implications for welfare, management and human-horse interactions. Although equine personality has a genetic component, environmental and relational factors may influence its expression, yet effects of everyday management practices remain poorly understood. This study examined associations between owner-rated horse personality traits and human-controlled environmental and relat...
Single-cell sequencing reveals synovial fluid γδ T-cell expansion in equine experimental osteoarthritis.
American journal of veterinary research    September 9, 2026   1-15 doi: 10.2460/ajvr.26.06.0272
Ammons D, Chow L, Goodrich L, Barrett M, Nelson B, Impastato R, Stoneback JW, Dow S, Pezzanite LM.Define temporal cellular changes following joint injury using single-cell RNA sequencing in experimental equine posttraumatic osteoarthritis (PTOA). Unassigned: PTOA was induced in 4 Quarter Horses (3 to 5 years) via carpal osteochondral fragmentation and high-speed treadmill exercise. Synovial fluid (SF) cells and synovium were sampled over 18 weeks (November 2023 to April 2024). Single-cell suspensions were processed (10x Genomics Chromium iX), then aligned to the equine genome (Cell Ranger). Downstream analysis was completed in the R Seurat package. Differential gene expression (log2[fold c...
Anesthetic Recovery Following a Midazolam-Containing Co-Induction Protocol in Horses Undergoing Total Intravenous Anesthesia.
Journal of equine veterinary science    September 9, 2026   106144 doi: 10.1016/j.jevs.2026.106144
Claudino JA, Avelar PHS, Rosa MCB, Ferrante M, Souza GP.Anesthetic recovery is a critical phase in horses under field conditions, and induction protocols may influence recovery quality during total intravenous anesthesia. Objective: To compare two anesthetic induction protocols, one including midazolam and the other based solely on xylazine and ketamine, regarding anesthetic induction and recovery quality, total recovery time, and attempts to achieve standing in horses undergoing total intravenous anesthesia. Methods: Twelve healthy male horses older than 2 years were randomly assigned to XC (xylazine 1 mg/kg and ketamine 2 mg/kg) or XCM (xylazine ...
Gut Microbiota as a Molecular Regulator of Mineral Homeostasis in Horses: Mechanisms and Future Perspectives.
International journal of molecular sciences    September 9, 2026   Volume 27, Issue 18 8015 doi: 10.3390/ijms27188015
Sajid QUA, Asghar MU, Wróblewska P, Król B, Korczyński M.Mineral homeostasis in horses is traditionally attributed to the coordinated regulation of the intestine, kidneys, skeleton, and endocrine system. Emerging evidence suggests the gut microbiota also shapes this process by modifying the intestinal environment where mineral absorption occurs. Despite the growing interest in host-microbiota interactions, the mechanisms linking the equine gut microbiota to mineral metabolism remain poorly understood and have not been comprehensively synthesized. This review integrates current knowledge of equine gastrointestinal physiology, gut microbial ecology, a...
Pelvic Flexure Enterotomy in Horses: Scoping Review and Synthesis of the Literature (1996-2026).
Veterinary sciences    September 9, 2026   Volume 13, Issue 9 930 doi: 10.3390/vetsci13090930
Albanese V, Straticò P, Munsterman A.This scoping review maps the available literature aimed at identifying the most appropriate technique for suturing pelvic flexure enterotomies in horses. A comprehensive search of Web of Science, Google Scholar, and the National Center for Biotechnology Information was conducted for studies published between January 1996 and February 2026, using terms related to pelvic flexure enterotomy and equine patients. From 22,146 records, only five ex vivo studies on healthy equine colon specimens met the inclusion criteria. The studies compared variables such as suturing time, suture pattern, material,...
Evaluation of tilapia skin (Oreochromis niloticus) frozen, frog skin (Rana catesbeiana), and tilapia skin (Oreochromis niloticus) sterilized with gamma rays dressings, in equine skin wound healing.
Frontiers in pharmacology    September 8, 2026   Volume 17 1896365 doi: 10.3389/fphar.2026.1896365
Miranda RPDR, de Miranda BA, Aranha AB, Gomes Ramos MFB, Oliveira EP, Dallago BSL, Adorno J, Macêdo IL, Castro MB, Campebell RC.Skin wounds are common in horses due to species-specific behavioral responses. Equine skin morphology, vascularization, and inflammatory responses to wounds are comparable to those in humans, supporting the translational relevance of equine wound-healing studies. Investigating biomaterials such as tilapia and frog skin may improve wound management. This study demonstrated that sterilized tilapia and frog skin exhibit promising wound-healing properties. Unassigned: Macroscopic, microscopic, and thermographic aspects of second-intention wound healing were compared in six horses with surgically i...
Diagnosed causes of sinonasal disease in 1600 horses and associations with clinical signs, computed tomographic findings and case outcome.
Equine veterinary journal    September 8, 2026   doi: 10.1002/evj.70308
Dixon P, Barnett T, Parente E, Morgan R, O'Leary JM, Vlaminck L, Talbot A, Gillen A, McGinley T, Brennan M, Tremaine H, Polaris E, Dobbs E....The diagnosis of equine sinonasal disorders has been greatly enhanced by using computed tomographic (CT) imaging. Objective: To explore associations between specific diagnoses and clinical and CT findings and between specific diagnoses and case outcome in equine sinonasal disease. Methods: Retrospective case series. Methods: Data were collected from medical records from 19 equine hospitals on cases of sinonasal disease, all of which underwent CT imaging. Case outcome was obtained from clinical records, and/or by contacting clients and referring veterinarians. Logistic regression analysis was u...
Exhaled breath condensate in horses: a first microbiological insight.
Journal of equine veterinary science    September 8, 2026   106139 doi: 10.1016/j.jevs.2026.106139
Santi TF, Nogara MF, Valente MLVM, Weber SH, Oliveira TM, Bogossian PM, Michelotto PV.Exhaled breath condensate (EBC) is a non-invasive method for obtaining samples reflecting the airway lining fluid; however, its microbiological composition remains poorly characterized. Objective: To perform an exploratory microbiological comparison of EBC in clinically healthy horses maintained under different environmental conditions. Methods: An observational study included 30 mares equally distributed into two groups: horses housed in stalls (Racetrack group) and horses maintained under pasture conditions (Farm group). EBC samples were collected using a validated device developed by the re...
Use of Sildenafil Citrate for the Treatment of Erectile Dysfunction in Stallions: First Case Reports.
Reproduction in domestic animals = Zuchthygiene    September 8, 2026   Volume 61, Issue 9 e70318 doi: 10.1111/rda.70318
Cavalero TMS, Papa FO, Monteiro GA, Papa PM, Hartwig FP, Freitas-Dell'Aqua CP, Alvarenga MA, Freitas MSE, Sancler-Silva YFR.Pharmacological aids can be useful alternatives to enhance penile erection and/or improve ejaculatory function, thereby prolonging the reproductive life of stallions with ejaculatory disorders. This study aimed to evaluate the effects of sildenafil citrate in the treatment of erectile dysfunction and on semen quality in two aged stallions, a Quarter Horse (18 years) and a Mangalarga Marchador (25 years), presented to the Veterinary Hospital of FMVZ-UNESP, Botucatu, SP, Brazil. The treatment consisted of oral administration of sildenafil citrate at 0.2 mg/kg. Thirty minutes after administ...
Morphological Characterization of Turkish Ambling Horses.
Animals : an open access journal from MDPI    September 8, 2026   Volume 16, Issue 18 2813 doi: 10.3390/ani16182813
Bayramoğlu B, Tuna YT.Morphometric information on ambling horses raised in Türkiye remains limited. This study aimed to characterize the morphometric traits of ambling horses according to breeding origin, age, and sex and to provide baseline phenotypic information on the studied population. A total of 102 naturally ambling horses were evaluated, including indigenous horses raised in Bursa ( = 28), indigenous horses raised in Nazilli, Aydın Province ( = 65), and Afghan-origin horses raised in Bursa ( = 9). Morphometric measurements of the head, body, and limbs were recorded, and the effects of breeding origin, age...
The effect of macrophage secretome on equine endometrial fibroblast transcriptomic and functional characteristics.
Theriogenology    September 8, 2026   Volume 267 118177 doi: 10.1016/j.theriogenology.2026.118177
Słyszewska M, Sadowska A, Tobolski D, Piotrowska-Tomala K, Ferreira-Dias G, Szóstek-Mioduchowska A.Endometrial fibrosis is a main hallmark of endometrosis, a degenerative condition of the equine endometrium. Fibrosis, is characterized by the excessive accumulation of extracellular matrix (ECM) components. Current research highlights the crucial role of MΦ in activating fibroblasts in the development of fibrotic processes. However, the specific actions of distinct MΦ subpopulations on equine endometrial fibroblasts remain to be elucidated. Thus, this study aimed to investigate the long-term effect of the secretome of equine in vitro-generated monocyte-derived (eqMD)-MΦ (pro-inflammatory M...
Promises and Perspectives of Mesenchymal Stromal Cells in Companion and Farm Animals.
International journal of molecular sciences    September 8, 2026   Volume 27, Issue 18 7978 doi: 10.3390/ijms27187978
Talluri TR, Nag P, Rathore NS, Kues WA, Kumar D, Selokar N, Bajwa KK, Ansari MM, Bhattacharya TK.Mesenchymal stromal cells (MSCs) are heterogeneous, multipotent cell populations that have become one of the most extensively investigated cell types in veterinary regenerative medicine. They can be isolated from a wide range of adult and perinatal tissues and have attracted considerable interest because of their immunomodulatory properties, broad secretory activity, and potential to promote tissue repair. However, increasing evidence indicates that MSCs should not be regarded as a uniform therapeutic product, as their biological behaviour is influenced by tissue source, donor characteristics,...
Influence of Manual Double-Spin Preparation Protocols on the Cellular and Growth Factor Composition of Equine Platelet-Rich Plasma.
Animals : an open access journal from MDPI    September 8, 2026   Volume 16, Issue 18 2824 doi: 10.3390/ani16182824
Del Rincon A, Duggan MJS, Baltrimaite M, O'Kane A, O'Leary JM, Brama PAJ, Kearney C.This prospective, randomised, ex vivo study investigated the effects of manual double-spin centrifugation protocols on the cellular and growth factor composition of equine platelet-rich plasma (PRP). Blood from 20 healthy horses was evaluated in two phases. In Phase A (n = 10), nine first-spin protocols were compared, and two were selected based on platelet enrichment and leukocyte reduction. In Phase B (n = 10), these were combined with nine second-spin conditions, generating 18 PRP preparations. Platelet (PLT), leukocyte (WBC) and erythrocyte (RBC) concentrations and PDGF-BB and TGF-β conce...
Differential rotational and translational movements along the thoraco-lumbo-sacral spine of load and non-load pulling military and police horses walking and trotting on hard and soft surfaces.
BMC veterinary research    September 8, 2026   Volume 22, Issue 1 559 doi: 10.1186/s12917-026-05804-1
Horan K, Warner SE, Day P, Housby-Skeggs N, Lane-Ley E, Telfer H, Berner D.To improve detection of equine back-related disorders, it is important to establish how movement dynamics in horses' thoraco-lumbo-sacral spine are normally affected by intrinsic and extrinsic factors. This cross-sectional study aimed to quantify how discipline, gait, and surface impact differential rotational and translational movements along the backs of 107 horses from the military and police forces. The horses were walked and trotted in-hand in a straight-line over hard and soft surfaces. During the exercises, nine inertial sensors (XSens MTw) were mounted to the poll, withers, thoracic ve...
Thermographic assessment of surface temperature distribution and agreement between a thermographic camera, infrared thermometer, and rectal temperature in exercising horses under tropical climate conditions.
Veterinary world    September 7, 2026   Volume 19, Issue 9 3899-3914 doi: 10.14202/vetworld.2026.3899-3914
Pongsumpun J, Isawirodom A, Sangsasithorn P, Wong-Aree K, Satumay N, Yamsakul P, Pongkan W, Rungsri P, Lampang KN.Infrared thermography has become an increasingly valuable non-invasive imaging technique for monitoring physiological status and detecting abnormalities in horses. However, its application under tropical environmental conditions remains insufficiently investigated, particularly regarding the influence of ambient conditions on measurement accuracy and agreement with conventional temperature assessment methods. This study aimed to evaluate the agreement among a thermographic camera (TC), infrared thermometer (IR), and rectal temperature (RT), characterize body surface temperature distribution be...
Transcriptome Sequencing and Differential Expression Analysis of Ovaries from Kazakh Mares During Seasonal Quiescence and Estrus Activation.
Biology    September 7, 2026   Volume 15, Issue 17 1566 doi: 10.3390/biology15171566
Zhou Y, Ren W, Zeng Y, Wang J, Meng J, Yao X, Zhai M.Kazakh horses are typical seasonal breeders, and ovarian activity is markedly reduced during the non-breeding period. Hormonal stimulation can induce ovarian functional responses during seasonal anestrus; however, the early ovarian transcriptional changes associated with this response remain incompletely understood. In this study, ovarian transcriptome sequencing was performed in 12 Kazakh mares examined during the seasonal anestrous period. The mares were classified into a non-hormonally stimulated seasonal quiescent group (DB, = 6) and a hormone-induced ovarian activation group (DY, = 6) f...