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Topic:Horses

"Horses" is a broad topic that encompasses various aspects of equine biology, behavior, and management. This category includes studies on the anatomy, physiology, and genetics of horses, as well as their behavior, nutrition, and care. Research in this area may also cover the historical and cultural significance of horses, their roles in agriculture, sport, and therapy, and the challenges associated with their conservation and welfare. The page aggregates peer-reviewed research articles and scholarly studies that explore the multifaceted relationships between humans and horses, examining both scientific and socio-economic perspectives.
Detection of Cyclosporine in Horse Hair After Ocular Implant Administration.
Drug testing and analysis    August 7, 2026   doi: 10.1002/dta.70132
Lam IPY, So YM, Kwok WH, Yau W, Wong COL, Smalley SGR, Forbes BS, Chow DWY, Ho ENM.This study describes the detection and temporal distribution of cyclosporine in equine hair following ocular implant administration in a Thoroughbred gelding. Mane hair samples were collected at 172- and 217-day post-implantation and analysed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) after alkaline hydrolysis and liquid-liquid extraction. The method achieved an estimated limit of detection of 0.1 pg/mg. Cyclosporine was detected in hair at both sampling time points, with segmental analysis revealing distribution patterns consistent with systemic exposure and incorporation ...
Musculoskeletal examination of the hind limb of the horse.
Journal of the American Veterinary Medical Association    August 7, 2026   1 doi: 10.2460/javma.26.03.0234
Kamm JL, Odegaard J, Mogannam B.The intention of this tutorial video is to illustrate the methods of examining the hind limb and back of a horse in order to identify abnormalities that may be associated with lameness or disease. Unassigned: An 18-year-old quarter horse stallion was used for examination. Unassigned: The horse was examined for abnormalities using physical examination. Unassigned: The examination of the hind limb and epaxial region of the horse was successfully completed. Unassigned: The techniques described are required to identify abnormalities in the equine hind limb and can be utilized during a lameness exa...
Monitoring Estra-4,9-Diene-3,17-Dione Exposure in Equine Hair.
Drug testing and analysis    August 7, 2026   doi: 10.1002/dta.70128
Ip TKY, So YM, Kwok WH, Yuen SMS, Wong COL, Farrington AF, Bond AJ, Ho ENM.This study investigated the incorporation of estra-4,9-diene-3,17-dione (dienedione) and its metabolites into equine hair following exogenous administration and evaluates the potential application of hair analysis for long-term doping surveillance. Mane hair samples were collected from three Thoroughbred castrated horses before and after oral administration of dienedione and from untreated colts for comparison. Dienedione was detected in post-administration hair from all treated horses but was absent in corresponding pre-administration samples. Segmental distribution patterns indicated contrib...
Complications Following Nictitating Membrane Resection in Horses: 92 Eyes (2015-2024).
Veterinary ophthalmology    August 6, 2026   Volume 29, Issue 5 e70243 doi: 10.1111/vop.70243
Wu T, Stefanovski D, Scherrer NM.The goal of this retrospective study is to evaluate the complications following nictitating membrane resection in horses with the aim of providing valuable information on the risks of this surgical procedure. Methods: 83 horses (92 eyes). Methods: Medical records for 83 horses that underwent nictitating membrane resection in 92 eyes between February 2015 and June 2024 were reviewed. Horses were included if they had at least 1 month of follow-up data available. Owners were contacted via telephone or email questionnaire to obtain follow-up information. Complications were recorded only when pre...
Eight Weeks of 448 kHz Radiofrequency Therapy Increases Multifidus Muscle Cross-Sectional Area and Modulates Thoracolumbar Range of Motion in Horses with Chronic Back Pain.
Animals : an open access journal from MDPI    August 6, 2026   Volume 16, Issue 15 2437 doi: 10.3390/ani16152437
Beaussart M, Perrin R, Marunova E, Moraine F, Fourteau D, Blake R.We aimed to investigate the effects of an 8-week 448 kHz radiofrequency (RF) on multifidus cross-sectional area (CSA) and thoracolumbar differential range of motion (ROM). Eight Thoroughbred horseball horses received four RF treatments (T9-L6) at 2-week intervals. Multifidus CSA (T10-L5) and differential spinal ROM between the withers (W), T13, T18, L3 and sacrum (S) were measured before treatment, immediately after the first session, 24 h after the first session, and at 8 weeks (15 days after the last session). Linear mixed models with Tukey post hoc comparisons were used. Significant effects...
Increasing Seroprevalence In Horses Evidences Widespread Circulation Of West Nile Virus In Albania.
Veterinaria italiana    August 6, 2026   Volume 62, Issue 2 doi: 10.12834/VetIt.3956.40757.2
Lufo L, Mersini K, Llorente F, Perez Ramirez E, Jiménez-Clavero MA, Berxholi K.West Nile virus (WNV) is a zoonotic mosquito-borne flavivirus that causes West Nile disease, an emerging and re-emerging infection of major global importance. Horses are an important sentinel species for monitoring WNV circulation. Since viraemia in horses is brief and viral loads in the blood are low, serological assays are the primary tools used to monitor WNV exposure. This study aimed to estimate the seroprevalence of WNV antibodies in horses in Albania. Between 2017 and 2020, 509 serum samples were collected from apparently healthy, unvaccinated horses across different regions of the coun...
Russell’s viper envenomation in a 9-month pregnant mare saved with high-dose antivenom: a first-in-world case report.
Transactions of the Royal Society of Tropical Medicine and Hygiene    August 4, 2026   trag076 doi: 10.1093/trstmh/trag076
Raut SD, Khambatta P, Raut P, Raut S.Snakebite during pregnancy is an uncommon but potentially catastrophic event, with maternal and foetal outcomes closely related to the severity of envenomation. While snakebite in pregnant women has been sporadically reported and is known to be associated with significant obstetrical complications, envenomation in animals, particularly in pregnant horses, is exceedingly rare. Only a few isolated cases of snakebite in horses have been documented, and to the best of our knowledge, snakebite in a pregnant mare has not been previously reported in the medical or veterinary literature. Russell's vip...
Advanced 3D Horse Reconstruction: Integrating Image-to-3D Modelling and Non-Rigid Registration for On-Barn Body Measurements.
Animals : an open access journal from MDPI    August 3, 2026   Volume 16, Issue 15 2397 doi: 10.3390/ani16152397
Yuan X, Wang S, Zhu Q, Wang Y, Ruchay A, Pezzuolo A, Wang Q, Li J, Guo H.Precision livestock farming (PLF) relies on high-precision three-dimensional (3D) horse reconstruction and automatic body measurement to support refined breeding management and health surveillance. However, data collection is restricted by building environment noise and hardware layout constraints; complex equine body shapes and large individual variations induce local geometric distortions in reconstructed models, limiting field deployment. Drawing on generative 3D reconstruction, this study develops the first image-to-3D pipeline that leverages three consumer depth cameras to reconstruct hig...
Health assessment of Tokara horses on Nakanoshima island: potential chronic hepatic dysfunction induced by Leopard plant (Farfugium japonicum) ingestion.
The Journal of veterinary medical science    August 3, 2026   doi: 10.1292/jvms.26-0275
Yamashita N, Yoshihara T, Hatazoe T, Misumi K, Hobo S.We investigated the health of Tokara horses (n=34) on Nakanoshima as part of conservation efforts for this breed. Although the horses were clinically healthy, elevated white blood cell count, neutrophil count and gamma-glutamyl transferase (GGT) levels were observed, leading to suspicion of chronic hepatic dysfunction and infectious disease. A survey revealed that the caretakers had been feeding the horses Leopard plant, which contains toxic pyrrolizidine alkaloids (PAs). The abnormal GGT levels observed in these horses may suggest the possibility of chronic PA poisoning resulting from the con...
Seroprevalence, risk factors and hematological parameters associated with Equine Infectious Anemia from Casanare, Colombia.
Journal of equine veterinary science    August 3, 2026   106108 doi: 10.1016/j.jevs.2026.106108
Puentes MMM, Guzman YL, Torreglosa JCT, Isaza PAL, Guzman-Barragán BL.Equine infectious anemia (EIA) is a persistent lentiviral disease with important sanitary and economic implications in tropical regions; however, epidemiological information in Colombia remains limited. Objective: To estimate seroprevalence, identify risk factors, and evaluate hematological alterations associated with EIA in horses from Casanare, Colombia. Methods: A cross-sectional study including 1,228 horses from 204 production units across 121 rural districts. Serological diagnosis was performed using agar gel immunodiffusion. Demographic and management data were obtained through an epidem...
Comparison of xylazine-ketamine-guaifenesin and xylazine-tiletamine-zolazepam protocols for constant rate infusion anesthesia in horses undergoing field castration.
Journal of equine veterinary science    August 3, 2026   106109 doi: 10.1016/j.jevs.2026.106109
Maneephan P, Thepparat K, Suwanekkasit B, Limrodpai J, Sangbunjong A, Karkkaew P, Chantong B, Vinijkumthorn R.The common use of "triple drip" (xylazine, ketamine, and guaifenesin; XKG) is a popular protocol for constant rate infusion (CRI) in equine practice. However, due to restrictions on ketamine and guaifenesin in many countries, tiletamine-zolazepam (TZ) could serve as an alternative. To date, there have been limited studies evaluating the use of xylazine in combination with TZ (XTZ) administered via CRI to maintain surgical anesthesia in equine field practice. Objective: To compare the effectiveness and safety of XTZ infusion with the classical XKG protocol for field anesthesia. Methods: Twelve ...
Core decompression augmented with bone marrow aspirate concentrate for refractory distal sesamoid bone pathology in horses: A retrospective clinical study.
Veterinary surgery : VS    August 1, 2026   doi: 10.1111/vsu.70139
Maleas G, Mageed M, Hargitaiova K.To determine short- and long-term outcomes of core decompression combined with autologous bone marrow aspirate concentrate (BMAC) in treating moderate-to-severe refractory distal sesamoid bone (DSB) trabecular pathology in horses. Methods: Retrospective observational study. Methods: A total of 21 client-owned Warmblood horses with DSB pathology unresponsive to conservative management. Methods: Preoperative diagnosis included lameness localization by diagnostic analgesia and magnetic resonance imaging (MRI) confirmation of DSB trabecular pathology. Horses underwent arthroscopic core decompressi...
Seasonal modulation of platelet indices in mares.
Chronobiology international    July 31, 2026   1-12 doi: 10.1080/07420528.2026.2710242
Satué K, La Fauci D, Colomer-Selva R, Cravana C, Fazio E.Platelet indices provide valuable information on platelet activation, heterogeneity, and production kinetics, yet their physiological variability remains poorly characterized in horses. Although seasonal changes in platelet count have been described, the seasonal modulation of derived platelet indices in mares remains poorly understood. The aim of this study was to evaluate the seasonal variation of platelet count and derived platelet indices in clinically healthy mares from the Valencia region (Spain), and to assess their interrelationships. Forty clinically healthy Spanish Purebred mares (6-...
Retrospective endoscopic assessment of complications associated with nasogastric intubation in horses at a referral hospital.
Journal of the American Veterinary Medical Association    July 29, 2026   1-8 doi: 10.2460/javma.26.03.0206
Fielding AS, Sheahan BJ.To characterize the incidence of complications confirmed via endoscopy following nasogastric intubation in horses at a referral institution and determine associated risk factors. Unassigned: Medical records of patients having received ≥ 1 nasogastric intubation within 3 days prior to endoscopic examination between March 2021 and March 2025 were identified. Signalment, history, clinicopathologic data, nasogastric intubation procedures, reason for endoscopy, treatment, and outcome were recorded. Complications were categorized by region (nasopharyngeal, esophageal, gastric). Clinical complicati...
Approaches to Thoroughbred Horse Identification in Diverse Sample Types Using Multiplexed SNP-PCR Sequencing Panels.
Animal genetics    July 29, 2026   Volume 57, Issue 4 e70170 doi: 10.1002/age.70170
Maniego J, Harding C, Hincks P, Habershon-Butcher J, Ryder E.The ability to determine the identity of equine samples would be highly advantageous in cases of ambiguous sample source, as individual-specific controls for gene editing detection, or forensic examination of suspect biological materials in potential doping cases. To this end, a SNP-PCR sequencing-based panel comprising 93 markers in one reaction was designed and investigated for use in Thoroughbred horses. Marker amplification performance and discriminating power were calculated against a population survey of 264 Thoroughbred whole blood samples from routine doping testing. The sensitivity of...
Favorable survival of horses with guttural pouch mycosis following hospital-based treatment.
Journal of the American Veterinary Medical Association    July 29, 2026   1-6 doi: 10.2460/javma.26.04.0248
Phillips NAI, McOnie RC, Delvescovo B, Radcliffe R, Ducharme NG, Hackett ES.To report the clinical outcomes and survival time in horses undergoing treatment of guttural pouch mycosis. Unassigned: Client-owned horses with guttural pouch mycosis admitted between 2005 and 2024 for evaluation and treatment were identified by medical record search. Signalment, disease history, and surgical and treatment details were recorded. Owners were contacted for follow-up information. Unassigned: 41 horses with guttural pouch mycosis were identified, of which 28 had epistaxis or a history of epistaxis on presentation. Thirty-five horses underwent surgical treatment, and the procedure...
Differences between horses (Equus caballus) displaying oral and locomotor stereotypies in their responses to a stable mirror.
Behavioural processes    July 28, 2026   105415 doi: 10.1016/j.beproc.2026.105415
Lewis K, McBride SD, Parker MO, Faithfull R, Proops L.Recent cross-species analyses suggest that the different forms of stereotypic behaviour have heterogeneous causal mechanisms. In ungulates, there are indications that some forms of locomotor stereotypy may be linked to social factors. Using the domestic horse as a model species within this clade, we investigated whether individuals displaying the locomotor stereotypy box-walking (n=6) demonstrated greater responses to a stable mirror, intended to mimic presence of a conspecific, than horses displaying the oral stereotypy crib-biting (n=5) and non-stereotypic horses (n=5). After a 24hr baseline...
Plasma Homocysteine Concentrations in Horses with Left-Sided Valvular Heart Disease.
Animals : an open access journal from MDPI    July 26, 2026   Volume 16, Issue 15 2307 doi: 10.3390/ani16152307
Egli P, Williams Louie E, Stirn M, van Loon G, Decloedt A, Schwarzwald CC, Mitchell KJ.In human medicine, homocysteine, a sulfur-containing amino acid, is used as a biomarker reflecting endothelial dysfunction, inflammation, thrombogenesis, oxidative stress and cardiovascular disease. An automated enzyme-cycling assay for homocysteine, established for humans, has been validated and reference ranges have been created for horses. In this study the homocysteine assay was further evaluated by assessing short-term stability (whole blood and plasma samples stored at 4 °C and room temperature for 24 h), long-term stability (two years), the effect of different anticoagulants (lithium h...
Environmental and genetic effects on measured hock bone proportions in young Polish Warmblood horses undergoing performance test training for breeding horses.
Veterinary journal (London, England : 1997)    July 25, 2026   106795 doi: 10.1016/j.tvjl.2026.106795
Buczkowska R, Hecold M, Verwilghen D, Lewczuk D.The hock bone proportions in young Polish Warmblood horses (69) were studied based on x-ray image measurements done during official breeding horse performance testing. Six measurements were used to identify 4 width and length proportion indices. The radiological examinations were done before and after horse training, which consists of work with or without the rider at walk, trot and canter (40-50min) with some light class (< 100cm) jumping (<12 jumps) 6 days a week. The training effect was investigated using analysis of variance (procedure MIXED, Statistical Analysis System). The statist...
Effects of TECAR Therapy on Body Surface Temperature and Longissimus Dorsi Muscle Tone in Thoroughbred Racehorses: A Pilot Study.
Animals : an open access journal from MDPI    July 24, 2026   Volume 16, Issue 15 2300 doi: 10.3390/ani16152300
Grzegorczyk W, Soroko-Dubrovina M, Dobrowolski M, Grabska D, Dudek KD.Capacitive and resistive electric transfer (TECAR) therapy is increasingly used in equine physiotherapy; however, objective evidence regarding its immediate effects on equine back muscles remains limited. This pilot study aimed to evaluate the effects of TECAR therapy on body surface temperature and muscle tone of the longissimus dorsi muscle in Thoroughbred racehorses. A randomized, single-blinded experimental study was conducted in 20 clinically healthy Thoroughbred racehorses aged 2-3 years. Horses were randomly assigned to either a TECAR treatment group ( = 10) or a sham-treatment control ...
First experience with radiofrequency catheter ablation in horses with recurrent, persistent naturally occurring atrial fibrillation.
Equine veterinary journal    July 24, 2026   doi: 10.1002/evj.70286
Buschmann E, Van Steenkiste G, Vernemmen I, Schauvliege S, Decloedt A, Duytschaever M, van Loon G.Myocardial sleeves in the caudal vena cava and pulmonary veins are a potential trigger for initiation and perpetuation of atrial fibrillation (AF) in horses. Objective: Isolation of myocardial sleeves in the caudal vena cava or pulmonary veins might reduce the risk for AF recurrence. Methods: Case series. Methods: Records from seven horses with persistent, recurrent, naturally-occurring AF that were treated with three-dimensional electro-anatomical mapping and radiofrequency catheter ablation (RFCA) in order to reduce recurrence risk were reviewed. Mapping of the caudal vena cava was performed...
Effect of lingual gauze swab placement and light exclusion on pulse oximeter agreement with arterial haemoglobin-oxygen saturation derived from blood-gas analysis in healthy anaesthetised horses.
Veterinary anaesthesia and analgesia    July 23, 2026   Volume 53, Issue 5 101287 doi: 10.1016/j.vaa.2026.101287
Arenas Alvarez A, Martinez M, Redondo JI, Milner PI, Bardell DA.To compare peripheral arterial haemoglobin-oxygen saturation values obtained using pulse oximetry (SpO) with and without a lingual gauze swab under ambient light and light-exclusion conditions, with arterial haemoglobin-oxygen saturation derived from arterial blood-gas analysis (SaO). Methods: Prospective method-comparison study. Methods: A group of 60 healthy adult horses undergoing elective general anaesthesia. Methods: In American Society of Anesthesiologists (ASA) physical status classification I-II horses, SpO readings were obtained under six conditions during anaesthesia: no swab, no swa...
Intratumoral para-toluenesulfonamide as a tumor-ablative therapy for equine cutaneous tumors: a case series of three horses.
Veterinary and animal science    July 21, 2026   Volume 34 100777 doi: 10.1016/j.vas.2026.100777
Lo HC, Yan-Der H, Chang PL, Lin MY, Kee S.Equine neoplasia, including sarcoids, melanomas, and squamous cell carcinomas, presents therapeutic challenges due to recurrence and limitations of conventional treatments. Para-toluenesulfonamide (p-TLS) is a tumor-ablating agent that is associated with localized tumor necrosis and subsequent lesion resolution. It has been evaluated in human clinical settings as an intratumoral treatment of malignant airway obstruction caused by non-small cell lung cancer. This study aimed to describe the clinical outcomes and tolerability of intratumoral p-TLS in equine neoplasia. Three equine cases, includi...
Electrochemotherapy Following Surgical Cytoreduction for the Treatment of Recurrent Equine Sarcoids: A Retrospective Study of 23 Lesions.
Veterinary sciences    July 16, 2026   Volume 13, Issue 7 691 doi: 10.3390/vetsci13070691
Spadari A, Meistro F, Tornago R, Facchetti C, D'Angelo P, Ralletti MV, Montemagno S, Rinnovati R.Despite the wide range of therapeutic options currently available for equine sarcoids, recurrence of these lesions remains a major clinical challenge. Consequently, the identification of effective strategies for the management of recurrent sarcoids remains an important objective in equine oncology. The aim of this retrospective study was to evaluate the clinical outcome of surgical cytoreduction combined with intraoperative electrochemotherapy in horses affected by recurrent equine sarcoids. Medical records of horses treated between July 2018 and August 2025 at the Veterinary Teaching Hospital...
Mares with severe equine asthma exhibit a narrower trachea than geldings in a retrospective radiographic study of 189 horses.
American journal of veterinary research    July 16, 2026   1-10 doi: 10.2460/ajvr.26.03.0127
Arnaiz Saura P, Masseau I, Lavoie JP, Mainguy-Seers S.To evaluate potential relationships between sex, asthma severity, and tracheal diameter in adult horses through the use of thoracic radiography. Unassigned: This was a retrospective case-control study of horses that underwent thoracic radiography at the Université de Montréal from 2018 through 2024. Horses were classified as control, mild/moderate asthma, or severe asthma (SEA) according to the latest consensus statement on equine asthma. To account for radiographic magnification and body size, standardized ratios of tracheal diameter relative to the fifth thoracic vertebral body length and ...
Differential shaping of equine gut microbiota structure and function by breed and feeding regimen.
Frontiers in microbiology    July 16, 2026   Volume 17 1852554 doi: 10.3389/fmicb.2026.1852554
Lin Y, Qiri G, Du M, Dugarjaviin M, Cao J, Fang X, Yun S, Li X, Gong W, Ding W, Bou T, Su S, Chen J, Xing N, Bai D, Zhao Y.The gut microbiota plays an essential role in host energy metabolism and immune function. Horses are non-ruminant herbivores that rely heavily on hindgut microbial fermentation to meet their energy requirements. However, the relative contributions of host genetic background (breed) and environmental factors (feeding regimen and geographical location) to shaping the equine gut microbiota remain poorly understood. Unassigned: In this study, 16S rRNA gene sequencing and functional prediction analysis were performed on 139 equine fecal samples to systematically investigate the differential effects...
Preliminary Assessment on Pathogenicity of an African Horse Sickness Virus Serotype 1 Strain in Guinea Pigs and Horses.
Microorganisms    July 16, 2026   Volume 14, Issue 7 1557 doi: 10.3390/microorganisms14071557
Zhang M, Wang XF, Fu BF, Guo SF, Wang L, Zhang B, Guan FH, Song YF, Yang XY, Wang LL, Zhang QY, Yang CH.African horse sickness (AHS), a noncontagious insect-borne disease affecting equids, is caused by the African horse sickness virus (AHSV) and is associated with extremely high morbidity and mortality. AHSV has nine distinct serotypes (AHSV-1 to AHSV-9). In a previous study, we characterized the genome and in vitro growth properties of AHSV/C, a cell-adapted derivative of an AHSV-1 strain imported into China 60 years ago, and verified its safety in mice. This study preliminarily explored the pathogenicity of AHSV/C in guinea pigs and horses (its natural host). Six groups of guinea pigs were ino...
“At what line does poor horsemanship cross the line to abuse, for you?” Social media discourse around high-profile incidents in the performance horse world reveals polarised views on animal welfare.
Animal welfare (South Mimms, England)    July 15, 2026   Volume 35 e38 doi: 10.1017/awf.2026.10093
Cheung E, Mills D, Ventura BA.High profile equestrians set an example for equestrian culture as well as public perception of horse sports. The present instrumental case study considers two incidents shared across social media. One is a dressage session featuring an elite equestrian striking at a horse 24 times with a long whip during a two-minute video. The second scenario shows a high-profile rider whipping and spurring a horse for a jump refusal during an international showjumping competition. We collected (and anonymised) 416 social media responses from a performance horse welfare equestrian group, general horse welfare...
Recognition of unfamiliar predators in domestic horses through only visual predator cues.
PloS one    July 15, 2026   Volume 21, Issue 7 e0349298 doi: 10.1371/journal.pone.0349298
Hofacker R, Sebunia N, Pihlblad J, Benderlioglu Z.Acoustic, olfactory, and visual predator cues trigger various adaptive responses among a variety of prey species even when they are under human protection since birth. Previous studies have found that domesticated animals show increased vigilance, stress, flight, and aggregation behaviors in response to predation threats. These investigations, however, did not directly test visual threatening cues alone in animals that communicate more with body language than vocalizations while considering individual temperament and social status. The current study aims to address this issue. Eighteen horses ...
Prevalence and Molecular Characterisation of Cryptosporidium spp. and Giardia duodenalis in Equines in Algeria.
Veterinary medicine and science    July 15, 2026   Volume 12, Issue 4 e71083 doi: 10.1002/vms3.71083
Reghaissia N, Al-Adilee YMS, Laatamna A, Dahmane A, Berghiche A, Samari H, Ansel S, Laadjailia Y, Gentekaki E, Kváč M, Holubová N, Tsaousis AD.Limited data are available on the epidemiology of Cryptosporidium spp. and Giardia duodenalis in equines in Algeria. No prior molecular data existed on giardiasis in this host population. Objective: This cross-sectional study aimed to determine the prevalence and molecular diversity of Cryptosporidium spp. and G. duodenalis in equines across multiple regions of Algeria, and to characterise species, subtypes and assemblages present. Methods: Between December 2021 and December 2022, 197 faecal samples were collected from 104 horses, 89 donkeys, 2 ponies, 1 zebra and 1 mule from 46 private farms,...